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13-0289-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

This line has NOT been observed.

Determination of insertion point(s)

  • 5 prime edge [+] show sequence

    1. Chromosome: 4; Position: 8051583 [Map]; Strand: c
      • AT4G13930.1 - [+] show detail - serine hydroxymethyltransferase 4
        Distance: -1477; Region: intergenic
      • AT4G13940.3 - [+] show detail - S-adenosyl-L-homocysteine hydrolase
        Distance: -3278; Region: intergenic
    Flanking Genome Sequence: 13-0289-1 - 5-prime edge
    AATAGATCAT GCCATTCTNT AGCAAATATT ANATACTTAA TATTCTTTGA 
    TCAACCGAGG GCACGATGCA ATAATAATAA ATAAATTTGT TAACCATAAC
    AACTCCTCAA TCAGTGTAGT GCGTTAGTTA ATTAGTATGT CCACGAAAAA
    AGGTTTCAAA GGGAGGCTAC CAACTATTTC TTGTTTATAA AAATGTGTGT
    TTAATGGCTC TTTTCATATG CTAAATTATA AAATTTGTGA CAGAGAATAT
    CTAAGACTTT AGTGGAATAA CAAACTTATT TTTTCTCATT AGAAACTGAG
    AGTATCGAAA AATAACAATT TGTACACATT TCATTTGGCA TTTTCAACAT
    AAGCAAAATG GGCGAAAGAG TTGGATATGT TCTCTAACTA TAATGTGATG
    TTAATTTGGA TATGGTATCG ATTAAGTGAT CTAGTCAGGA TGCTAAGCAA
    AGACCACCAA TTTCTTATAA TGTTGGGATA ATGAATAGTG AGGCAAAGTG
    ATGCTGTAGT TACACATATG ATGAGTTGTC ACTGAAATTA TAACATAAAC
    ATAAACAAGG ATATAGTCTT ATAGANGGTA CGGGTACACA TATGATGAGT
    TGTCATTGAA AATTATTACN TAAACATAAC AAGGATNTAG TCATATANAN
    GGTACNGGGA CTTCGNGATT CTTAGNGATG ATTGATGAAT ATGAACTAAN
    ATCATNCNCA GGTACTTTAA AANAAGATN
  • 3 prime edge [+] show sequence

    1. Chromosome: 4; Position: 8051650 [Map]; Strand: c
      • AT4G13930.1 - [+] show detail - serine hydroxymethyltransferase 4
        Distance: -1544; Region: intergenic
      • AT4G13940.3 - [+] show detail - S-adenosyl-L-homocysteine hydrolase
        Distance: -3211; Region: intergenic
    Flanking Genome Sequence: 13-0289-1 - 3-prime edge
    ATGAAAACGG TAGAGGTATT TTACCGACCG TTACCGACCG TTTTCATCCA 
    ATCATGGGGT TTGGAGAGGA GAGTAGGTGG TTTTGTATGC ATGTGAGTCG
    GTGAGGGTCA AAATCGTCTT TTCAATTTAA GATTTGATTA TGTGGTCATT
    AAATAGTAAC GTAGTGCCTA GTGGTATTGG CAGATCTGAG GTCAACATGT
    GATGTGACGA AGATCTTCAT TTTCTTCGTA AACACAAAGA ATACTGAAGT
    TAGAAAAATG TCTTTTTCAG GTAATATTTA CTCAGTAAAG AAAAAAGGCT
    GGTAAAAGTA AACTTTTAAG TTTGAACGTT AAGTCAATTT AATAGTGTTT
    ATTTATTTAT TCGACATGTA GAAAATTAAA CGATCTACGT AAAATATGAA
    TACAAAAATA TTAAATATTA GAATAAGTGA GACTGTGAGA GTATGTGTAA
    ATCACGTTTT GGTGAATTTT TTCCATGTGG TAGAATAATA GAGGAGGACT
    TTTGTTGTAA AGTAAGCT

AT4G13930.1

Model type
Protein coding
Short Description
serine hydroxymethyltransferase 4
Curator Summary
Encodes a serine hydroxymethyltransferase maximally expressed in root
Computational Description
serine hydroxymethyltransferase 4 (SHM4); FUNCTIONS IN: pyridoxal phosphate binding, glycine hydroxymethyltransferase activity, catalytic activity; INVOLVED IN: response to cadmium ion, glycine metabolic process, L-serine metabolic process; LOCATED IN: cytosol, plasma membrane, membrane; EXPRESSED IN: 9 plant structures; EXPRESSED DURING: seedling growth, seed development stages; CONTAINS InterPro DOMAIN/s: Pyridoxal phosphate-dependent transferase, major domain (InterPro:IPR015424), Serine hydroxymethyltransferase, pyridoxal phosphate binding site (InterPro:IPR019798), Pyridoxal phosphate-dependent transferase, major region, subdomain 1 (InterPro:IPR015421), Serine hydroxymethyltransferase (InterPro:IPR001085); BEST Arabidopsis thaliana protein match is: Pyridoxal phosphate (PLP)-dependent transferases superfamily protein (TAIR:AT4G13890.1); Has 11689 Blast hits to 11661 proteins in 2862 species: Archae - 259; Bacteria - 6437; Metazoa - 341; Fungi - 289; Plants - 375; Viruses - 6; Other Eukaryotes - 3982 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT4G13940.3

Model type
Protein coding
Short Description
S-adenosyl-L-homocysteine hydrolase
Curator Summary
Encodes a S-adenosyl-L-homocysteine hydrolase required for DNA methylation-dependent gene silencing.
Computational Description
HOMOLOGY-DEPENDENT GENE SILENCING 1 (HOG1); FUNCTIONS IN: adenosylhomocysteinase activity, copper ion binding; INVOLVED IN: methylation-dependent chromatin silencing, one-carbon metabolic process, posttranscriptional gene silencing, embryo development ending in seed dormancy; LOCATED IN: plasma membrane, membrane; EXPRESSED IN: 35 plant structures; EXPRESSED DURING: 16 growth stages; CONTAINS InterPro DOMAIN/s: S-adenosyl-L-homocysteine hydrolase (InterPro:IPR000043), S-adenosyl-L-homocysteine hydrolase, conserved site (InterPro:IPR020082), S-adenosyl-L-homocysteine hydrolase, NAD binding (InterPro:IPR015878); BEST Arabidopsis thaliana protein match is: S-adenosyl-l-homocysteine (SAH) hydrolase 2 (TAIR:AT3G23810.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).
Link
InterPro Scan - TAIR