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13-1164-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

Anatomical entity
/structure development stage		 Phenotype
vascular leaf (PO:0009025)
  • desaturated green (PATO:0001272)
    (Leaves::pale green)

Determination of insertion point(s)

  • 3 prime edge [+] show sequence

    1. Chromosome: 4; Position: 10202029 [Map]; Strand: c
      • AT4G18480.1 - [+] show detail - P-loop containing nucleoside triphosphate hydrolases superfamily protein
        Distance: 0; Region: exon3 (CDS)
    Flanking Genome Sequence: 13-1164-1 - 3-prime edge 
    GCCTTAAGCA GTTAGGGATA ACGGTTGCAA CATCATCTGG AGTTACTCGA 
    TCTTTTCCTT TGAGAGCTGC AAGTGCTTTT GCTGCTCTGT TAGTCACTAT 
    GTCTCCTCTC AACCCATCAA CATTGAGCTC TGAACAAACT CTAGAGATCT 
    TCACCTTCAA TTCCCTATCA ATCTGAACCG AGGAAAGGTT TGCCCTAGCA 
    GTTGAAATCT GGTCTTGAAG CT

  • 5 prime edge [+] show sequence

    1. Chromosome: 4; Position: 10201976 [Map]; Strand: c
      • AT4G18480.1 - [+] show detail - P-loop containing nucleoside triphosphate hydrolases superfamily protein
        Distance: 0; Region: exon3 (CDS)
    Flanking Genome Sequence: 13-1164-1 - 5-prime edge 
    GGGATAAACT AACAAAATCG GTTATACGAT AACGGTCGGT ACGGGATTTT 
    CCCATCCTAC TTTCATCAGG CACCGTCTGA GGAAAGATCC ACTGGAATCT 
    ATTGATTCAG GAGTTCTAGT TTCCGAGAAG TTCGCCGAGA TTTTCAGCTG 
    AATAGGAGAT TCGATTCTGT GATCATTTTT CTTTAACTTT TTCTACTCTG 
    TAAACNGGTG AGAAGAACCA AAACTATAAA CAGGCAAATC TGATTGAATA 
    TAGAGTCTAT GCTTTATTCA TTAAGTTGAG TTGTTCAAAC TTCAAATCAT 
    AGACTCAATA GAACTCTGTT TATATTTGTG TAACTACATA CAAACATTCC 
    TGCATTGATT ATGGCTATAA AGCTATCAGA AAACGTTGTC AGGATCAACT 
    GTTACTATAT CTTGAAACGC AGGTGATAAC TTATTGATTA GAAGGACATA 
    GCGTTTTGCC GGTTCTGGTA AAAGTGTGGA GCAACTTCTG TCAGCCAAGA 
    AGGATTGATG GTGACGACAT TCCGCATGTA TTGACGCTCA GTTGAGACAA 
    TGGATTGGTA AACCACCCAT TTTGGATTCA CCCTGAAGAT TGTATAAAGA 
    GTATATATAC CAAATAAGCT

AT4G18480.1

Model type
Protein coding
Short Description
P-loop containing nucleoside triphosphate hydrolases superfamily protein
Curator Summary
Encodes the CHLI subunit of magnesium chelatase which is required for chlorophyll biosynthesis. All four cysteine residues of the protein form two disulfide bonds (Cys102-Cys193 and Cys354-Cys396) under oxidized conditions but are fully reduced by reduction. It was suggested that the redox state of CHLI is regulated in vivo by the change of the redox environment in the chloroplasts probably via the Trx system.
Computational Description
CHLI1; FUNCTIONS IN: magnesium chelatase activity, ATPase activity; INVOLVED IN: chlorophyll biosynthetic process; LOCATED IN: magnesium chelatase complex, cell wall, chloroplast, chloroplast stroma; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: ATPase, AAA+ type, core (InterPro:IPR003593), Magnesium chelatase, ChlI subunit (InterPro:IPR000523), Magnesium chelatase, ATPase subunit I (InterPro:IPR011775); BEST Arabidopsis thaliana protein match is: magnesium chelatase i2 (TAIR:AT5G45930.1); Has 6584 Blast hits to 6581 proteins in 1594 species: Archae - 309; Bacteria - 5009; Metazoa - 2; Fungi - 0; Plants - 206; Viruses - 0; Other Eukaryotes - 1058 (source: NCBI BLink).
Link
InterPro Scan - TAIR