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Mutant Lines
13-0844-1

13-0844-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

This line has NOT been observed.

Determination of insertion point(s)

5 prime edge [+] show sequence

Chromosome: 1; Position: 3041895 [Map]; Strand: w
- AT1G09420.1 - [+] show detail - glucose-6-phosphate dehydrogenase 4
  Distance: -76; Region: promoter
- AT1G09420.2 - [+] show detail - glucose-6-phosphate dehydrogenase 4
  Distance: -22; Region: promoter
- AT1G09430.1 - [+] show detail - ATP-citrate lyase A-3
  Distance: -101; Region: promoter

TCCATTAATG GTTCCAACTT TCAGATAATC GTCGGGTTCT TCTAAACCGA 
ACCGTACCGG TTGGTTCTCG AGGAAAGAGA ATTGAATTTG TGGTTTAGGC 
GGGGGTGGGT AGGTTGACCT TTCGATATCT CAACGTCAAC NGTCTCTTCT 
GGTCCCGCCG AGTCGGCGTC ATCGTTGAGT TGGTGAGATC ATCTTCTTCT 
CAACTTCATC GATCTTATCG TCTCGAAAAT TGTCACCTTT TCTTCTCAGC 
TACTTCAGGC GGAGAAACAC TTTCCGTGGT TTAGTGTTGA GGACGATCAC 
TGTTTGTAAT TCGATGGCGA GGAAGAAGAT CAGAGAATAT GACTCCAAGA 
GACTTCTCAA GGAGCATTTG AAGCGTCTTG CTAATATTGA CCTGCAGATT 
CGCTCTGCTC AGGTCATTCT CACGAATT

3 prime edge [+] show sequence
1. Chromosome: 1; Position: 3041779 [Map]; Strand: w
  - AT1G09420.1 - [+] show detail - glucose-6-phosphate dehydrogenase 4
    Distance: 0; Region: exon1 (5UTR)
  - AT1G09420.2 - [+] show detail - glucose-6-phosphate dehydrogenase 4
    Distance: 0; Region: exon1 (5UTR)
  - AT1G09430.1 - [+] show detail - ATP-citrate lyase A-3
    Distance: -217; Region: promoter
Flanking Genome Sequence: 13-0844-1 - 3-prime edge
```
TTAATGGAGG CGTCAATCAG AAAACTTAAA CTCGCACTCG TCTTCCACTT 
TGATCCACTT AAAGCAGCTT TAAAGCT
```

AT1G09420.1

Model type: Protein coding
Short Description: glucose-6-phosphate dehydrogenase 4
Curator Summary: Encodes a protein similar to glucose-6-phosphate dehydrogenase but, based on amino acid differences in the active site and lack of activity, does not encode a functional G6PDH. The amino acid sequence for the consensus sequence of the G6PDH active site (DHYLGKE) differs in three places in this protein. gc exon splice site at 20574 is based on protein alignment, and is not confirmed experimentally.
Computational Description: glucose-6-phosphate dehydrogenase 4 (G6PD4); FUNCTIONS IN: glucose-6-phosphate dehydrogenase activity; INVOLVED IN: oxidation reduction, glucose metabolic process, metabolic process; LOCATED IN: chloroplast; EXPRESSED IN: 14 plant structures; EXPRESSED DURING: 6 growth stages; CONTAINS InterPro DOMAIN/s: Glucose-6-phosphate dehydrogenase, C-terminal (InterPro:IPR022675), NAD(P)-binding domain (InterPro:IPR016040), Glucose-6-phosphate dehydrogenase (InterPro:IPR001282), Glucose-6-phosphate dehydrogenase, NAD-binding (InterPro:IPR022674); BEST Arabidopsis thaliana protein match is: glucose-6-phosphate dehydrogenase 2 (TAIR:AT5G13110.1); Has 8248 Blast hits to 8228 proteins in 2347 species: Archae - 0; Bacteria - 5681; Metazoa - 875; Fungi - 180; Plants - 375; Viruses - 4; Other Eukaryotes - 1133 (source: NCBI BLink).
Link: InterPro Scan - TAIR

AT1G09420.2

Model type: Protein coding
Short Description: glucose-6-phosphate dehydrogenase 4
Curator Summary: Encodes a protein similar to glucose-6-phosphate dehydrogenase but, based on amino acid differences in the active site and lack of activity, does not encode a functional G6PDH. The amino acid sequence for the consensus sequence of the G6PDH active site (DHYLGKE) differs in three places in this protein. gc exon splice site at 20574 is based on protein alignment, and is not confirmed experimentally.
Computational Description: glucose-6-phosphate dehydrogenase 4 (G6PD4); FUNCTIONS IN: glucose-6-phosphate dehydrogenase activity; INVOLVED IN: oxidation reduction, glucose metabolic process, metabolic process; LOCATED IN: chloroplast; EXPRESSED IN: 14 plant structures; EXPRESSED DURING: 6 growth stages; CONTAINS InterPro DOMAIN/s: Glucose-6-phosphate dehydrogenase, C-terminal (InterPro:IPR022675), NAD(P)-binding domain (InterPro:IPR016040), Glucose-6-phosphate dehydrogenase (InterPro:IPR001282), Glucose-6-phosphate dehydrogenase, NAD-binding (InterPro:IPR022674); BEST Arabidopsis thaliana protein match is: glucose-6-phosphate dehydrogenase 2 (TAIR:AT5G13110.1).
Link: InterPro Scan - TAIR

AT1G09430.1

Model type: Protein coding
Short Description: ATP-citrate lyase A-3
Curator Summary: Encodes subunit A of the heteromeric enzyme ATP citrate lyase (ACL). In animals, ACL is encoded by a single gene; ACL in Arabidopsis is composed of two polypeptides, ACLA (encoded by 3 genes) and ACLB (encoded by 2 genes). The holoenzyme has an A(4)B(4)stoichiometry. Expression of both ACLA and ACLB but not of either of the subunits alone results in ACL activity.
Computational Description: ATP-citrate lyase A-3 (ACLA-3); CONTAINS InterPro DOMAIN/s: ATP-grasp fold, subdomain 2 (InterPro:IPR013816), ATP-grasp fold, succinyl-CoA synthetase-type (InterPro:IPR013650), Succinyl-CoA synthetase-like (InterPro:IPR016102); BEST Arabidopsis thaliana protein match is: ATP-citrate lyase A-1 (TAIR:AT1G10670.4); Has 5303 Blast hits to 5302 proteins in 1635 species: Archae - 133; Bacteria - 3235; Metazoa - 230; Fungi - 139; Plants - 96; Viruses - 0; Other Eukaryotes - 1470 (source: NCBI BLink).
Link: InterPro Scan - TAIR