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Mutant Lines
12-5255-1

12-5255-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

This line has NOT been observed.

Determination of insertion point(s)

5 prime edge [+] show sequence
1. Chromosome: 4; Position: 220882 [Map]; Strand: c
  - AT4G00480.1 - [+] show detail - basic helix-loop-helix (bHLH) DNA-binding superfamily protein
    Distance: -1139; Region: intergenic
  - AT4G00490.1 - [+] show detail - beta-amylase 2
    Distance: -1438; Region: intergenic
Flanking Genome Sequence: 12-5255-1 - 5-prime edge
```
CCACTTGCTA TATTTACTTA CTTCGGACAA AATAGTGCTT TGATGGTAAA 
CAAATAACGA TCGGTATATG CGATGTCTCG CGATTGATTA TCAAACTTGA 
ACAGTAGTGT GTATTTGTTT AATATATAAT CTATTAGAGT ATCATGGAAT 
TATTCGGGTG AAATGCGCAT TTATATCTAT CATAGAAACT AGAATCTTC
```

3 prime edge [+] show sequence

Chromosome: 4; Position: 220998 [Map]; Strand: c
- AT4G00480.1 - [+] show detail - basic helix-loop-helix (bHLH) DNA-binding superfamily protein
  Distance: -1255; Region: intergenic
- AT4G00490.1 - [+] show detail - beta-amylase 2
  Distance: -1322; Region: intergenic

GCAAGTGGCA GTAAAAAAAT AGTAATAAAA TTGAAGGCAA GATCAGGAGA 
GAGCTGGCTG AAATGAAGGC AAGAAACTCG TGAAAATAAT AATTGATCCA 
TCTCTATCCA CGAAGCCCTA CATAATTTAG TAACGCACCA CTTGTGGTTG 
CATGCACATT TATTTCCATA ACGACAAAAC TTTGTGTTTA ATTTTATAAA 
TTATTTCTGT TGGCACAACA CACTCATTAT AGCCATATGT TTGTGTTTAT 
ATTTTCTATT TTCATAGAAA CAATTAATTT AATTTTAGAT GCCCTAGACA 
TTTTTTAAAT GTATATAATA ATAAAATTAT AGTATAAAAG TCTATATATT 
ACCCTAAATT TTACAAAACA TATACAATCC TAACCAATGG GATC

AT4G00480.1

Model type: Protein coding
Short Description: basic helix-loop-helix (bHLH) DNA-binding superfamily protein
Curator Summary: MYC-related protein with a basic helix-loop-helix motif at the C-terminus and a region similar to the maize B/R family at the N-terminus
Computational Description: ATMYC1; FUNCTIONS IN: DNA binding, sequence-specific DNA binding transcription factor activity; INVOLVED IN: regulation of transcription; LOCATED IN: nucleus; EXPRESSED IN: 21 plant structures; EXPRESSED DURING: 12 growth stages; CONTAINS InterPro DOMAIN/s: Helix-loop-helix DNA-binding domain (InterPro:IPR001092), Helix-loop-helix DNA-binding (InterPro:IPR011598); BEST Arabidopsis thaliana protein match is: basic helix-loop-helix (bHLH) DNA-binding superfamily protein (TAIR:AT1G63650.3); Has 1473 Blast hits to 1082 proteins in 120 species: Archae - 0; Bacteria - 0; Metazoa - 3; Fungi - 0; Plants - 1458; Viruses - 0; Other Eukaryotes - 12 (source: NCBI BLink).
Link: InterPro Scan - TAIR

AT4G00490.1

Model type: Protein coding
Short Description: beta-amylase 2
Curator Summary: Encodes a chloroplast beta-amylase. The enzyme activity is very weak compared to BAM1 and BAM3. Mutant of BAM2 has no visible phenotype.
Computational Description: beta-amylase 2 (BAM2); FUNCTIONS IN: beta-amylase activity; INVOLVED IN: carbohydrate metabolic process, polysaccharide catabolic process; LOCATED IN: chloroplast; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Glycoside hydrolase, family 14, conserved site (InterPro:IPR018238), Glycoside hydrolase, family 14 (InterPro:IPR001554), Glycoside hydrolase, catalytic core (InterPro:IPR017853), Glycoside hydrolase, family 14B, plant (InterPro:IPR001371), Glycoside hydrolase, subgroup, catalytic core (InterPro:IPR013781); BEST Arabidopsis thaliana protein match is: beta-amylase 7 (TAIR:AT2G45880.1); Has 836 Blast hits to 835 proteins in 165 species: Archae - 0; Bacteria - 84; Metazoa - 0; Fungi - 0; Plants - 686; Viruses - 0; Other Eukaryotes - 66 (source: NCBI BLink).
Link: InterPro Scan - TAIR