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12-1835-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

Specific phenotypes were NOT observed.

Determination of insertion point(s)

  • 5 prime edge [+] show sequence

    1. Chromosome: 4; Position: 9271871 [Map]; Strand: c
      • AT4G16440.1 - [+] show detail - ferredoxin hydrogenases
        Distance: -202; Region: promoter
      • AT4G16442.1 - [+] show detail - Uncharacterised protein family (UPF0497)
        Distance: 0; Region: exon3 (3UTR)
    Flanking Genome Sequence: 12-1835-1 - 5-prime edge 
    TTCTTGATGA TGTTATATGT GACAAACAAT ATGTAAAACT AGACTATTGA 
    TGTTCTGTGT CAATGTGCCA TATGCTGTGA TAAAAAACGA GACTAAGGTT 
    ATTGACTTAT TGTTGAGCAA AGCATCACCC TCATTGTGTC ACCGACCTCT 
    ATTGCTTCTC TATTTGCATT TAGGCCCTTA CACAATAAGA TTATTACTAG 
    GCCCGCTATG CAATTTACAT TTTTGACTTT TTCAACTCCT TTTAGGTAAT 
    TTGAGACCCC AGTTTGCAAT TTTAGAACCA ATAGAGGAAG AAAGCACCTT 
    TCGACGCCGT TCTAGTTTTT CGATACCCTC AGCATCTCCG ACACCAATCC 
    TTAATCTTTG CTCCTTTGTT TTGGTTATAC TAGTTTCTTC GTCTCTAAAG 
    ATTANATTTT TAAGTTTTGA ATAGAAAGCG ATATGTCAGA GAAGTTTTCA 
    CCGACTTTGA GGCTCGGAGA TCTCAACGAT TTCATTGCTC CGTCTNAAGC 
    TACCANCCNN GGCCGNNN

  • 3 prime edge [+] show sequence

    1. Chromosome: 4; Position: 9271987 [Map]; Strand: c
      • AT4G16440.1 - [+] show detail - ferredoxin hydrogenases
        Distance: -318; Region: promoter
      • AT4G16442.1 - [+] show detail - Uncharacterised protein family (UPF0497)
        Distance: 0; Region: exon3 (3UTR)
    Flanking Genome Sequence: 12-1835-1 - 3-prime edge 
    ATCAAGAAGA AGAAGAAGAA GAACCAACAA GAGCCTACAC TTTTTTTACT 
    ATATGTTTTT TTAACAACAA CATTTTCAAC ACTACTAAAG ACAAAGCCGC 
    ATTCACTTCA CCACGGCGTG GTTCTGCGGT CTTTGGTGGC ACCGTACAAG 
    CGGAAGAGAC TAAAAGCAGA AATGCAGGAG ACGAAAACCA TAGCAATGGA 
    GGCTAACAAG GCGCTAGAGA CTCCTATAGC CATTTGGTTG CAGAACTTTC 
    CATACATAGT ACACACTCTC ATCCATTGCA AATCTTCCTC TCCTTCCCTA 
    GCAATCACAC CAGACTCTGC TGTTGCTGCA ATGGCTGCCA CATTCAAGTA 
    CGCCATCGCC TGCTCATTCA AAATCACAAC TACACTCTTA AATGGCCAAC 
    GAAACTTCAA TTCCAAATGC AAGCAAACAA GCAACATTCC TACCTAAGTT 
    AATTCAATTC TGATATCCAA CCAAACCCAT TTAATGCTTA GAGACTTTTC 
    AACATAGTAC CCTACTTTTC TCTAATTNGA CCTGAGATCT CAAAATAACA 
    AAGCCAAACC CCAAAAATCC CACATCCAAT TCACTAATTN TTGGCAAACA 
    AAATGTNTGG TAAAAAGAGA AACACAACTA AAACTCACCT GATCACCAGA 
    GAAAAAGGGC CCAAGCAAGA GGCTTACTAA ACAAAACCTT TCCTTCATCG 
    NACCCNCNNG CANCGNACTG ATGN

AT4G16440.1

Model type
Protein coding
Short Description
ferredoxin hydrogenases
Curator Summary
Encodes a [FeFe]-hydrogenase-like protein named Gollum (for Growth in different Oxygen LeveLs inflUences Morphogenesis). Heterologous expression of Gollum in E. coli indicates that it probably contains two [Fe-S] clusters with different magnetic properties. Sequence alignment analysis indicates that these two clusters would be topologically equivalent to the mesial and proximal [Fe-S] centers of [FeFe]-hydrogenases. Knockdown mutants (RNAi) show a dwarf phenotype at the normal atmospheric partial oxygen pressure of 21 kPa. This dwarf phenotype could be rescued by growing the plant under low oxygen pressure (5kPa), suggesting a role for this gene in oxygen sensing.
Computational Description
ferredoxin hydrogenases; CONTAINS InterPro DOMAIN/s: Iron hydrogenase, large subunit, C-terminal (InterPro:IPR004108), Iron hydrogenase (InterPro:IPR009016), Iron hydrogenase, small subunit-like (InterPro:IPR003149); Has 2574 Blast hits to 2544 proteins in 612 species: Archae - 1; Bacteria - 1826; Metazoa - 221; Fungi - 188; Plants - 72; Viruses - 0; Other Eukaryotes - 266 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT4G16442.1

Model type
Protein coding
Short Description
Uncharacterised protein family (UPF0497)
Computational Description
Uncharacterised protein family (UPF0497); FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; EXPRESSED IN: 21 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Uncharacterised protein family UPF0497, trans-membrane plant (InterPro:IPR006702), Uncharacterised protein family UPF0497, trans-membrane plant subgroup (InterPro:IPR006459); BEST Arabidopsis thaliana protein match is: Uncharacterised protein family (UPF0497) (TAIR:AT2G35760.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
Link
InterPro Scan - TAIR