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12-5058-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

Specific phenotypes were NOT observed.

Determination of insertion point(s)

  • 5 prime edge [+] show sequence

    1. Chromosome: 5; Position: 7853910 [Map]; Strand: w
      • AT5G23320.1 - [+] show detail - homolog of yeast STE14 A
        Distance: 0; Region: exon1 (CDS)
      • AT5G23330.1 - [+] show detail - Nucleotidylyl transferase superfamily protein
        Distance: -219; Region: promoter
    Flanking Genome Sequence: 12-5058-1 - 5-prime edge 
    GTCGTGTGGC GGTTTTTTGC TCAGAGAATA CCGTACGAGG AGTATTTTCT 
    GAATCAGTTT TTTGGGGTAC AGTATCTAGA GTATGCAGAG AGTGTTGCCT 
    CTGGTGTTCC ATTTGTGAAC TGACACTTTG AATTATTTTT GAACATATGA 
    GTTTGATGTA CTTGACTTGA GGTCTTAAAG TCACAAAAAC ATACAAGACC 
    CTCTGGCTGA AAGAGAAGGT CTTGACGTGG ATATGAAATT TACCACAGCG 
    TTAATTGTTT ATGTGACACC TGTCACCGGT GATGCATAGC TCCGGCGAAG 
    AGTTTCGGAG AACAAGTTTG GTCTTTTTAG GGGTTTGAGC AGTGGAGTCG 
    GTAACCATGT TATGCGGAGG CTCTCGCGCC TCCGTGCATT TGTGGGACCA 
    TCGTCATCCT CCTAGGCTCG GTGCTAAGGT TTTGCGGAAA TCTTCGTTCA 
    TGCTCAGACC TTGTTCGGCT ATATCGCAGC AGCGGATAAA GTCGTCGTTT 
    AGGTCTCACT GTAAAACTCC AAGAAAAATC CCAGCTCCTC TCGATTGTTT 
    CAGGTTTTTG AATCTCGCTG TTGTTTTGAT TGGTTTTTGA TCCGAATCTG 
    ATTGTNTTAT GTGAAATTTC CTGATGAGCT TTAANATAGA NATTTNCTGN 
    NANANGNTCT TCACTATGNT CTACNTCAAT TTTCAAAATN GGANTCTTTA 
    CCA

  • 3 prime edge [+] show sequence

    1. Chromosome: 5; Position: 7853794 [Map]; Strand: w
      • AT5G23320.1 - [+] show detail - homolog of yeast STE14 A
        Distance: 0; Region: exon1 (CDS)
      • AT5G23330.1 - [+] show detail - Nucleotidylyl transferase superfamily protein
        Distance: -335; Region: promoter
    Flanking Genome Sequence: 12-5058-1 - 3-prime edge 
    CACACGACAA CCGCGAACGC AACTGCTGAA ACGGGGTTAC AGAGCATAAC 
    TTGTGTCCCG ACCGACCAGA TGAGAAAACC GCAGTAACTT GGATGCCTCA 
    TTAGTCTATA CACACCATGA GTCACAAGCC CGTGATGCTC TTCGTAGTTG 
    ATCTTTATGA GGTGAGTGAA CGATCTTCCC GCTGTTATTA TCGCTGCCTT 
    CCTGATGATT TCCCCAACGA TGATCATTAT GAGTCCAAAG TTGCTGACCC 
    ACCAGTGTTG TTTCAGCCCC GGGAAGAGGA TAATCTCCGT TAGGTATTCG 
    AGAAGCGACA GAAGCATTGC TAAAGCGTAA TGCTTGGTGA TTAAAAGCGA 
    ACTAAGAGTT ACGTTTGATG CTCCGTGAAT GGTGATGGCT AGAATGTATT 
    CGGATATGTG GAAGAAGATT AGTGATAGTA GCATTTGAGA TAACTGTCTG 
    ATGCTGGTGT CACTGAAGAT CTCTGTCATT TTTATCAATC AAGAAAGATT 
    ATAAGAGGTT TTCTTTCCTG TTTGATCAAA GCT

AT5G23320.1

Model type
Protein coding
Short Description
homolog of yeast STE14 A
Curator Summary
Encodes a prenylcysteine alpha-carboxyl methyltransferase involved in methylation of isoprenylated proteins. This protein appears to have lower catalytic activity and a lower transcript expression level than the other ICMT present in Arabidopsis (At5g08335). Analysis of ICMT RNAi lines suggests that this protein may be involved in flower and stem development.
Computational Description
homolog of yeast STE14 A (STE14A); CONTAINS InterPro DOMAIN/s: Isoprenylcysteine carboxyl methyltransferase (InterPro:IPR007269); BEST Arabidopsis thaliana protein match is: Isoprenylcysteine carboxyl methyltransferase (ICMT) family (TAIR:AT5G08335.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT5G23330.1

Model type
Protein coding
Short Description
Nucleotidylyl transferase superfamily protein
Computational Description
Nucleotidylyl transferase superfamily protein; FUNCTIONS IN: FMN adenylyltransferase activity; INVOLVED IN: riboflavin biosynthetic process; EXPRESSED IN: 13 plant structures; EXPRESSED DURING: 4 anthesis, F mature embryo stage, petal differentiation and expansion stage, E expanded cotyledon stage, D bilateral stage; CONTAINS InterPro DOMAIN/s: Rossmann-like alpha/beta/alpha sandwich fold (InterPro:IPR014729), FAD synthetase (InterPro:IPR015864); BEST Arabidopsis thaliana protein match is: Nucleotidylyl transferase superfamily protein (TAIR:AT5G08340.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
Link
InterPro Scan - TAIR