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Mutant Lines
13-2426-1

13-2426-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

This line has NOT been observed.

Determination of insertion point(s)

5 prime edge [+] show sequence

Chromosome: 3; Position: 4502505 [Map]; Strand: c
- AT3G13730.1 - [+] show detail - cytochrome P450, family 90, subfamily D, polypeptide 1
  Distance: -1585; Region: intergenic
- AT3G13740.1 - [+] show detail - Ribonuclease III family protein
  Distance: -1765; Region: intergenic

TTGACAACAC TATTATAAAG TGAATAAAAA GAGAATGGGA AATTATCTTG 
TTTGGCATTA NTCAAAAGGT TNGATCTATA ATTACAAAAT GAAAAGATTA 
GCTTTTTAGG CTCAATACAA TCAACGATCG CCAAAGTATT CAAGCGGCTA 
AAGAATCTCA CTTCCTTGGA TTCTAATATA TTATACCGCT CATAGTCATA 
CACACACTAT TTAATATGAC ATACGTTTAT ATGAAAGTGA GAAGATGCGC 
TTGTGGTCTA ATCATGAGGA GATTGAATTA GCATTAGATA AGTGTATCAT 
ACTTTTCTTG TAGAGCTTCG TTTCATTACG TAATGTACTC CAAGGTTAAC 
ATTAATGTAT AGAGCCGGTC CTATAATTGA CTCTGTTTAT TTAGGTTGCT 
G

3 prime edge [+] show sequence
1. Chromosome: 3; Position: 4502621 [Map]; Strand: c
  - AT3G13730.1 - [+] show detail - cytochrome P450, family 90, subfamily D, polypeptide 1
    Distance: -1701; Region: intergenic
  - AT3G13740.1 - [+] show detail - Ribonuclease III family protein
    Distance: -1649; Region: intergenic
Flanking Genome Sequence: 13-2426-1 - 3-prime edge
```
GTTGTCAAAA ATATTAATTC TTTTGGGAAA CCTATGATTC TTTTATCATA 
TCTATTAACG CCATTTTTAA GCT
```

AT3G13730.1

Model type: Protein coding
Short Description: cytochrome P450, family 90, subfamily D, polypeptide 1
Curator Summary: Encodes a cytochrome P-450 gene that is involved in brassinosteroid biosynthesis, most likely in the conversion step of teasterone (TE) to 3-dehydroteasterone (3DT), and/or 6-deoxoteasterone (6-deoxoTE) to 6-deoxo-3-dehydroteasterone (6-deoxo3DT); or the conversion of cathasterone (CT) to TE, and/or 6-deoxocathasterone (6-deoxoCT) to 6-deoxoTE. Recently, CYP90D1 was shown to catalyse the C-23 hydroxylation of several brassinosteroids (the enzyme has a broad specificity for 22-hydroxylated substrates). Member of the CYP90C CYP450 family. Similar to Cytochrome P450 90C1 (ROT3).
Computational Description: "cytochrome P450, family 90, subfamily D, polypeptide 1" (CYP90D1); FUNCTIONS IN: oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NADH or NADPH as one donor, and incorporation of one atom of oxygen, oxygen binding; INVOLVED IN: stamen development, petal development, leaf development, brassinosteroid biosynthetic process; LOCATED IN: endomembrane system; EXPRESSED IN: 15 plant structures; EXPRESSED DURING: 8 growth stages; CONTAINS InterPro DOMAIN/s: Cytochrome P450 (InterPro:IPR001128), Cytochrome P450, E-class, group I (InterPro:IPR002401), Cytochrome P450, conserved site (InterPro:IPR017972); BEST Arabidopsis thaliana protein match is: Cytochrome P450 superfamily protein (TAIR:AT4G36380.1); Has 27722 Blast hits to 27661 proteins in 1582 species: Archae - 80; Bacteria - 4717; Metazoa - 10119; Fungi - 4209; Plants - 7293; Viruses - 6; Other Eukaryotes - 1298 (source: NCBI BLink).
Link: InterPro Scan - TAIR

AT3G13740.1

Model type: Protein coding
Short Description: Ribonuclease III family protein
Computational Description: Ribonuclease III family protein; FUNCTIONS IN: RNA binding, ribonuclease III activity; INVOLVED IN: RNA processing; EXPRESSED IN: 21 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Ribonuclease III (InterPro:IPR000999); BEST Arabidopsis thaliana protein match is: Ribonuclease III family protein (TAIR:AT1G55140.1); Has 1542 Blast hits to 1541 proteins in 746 species: Archae - 0; Bacteria - 1385; Metazoa - 0; Fungi - 0; Plants - 63; Viruses - 0; Other Eukaryotes - 94 (source: NCBI BLink).
Link: InterPro Scan - TAIR