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Mutant Lines
12-2658-1

12-2658-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

Anatomical entity /structure development stage	Phenotype
seedling (PO:0008037)	albino value (PATO:0000968) (Seedling::albino)

Anatomical entity
/structure development stage

Phenotype

seedling (PO:0008037)

albino value (PATO:0000968)
(Seedling::albino)

Determination of insertion point(s)

3 prime edge [+] show sequence
1. Chromosome: 1; Position: 23739744 [Map]; Strand: w
  - AT1G63970.1 - [+] show detail - isoprenoid F
    Distance: 0; Region: intron2 (CDS)
  - AT1G63970.2 - [+] show detail - isoprenoid F
    Distance: 0; Region: intron2 (CDS)
  - AT1G63980.1 - [+] show detail - D111/G-patch domain-containing protein
    Distance: -772; Region: promoter
  - AT1G63980.2 - [+] show detail - D111/G-patch domain-containing protein
    Distance: -818; Region: promoter
Flanking Genome Sequence: 12-2658-1 - 3-prime edge
```
TCAAAGAAGC TGTAAGATTT CGACGCTTAC CTTGATGTTC AATTTTGTGT 
GTATGTTCCA GTATTAGCTA CTCCTTCTTT AAGCCTGATT TTGTGTGTGG 
TACTTTATAG AATGGTTCAG AATT
```

5 prime edge [+] show sequence

Chromosome: 1; Position: 23739860 [Map]; Strand: w
- AT1G63970.1 - [+] show detail - isoprenoid F
  Distance: 0; Region: exon2 (CDS)
- AT1G63970.2 - [+] show detail - isoprenoid F
  Distance: 0; Region: exon2 (CDS)
- AT1G63980.1 - [+] show detail - D111/G-patch domain-containing protein
  Distance: -656; Region: promoter
- AT1G63980.2 - [+] show detail - D111/G-patch domain-containing protein
  Distance: -702; Region: promoter

TTCTTTGATG AATACAGAAG AAGCAGCTCC TTTCCATTTA GGATCAGAGT 
CAGGGAAAAT CTGACCTATA TCTGGAAGGC CTAGTGCTCC CAAAATTGCA 
TCCACTACAC AATGAAGTAA CACATCGCCT ACACAAATTC CAACATCAAA 
CTCAAATCAA AACCCTAAAA TCTACAAGAA GACGAATTAA CCAAATCGAA 
TTTCAAGAAA TGTTTACCAT CGGAGTGAGC TTCGCAGCCT CTATCATGAG 
GAATATCAAT CCCACCGATG ATCAGAGGAT ACCCTGGCTC TAAACGATGT 
AGATCGAAAC CATGACCGAT TCGAAACGGA AGCGTTTTGG TTGGTTTCTC 
TGAAGTCACA GATTCATTGA CGTCGACGGC GGAAGAAGCA GCTGAAACCG 
AGGCTGAAGG ACGACATGAT AACGAGAGAG ACTTCTTCGG TCTCCAAACG 
CCGATCTTCG TCGCCGGGAG AAGGAATGGC TTTTTGGTAA TTTGAGAGTA 
AAACAAAGAA GAAGAAGACA GTAGAAGCTG AGTAGAAGAA GTAGCCATTG 
CCGTTCTTCT TCGATTTTCT GGTTTACTCA ATTCATAATT CTGTGGTGGA 
AATATTCATT TCTTTATTGG GCCTTTCCTT CCTAAAGCCC ATTTAAAATA 
TGATAAACTC TCGACCGTCG ATCATGTTAC ATATCCACTG AAGTTTACTA 
AAGACCACGT GTAGAGGAGT TTTACTCATC ACTTCTTCGC TTTAGG

AT1G63970.1

Model type: Protein coding
Short Description: isoprenoid F
Curator Summary: Encodes a protein with 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity. The protein's activity was confirmed by heterologous expression of phenotypic complementation of the E. coli ispF mutant. Plants defective in this gene display an albino lethal phenotype.Homolog of E. coli IspF
Computational Description: isoprenoid F (ISPF); FUNCTIONS IN: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity; INVOLVED IN: chlorophyll biosynthetic process, carotenoid biosynthetic process, response to light stimulus, isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway; LOCATED IN: chloroplast stroma, chloroplast; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, conserved site (InterPro:IPR020555), 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, core (InterPro:IPR003526); Has 6473 Blast hits to 6472 proteins in 2098 species: Archae - 0; Bacteria - 4326; Metazoa - 0; Fungi - 4; Plants - 64; Viruses - 0; Other Eukaryotes - 2079 (source: NCBI BLink).
Link: InterPro Scan - TAIR

AT1G63970.2

Model type: Protein coding
Short Description: isoprenoid F
Curator Summary: Encodes a protein with 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity. The protein's activity was confirmed by heterologous expression of phenotypic complementation of the E. coli ispF mutant. Plants defective in this gene display an albino lethal phenotype.
Computational Description: isoprenoid F (ISPF); FUNCTIONS IN: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity; INVOLVED IN: chlorophyll biosynthetic process, carotenoid biosynthetic process, response to light stimulus, isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway; LOCATED IN: chloroplast; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, core (InterPro:IPR003526); Has 6449 Blast hits to 6448 proteins in 2092 species: Archae - 0; Bacteria - 4352; Metazoa - 0; Fungi - 4; Plants - 64; Viruses - 0; Other Eukaryotes - 2029 (source: NCBI BLink).
Link: InterPro Scan - TAIR

AT1G63980.1

Model type: Protein coding
Short Description: D111/G-patch domain-containing protein
Computational Description: D111/G-patch domain-containing protein; FUNCTIONS IN: nucleic acid binding; INVOLVED IN: biological_process unknown; LOCATED IN: intracellular; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: D111/G-patch (InterPro:IPR000467); Has 18077 Blast hits to 9113 proteins in 508 species: Archae - 9; Bacteria - 1331; Metazoa - 6165; Fungi - 2709; Plants - 1373; Viruses - 248; Other Eukaryotes - 6242 (source: NCBI BLink).
Link: InterPro Scan - TAIR

AT1G63980.2

Model type: Protein coding
Short Description: D111/G-patch domain-containing protein
Computational Description: D111/G-patch domain-containing protein; FUNCTIONS IN: nucleic acid binding; INVOLVED IN: biological_process unknown; LOCATED IN: intracellular; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: D111/G-patch (InterPro:IPR000467); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).
Link: InterPro Scan - TAIR