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12-5013-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

Specific phenotypes were NOT observed.

Determination of insertion point(s)

  • 5 prime edge [+] show sequence

    1. Chromosome: 1; Position: 26249367 [Map]; Strand: w
      • AT1G69770.1 - [+] show detail - chromomethylase 3
        Distance: 0; Region: exon17 (CDS)
    Flanking Genome Sequence: 12-5013-1 - 5-prime edge 
    GGGATAAACT AACAAAATCG GTTATACGAT AACGGTCGGT ACGGGATTTT 
    CCCATCCTAC TTTCATACCA GGTCCAACAA TAACACCAGG AAAGTCCCTA 
    AAATTCGCTC CCTGATACCA AAAGATGTTT TGAAATAATC AGAATATTCC 
    TAGACACTAA AACAGACCAG AGCCAAAAAA ATATATGATC GTCAATCGAG 
    TATAAAGAAA GTTGTACCTT TCTCTTGGGG ACCTGACAAA CCCGTTCATA 
    GTCATTTATA TTAAGATTAA GAGGGTGATG ATCATATAAG ACATGCTTTT 
    TGGACTTGGA CTTTGATTGT GAACCTGACG CTTCTGTTGA AAAAATTCAG 
    AAACAATTTG TTAATGAACA TCAGTTGCCA TAATAGTTGT CAAATATATG 
    TTTTTACACT GTTTGCCCAA AAAAATAATA AGAATCTTAC CATCCTTTCT 
    CAATCTGATG AACTTTTGAA ATGGCGTTGN GGGATCTTTG TCATATGTAA 
    TCTCGTCTCT TTTTTCACTG TTGGCAACCT AATACAAAAA CATAGGAAGT 
    AAGAAAATAT TCAAGACAAA CTGTACCTAA TTTTAGTCAA AATTTGCTAA 
    AAACATCAAT ACTTACTGNN GGAAGATCAG AAATCACATN CTTNACAAAA 
    GCTACNANCC NGGGCCGTNG ACCCGCGNNC CCNTNNA

  • 3 prime edge [+] show sequence

    1. Chromosome: 1; Position: 26249358 [Map]; Strand: w
      • AT1G69770.1 - [+] show detail - chromomethylase 3
        Distance: 0; Region: exon17 (CDS)
    Flanking Genome Sequence: 12-5013-1 - 3-prime edge 
    ATGAAAACGG TAGAGGTATT TTACCGACCG TTACCGACCG TTTTCATCCA 
    ATGTAGTCAA GTTGGAAGAG GGAAAGGAAA GGGTCAAACT TGAATCTGGA 
    AAAACATTGG TAAGAATGAT CATCTCTTTT TTTTGGTCTC ATATCAAGTT 
    ACTTTTCTCC TGATTGGCGT GGCTATGTTA TTCAAACAGG TTCCCGATTA 
    TGCCTTAACA TATGTCGATG GGAAATCATG CAAGTGAGTT TTAATTCTCT 
    TTTCTTCGTC TCTGAAAATT CTGTATTTGA CTATTGTTTT CTGTTATGGA 
    AATTGACTTA AGCCCTTCGC TGTTTCCANA CCTTTTGGTC GTCTTTGGTG 
    GGACGAAATT GTCCCCACTG TTGTCACACG GGCAGANCCC CACAACCANG 
    TGAGACCTCT CATGACAATC AAGTATTTGT GTCTGGATTT TGGCTAGNTG 
    TTATNCTANT GNTCTAGGTT TGCATANATT TATTAGTATG CATGTTACAC 
    TGGAACCANC TTTANAGANT NTAAAAGNTN NATGTANCGT GNCGATNGAA 
    TTGCANGNTG ATNCNTNNCA TCCCNGANCT NCNTCNGNTT NNANNCCTTT 
    CNCAAAATNT NCGNANNCTT NCAGGGGNTT TCCCTNNNTN NCTTANTNAN 
    NTCTTTGNCC CGNCCCNNCC NNAAGNCAAN TATCGGTNNA AAANNGNNGN 
    NNTTANGNGN CGNGCCNNGN NGTNNNCATG TACCTNNGAA GTNANNNGNN 
    NNNTCNGNGT CNTTNTATNN TGAGNGNACN NCTTGNNTCT NGNGN

AT1G69770.1

Model type
Protein coding
Short Description
chromomethylase 3
Curator Summary
Encodes a chromomethylase involved in methylating cytosine residues at non-CG sites. Involved in preferentially methylating transposon-related sequences, reducing their mobility. CMT3 interacts with an Arabidopsis homologue of HP1 (heterochromatin protein 1), which in turn interacts with methylated histones. Involved in gene silencing.
Computational Description
chromomethylase 3 (CMT3); FUNCTIONS IN: DNA (cytosine-5-)-methyltransferase activity; INVOLVED IN: in 6 processes; LOCATED IN: chromatin, nucleus; EXPRESSED IN: 21 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: DNA methylase, C-5 cytosine-specific (InterPro:IPR001525), Chromo domain-like (InterPro:IPR016197), Bromo adjacent homology (BAH) domain (InterPro:IPR001025), DNA methylase, C-5 cytosine-specific, active site (InterPro:IPR018117), Chromo domain (InterPro:IPR000953); BEST Arabidopsis thaliana protein match is: chromomethylase 2 (TAIR:AT4G19020.1); Has 4337 Blast hits to 3739 proteins in 974 species: Archae - 208; Bacteria - 2545; Metazoa - 230; Fungi - 161; Plants - 416; Viruses - 30; Other Eukaryotes - 747 (source: NCBI BLink).
Link
InterPro Scan - TAIR