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13-6123-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

This line has NOT been observed.

Determination of insertion point(s)

  • 5 prime edge [+] show sequence

    1. Chromosome: 1; Position: 3080239 [Map]; Strand: w
      • AT1G09530.2 - [+] show detail - phytochrome interacting factor 3
        Distance: 700; Region: intergenic
      • AT1G09540.1 - [+] show detail - myb domain protein 61
        Distance: -5920; Region: intergenic
    Flanking Genome Sequence: 13-6123-1 - 5-prime edge
    GGTGATAAAC TAACAAAATC GGTTATACGA TAACGGTCGG TACGGGATTT 
    TCCCATCCTA CTTTCATCCT ACATAATATA GCTTTAGCAG TAAACTTAAG
    TTCTGTGTGG TAAACCGCGT CTTCACAAAC ATTTTTGCAC CAAAGACTTC
    T
  • 3 prime edge [+] show sequence

    1. Chromosome: 1; Position: 3080229 [Map]; Strand: w
      • AT1G09530.2 - [+] show detail - phytochrome interacting factor 3
        Distance: 690; Region: intergenic
      • AT1G09540.1 - [+] show detail - myb domain protein 61
        Distance: -5930; Region: intergenic
    Flanking Genome Sequence: 13-6123-1 - 3-prime edge
    TTNANCNGTA GAGGTATTTT ACCGACCGTT ACCGACCGTT TTCATCCCTT 
    TGGGGAAACT ATTAATAAAA GTCTGCTCGC TGGCTGGCAG GCTGGGTATA
    TAAAACATCT CTAACTATAG AAACAAGCAT CTACGAGAAG TTGACAACAG
    TTTTGTGAAC ATAAAACAAA GTAACAAACT TCCTAAAGAA TTGAGGAAGA
    AATTTTTTAC TTTCGACAAA ACTCCCAATC GATACAATCA TGGACATATA
    TATAGCTTCC AGCATCCGAG AAACAAATCA GGAGCTCACA TTTGCCCTCT
    TCCAAACTAT GGTTCTCTAG GCTCTAGCTC AACTCCTCCG ATCGTTGACC
    TAACCCTAAC CATCGATTCC CTTTCTTTTT GNTAAACGAA AACGTNACAG
    NCATGCGTGA GCGAATCACT TACTACGTAT AACATAAACT ACATATCNNG
    CTAAGTGATG CGCTNNACAC CGNGTNTCAT GTTACTTATA TACACCCTNT
    ATGTTNCAAC TNAGNAANNA NANANGANGA GNGCNNGCNN AGNNTAGNTN
    TGANNGANGG GGNATNTNNT TTNNNNTNNN TCGGTGNNNG GNGGNNNNNC
    NTCTNNNNNG TNAGAGTGGN NNNCNNGGNA NGNNNNCCCT NTNNNTNNTT
    TGGNAGANNG AGGGGGGGAG AGANNNNGCN TNCNNGCNGN TNGCAGGNGG
    GNTNNATGAN NGTGNANNNC AGNCNNANNN ATANAGAGAN GAANAACNAN
    NNCTTNNNNA AGTGNNGNNC NNAGCAGGGN CGNG

AT1G09530.2

Model type
Protein coding
Short Description
phytochrome interacting factor 3
Curator Summary
Transcription factor interacting with photoreceptors phyA and phyB. Forms a ternary complex in vitro with G-box element of the promoters of LHY, CCA1. Acts as a negative regulator of phyB signalling. It degrades rapidly after irradiation of dark grown seedlings in a process controlled by phytochromes. Does not play a significant role in controlling light input and function of the circadian clockwork. Binds to G- and E-boxes, but not to other ACEs. Binds to anthocyanin biosynthetic genes in a light- and HY5-independent fashion. PIF3 function as a transcriptional activator can be functionally and mechanistically separated from its role in repression of PhyB mediated processes.
Computational Description
phytochrome interacting factor 3 (PIF3); CONTAINS InterPro DOMAIN/s: Helix-loop-helix DNA-binding domain (InterPro:IPR001092), Helix-loop-helix DNA-binding (InterPro:IPR011598); BEST Arabidopsis thaliana protein match is: phytochrome interacting factor 3-like 1 (TAIR:AT2G46970.1); Has 4326 Blast hits to 4310 proteins in 315 species: Archae - 0; Bacteria - 2; Metazoa - 732; Fungi - 183; Plants - 3377; Viruses - 7; Other Eukaryotes - 25 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT1G09540.1

Model type
Protein coding
Short Description
myb domain protein 61
Curator Summary
Encodes putative transcription factor. Mutants lack of mucilage extrusion from the seeds during imbibition. Reduced quantities of mucilage are deposited during the development of the seed coat epidermis in myb61 mutants. Expressed in guard cells,loss of function mutations show an increase in stomatal pore opening suggesting a role in ABA independent regulation of stomatal pore size.
Computational Description
myb domain protein 61 (MYB61); CONTAINS InterPro DOMAIN/s: SANT, DNA-binding (InterPro:IPR001005), Homeodomain-like (InterPro:IPR009057), Myb, DNA-binding (InterPro:IPR014778), HTH transcriptional regulator, Myb-type, DNA-binding (InterPro:IPR017930), Homeodomain-related (InterPro:IPR012287), Myb transcription factor (InterPro:IPR015495); BEST Arabidopsis thaliana protein match is: myb domain protein 50 (TAIR:AT1G57560.1); Has 8976 Blast hits to 8269 proteins in 475 species: Archae - 0; Bacteria - 0; Metazoa - 766; Fungi - 514; Plants - 5880; Viruses - 4; Other Eukaryotes - 1812 (source: NCBI BLink).
Link
InterPro Scan - TAIR