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13-3428-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

Specific phenotypes were NOT observed.

Determination of insertion point(s)

  • 5 prime edge [+] show sequence

    1. Chromosome: 1; Position: 13224223 [Map]; Strand: w
      • AT1G35710.1 - [+] show detail - Protein kinase family protein with leucine-rich repeat domain
        Distance: 0; Region: exon2 (CDS)
      • AT1G35720.1 - [+] show detail - annexin 1
        Distance: -974; Region: promoter
    Flanking Genome Sequence: 13-3428-1 - 5-prime edge 
    GGTTTTGATA CTCGAATTGA TAATTGGGAA ACATCCGGGA GATTTGGTAT 
    CGAGTTTGTC TTCGTCTCCA GGAGAAACTC TGTCGCTAAG AAGCATTTCC 
    GATAAACGTG TATTAGAACC ACNGGGGGCA AAATAGAGAG AAGCTCTTGA 
    AGATGGTGGA AATGGCTTTA TTGTGTTTAC AAGCAAATCC AGAATCTCGG 
    CCAACGATGT TGTCAATCTC CACTACATTT TCTTAGAAAC TGAACTTCTC 
    CATCAATTTC TATGTTATCA TTGTGTTTAT CTATACAGCA ACAGTAATCT 
    CTGTTGGCTT TTGTTCTCTG TTCTTGCTTG TGTTTGCAGA AAGGGGTGTC 
    GACTCTCGTT TCAGCTTTTG TTGTTGTTGT TTGGGCTTCT AGGCCTGCTT 
    CAGTTTTGGG CCTTGTTTGT TTTATCCTTT TCGGCCTGCG TGCCATGTTA 
    GGCCTTTTGG TCTTGTAATG TGTTTGGTTA ACGGAAATTT ATGAAGTTAA 
    AAAAAACAAA NAAACAAAAA AAACTGTTAA AAGCATTTTT AGATAAAGGG 
    NCATTGNGTT ACTCCTCACA TATGAACATT CAAATAAAAG TTNTGGATAC 
    TGGCATNTCN TGTATNTCNG CCAGAATTAG TAAGANATTT CTTATGCNTN 
    NTNGGANATT AAAAAAAAAA ATTGCATTGG NNGGAGATTN GNAAAAAANA 
    ANTAAN

  • 3 prime edge [+] show sequence

    1. Chromosome: 1; Position: 13224107 [Map]; Strand: w
      • AT1G35710.1 - [+] show detail - Protein kinase family protein with leucine-rich repeat domain
        Distance: 0; Region: exon2 (CDS)
    Flanking Genome Sequence: 13-3428-1 - 3-prime edge 
    TCAAAACCCC GAAGCTATAC ACATCGCATT TCTCCGTCAC CTTCATCGTG 
    TAAGCAAACT CTGTTGCGAA AAATCAAGTT CAATTTCGAA TTTAGTGATT 
    TAAATTTCAT AAGCTCAAAA TCAAATTTAG GGATTTAGAT TTACCTGGCG 
    CAACGTAGCC GTAGGTTCCA GCAACGGCGG ACCAGTTTGA TGAATCCGTC 
    TTGAGAAACT TCGCTGTACC AAAATCAGAG ATCTTAGCTA TGTAATCGTT 
    ATTGAGAAGG ATGTTGCCGC TGCTAATATC ACGGTGAACG ATCGGAGTTG 
    TTCGGTCATG GTGCATATAC GATAAAGCGT GAGCCACACC TTTCACGACG 
    TTGATCCTTT TGGTCCAAGT GAGTCGCTTA GCTTCTTCGT CGTTGGCTAA 
    GAGTTTGTTT AAACTTCCTT TTTCCATGTA CTCGTAGATC ANAAATGTGT 
    GGCGGCGGTG GGAGCAGAAG CCGAAGAGCT TCACAACGTT GCGGTGACGG 
    ATCTCTGTTA ATGCTTTTAC CTCGTTTAGG AACTCTTGCT TCACAACTGG 
    CTTTGATATC TCTTCGTCTA TTGNGTCATG TANTCGTTTA ACGGCTATGA 
    TTGTATCTTG GAGGTTTGCT CTGTANACTT TGCTGTATCC TCCGGTTCCN 
    ATGAGGNGTG TTGGATNNAA TT

AT1G35710.1

Model type
Protein coding
Short Description
Protein kinase family protein with leucine-rich repeat domain
Computational Description
Protein kinase family protein with leucine-rich repeat domain; FUNCTIONS IN: protein serine/threonine kinase activity, protein tyrosine kinase activity, protein kinase activity, ATP binding; INVOLVED IN: transmembrane receptor protein tyrosine kinase signaling pathway, protein amino acid phosphorylation; LOCATED IN: endomembrane system; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Protein kinase, ATP binding site (InterPro:IPR017441), Serine/threonine-protein kinase domain (InterPro:IPR002290), Leucine-rich repeat-containing N-terminal domain, type 2 (InterPro:IPR013210), Leucine-rich repeat (InterPro:IPR001611), Serine/threonine-protein kinase-like domain (InterPro:IPR017442), Protein kinase-like domain (InterPro:IPR011009), Protein kinase, catalytic domain (InterPro:IPR000719), Leucine-rich repeat, typical subtype (InterPro:IPR003591), Tyrosine-protein kinase, active site (InterPro:IPR008266), Tyrosine-protein kinase, catalytic domain (InterPro:IPR020635); BEST Arabidopsis thaliana protein match is: Leucine-rich repeat receptor-like protein kinase family protein (TAIR:AT4G08850.1); Has 304074 Blast hits to 147448 proteins in 4766 species: Archae - 178; Bacteria - 29187; Metazoa - 106993; Fungi - 12531; Plants - 120860; Viruses - 430; Other Eukaryotes - 33895 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT1G35720.1

Model type
Protein coding
Short Description
annexin 1
Curator Summary
Encodes a member of the annexin gene family, a diverse, multigene family of calcium-dependent, membrane-binding proteins. The protein was determined to have peroxidase activity. This activity is thought to be dependent on the presence of post-translational modifications (most likely phosphorylation). The protein was shown to be present as a mixture of monomer and homodimer. The homodimerization seems to be dependent on the presence of Ca2+ or H2O2. The dimerization was prevented by the addition of DTT, β-mercaptoethanol and TCEP. Annat1 mRNA is expressed in flowers, roots,leaves and stems and is most abundant in stems. mRNA levels are increased in response to oxidative stress. Developmental expression patterns suggest a role in Golgi-mediated polysaccharide secretion.
Computational Description
annexin 1 (ANNAT1); FUNCTIONS IN: in 7 functions; INVOLVED IN: in 8 processes; LOCATED IN: in 10 components; EXPRESSED IN: 31 plant structures; EXPRESSED DURING: 16 growth stages; CONTAINS InterPro DOMAIN/s: Annexin like protein (InterPro:IPR015472), Annexin repeat (InterPro:IPR018502), Annexin repeat, conserved site (InterPro:IPR018252), Annexin (InterPro:IPR001464), Annexin, type plant (InterPro:IPR009118); BEST Arabidopsis thaliana protein match is: annexin 2 (TAIR:AT5G65020.1); Has 3809 Blast hits to 2217 proteins in 205 species: Archae - 0; Bacteria - 0; Metazoa - 2893; Fungi - 160; Plants - 482; Viruses - 0; Other Eukaryotes - 274 (source: NCBI BLink).
Link
InterPro Scan - TAIR