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Mutant Lines
13-0289-1

13-0289-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

This line has NOT been observed.

Determination of insertion point(s)

5 prime edge [+] show sequence

Chromosome: 4; Position: 8051583 [Map]; Strand: c
- AT4G13930.1 - [+] show detail - serine hydroxymethyltransferase 4
  Distance: -1477; Region: intergenic
- AT4G13940.3 - [+] show detail - S-adenosyl-L-homocysteine hydrolase
  Distance: -3278; Region: intergenic

AATAGATCAT GCCATTCTNT AGCAAATATT ANATACTTAA TATTCTTTGA 
TCAACCGAGG GCACGATGCA ATAATAATAA ATAAATTTGT TAACCATAAC 
AACTCCTCAA TCAGTGTAGT GCGTTAGTTA ATTAGTATGT CCACGAAAAA 
AGGTTTCAAA GGGAGGCTAC CAACTATTTC TTGTTTATAA AAATGTGTGT 
TTAATGGCTC TTTTCATATG CTAAATTATA AAATTTGTGA CAGAGAATAT 
CTAAGACTTT AGTGGAATAA CAAACTTATT TTTTCTCATT AGAAACTGAG 
AGTATCGAAA AATAACAATT TGTACACATT TCATTTGGCA TTTTCAACAT 
AAGCAAAATG GGCGAAAGAG TTGGATATGT TCTCTAACTA TAATGTGATG 
TTAATTTGGA TATGGTATCG ATTAAGTGAT CTAGTCAGGA TGCTAAGCAA 
AGACCACCAA TTTCTTATAA TGTTGGGATA ATGAATAGTG AGGCAAAGTG 
ATGCTGTAGT TACACATATG ATGAGTTGTC ACTGAAATTA TAACATAAAC 
ATAAACAAGG ATATAGTCTT ATAGANGGTA CGGGTACACA TATGATGAGT 
TGTCATTGAA AATTATTACN TAAACATAAC AAGGATNTAG TCATATANAN 
GGTACNGGGA CTTCGNGATT CTTAGNGATG ATTGATGAAT ATGAACTAAN 
ATCATNCNCA GGTACTTTAA AANAAGATN

3 prime edge [+] show sequence

Chromosome: 4; Position: 8051650 [Map]; Strand: c
- AT4G13930.1 - [+] show detail - serine hydroxymethyltransferase 4
  Distance: -1544; Region: intergenic
- AT4G13940.3 - [+] show detail - S-adenosyl-L-homocysteine hydrolase
  Distance: -3211; Region: intergenic

ATGAAAACGG TAGAGGTATT TTACCGACCG TTACCGACCG TTTTCATCCA 
ATCATGGGGT TTGGAGAGGA GAGTAGGTGG TTTTGTATGC ATGTGAGTCG 
GTGAGGGTCA AAATCGTCTT TTCAATTTAA GATTTGATTA TGTGGTCATT 
AAATAGTAAC GTAGTGCCTA GTGGTATTGG CAGATCTGAG GTCAACATGT 
GATGTGACGA AGATCTTCAT TTTCTTCGTA AACACAAAGA ATACTGAAGT 
TAGAAAAATG TCTTTTTCAG GTAATATTTA CTCAGTAAAG AAAAAAGGCT 
GGTAAAAGTA AACTTTTAAG TTTGAACGTT AAGTCAATTT AATAGTGTTT 
ATTTATTTAT TCGACATGTA GAAAATTAAA CGATCTACGT AAAATATGAA 
TACAAAAATA TTAAATATTA GAATAAGTGA GACTGTGAGA GTATGTGTAA 
ATCACGTTTT GGTGAATTTT TTCCATGTGG TAGAATAATA GAGGAGGACT 
TTTGTTGTAA AGTAAGCT

AT4G13930.1

Model type: Protein coding
Short Description: serine hydroxymethyltransferase 4
Curator Summary: Encodes a serine hydroxymethyltransferase maximally expressed in root
Computational Description: serine hydroxymethyltransferase 4 (SHM4); FUNCTIONS IN: pyridoxal phosphate binding, glycine hydroxymethyltransferase activity, catalytic activity; INVOLVED IN: response to cadmium ion, glycine metabolic process, L-serine metabolic process; LOCATED IN: cytosol, plasma membrane, membrane; EXPRESSED IN: 9 plant structures; EXPRESSED DURING: seedling growth, seed development stages; CONTAINS InterPro DOMAIN/s: Pyridoxal phosphate-dependent transferase, major domain (InterPro:IPR015424), Serine hydroxymethyltransferase, pyridoxal phosphate binding site (InterPro:IPR019798), Pyridoxal phosphate-dependent transferase, major region, subdomain 1 (InterPro:IPR015421), Serine hydroxymethyltransferase (InterPro:IPR001085); BEST Arabidopsis thaliana protein match is: Pyridoxal phosphate (PLP)-dependent transferases superfamily protein (TAIR:AT4G13890.1); Has 11689 Blast hits to 11661 proteins in 2862 species: Archae - 259; Bacteria - 6437; Metazoa - 341; Fungi - 289; Plants - 375; Viruses - 6; Other Eukaryotes - 3982 (source: NCBI BLink).
Link: InterPro Scan - TAIR

AT4G13940.3

Model type: Protein coding
Short Description: S-adenosyl-L-homocysteine hydrolase
Curator Summary: Encodes a S-adenosyl-L-homocysteine hydrolase required for DNA methylation-dependent gene silencing.
Computational Description: HOMOLOGY-DEPENDENT GENE SILENCING 1 (HOG1); FUNCTIONS IN: adenosylhomocysteinase activity, copper ion binding; INVOLVED IN: methylation-dependent chromatin silencing, one-carbon metabolic process, posttranscriptional gene silencing, embryo development ending in seed dormancy; LOCATED IN: plasma membrane, membrane; EXPRESSED IN: 35 plant structures; EXPRESSED DURING: 16 growth stages; CONTAINS InterPro DOMAIN/s: S-adenosyl-L-homocysteine hydrolase (InterPro:IPR000043), S-adenosyl-L-homocysteine hydrolase, conserved site (InterPro:IPR020082), S-adenosyl-L-homocysteine hydrolase, NAD binding (InterPro:IPR015878); BEST Arabidopsis thaliana protein match is: S-adenosyl-l-homocysteine (SAH) hydrolase 2 (TAIR:AT3G23810.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).
Link: InterPro Scan - TAIR