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13-4006-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

This line has NOT been observed.

Determination of insertion point(s)

  • 5 prime edge [+] show sequence

    1. Chromosome: 4; Position: 246744 [Map]; Strand: c
      • AT4G00570.1 - [+] show detail - NAD-dependent malic enzyme 2
        Distance: 0; Region: exon1 (5UTR)
    Flanking Genome Sequence: 13-4006-1 - 5-prime edge 
    GGGATAAACT AACAAAATCG GTTATACGAT AACGGTCGGT ACGGGATTTT 
    CCCATCCTAC TTTCATTAAT ACCTCAGACA CAGCTTCTTC TACTGGAGAA 
    GAGGAGGAGA ACACAAATCC AATTCACACT GGAAACGTAA GAAGAAGCGA 
    TCAAAGCT

  • 3 prime edge [+] show sequence

    1. Chromosome: 4; Position: 246789 [Map]; Strand: c
      • AT4G00570.1 - [+] show detail - NAD-dependent malic enzyme 2
        Distance: -18; Region: promoter
    Flanking Genome Sequence: 13-4006-1 - 3-prime edge 
    GTCTGAGGAA GAGGGAAGAT GTCTTTAAGA ACAGCTGCCC GAAGAATAGT 
    AAAAGGGTCA GACAAATAAA TAAAGCT

AT4G00570.1

Model type
Protein coding
Short Description
NAD-dependent malic enzyme 2
Curator Summary
Encodes an NAD-dependent malic enzyme (NAD-ME) that does not act on oxaloacetate, indicating that it belongs to EC 1.1.1.39. It is a member of the beta family of NAD-MEs in plants. It appears to function as a homodimer or as a heterodimer with the alpha-type NAD-ME2 (At2g13560). NAD-ME2 transcript and protein levels are higher during the night than during the day.
Computational Description
NAD-dependent malic enzyme 2 (NAD-ME2); FUNCTIONS IN: in 7 functions; INVOLVED IN: malate metabolic process; LOCATED IN: mitochondrion, chloroplast; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Malic enzyme, NAD-binding (InterPro:IPR012302), Malic oxidoreductase (InterPro:IPR001891), Malic enzyme, conserved site (InterPro:IPR015884), Malic enzyme, N-terminal (InterPro:IPR012301), NAD(P)-binding domain (InterPro:IPR016040); BEST Arabidopsis thaliana protein match is: NAD-dependent malic enzyme 1 (TAIR:AT2G13560.1); Has 9343 Blast hits to 9326 proteins in 2439 species: Archae - 143; Bacteria - 6323; Metazoa - 607; Fungi - 223; Plants - 463; Viruses - 0; Other Eukaryotes - 1584 (source: NCBI BLink).
Link
InterPro Scan - TAIR