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15-0570-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

Anatomical entity
/structure development stage
Phenotype
vascular leaf (PO:0009025)
  • abnormal (PATO:0000460)
    (Leaves::others)

Determination of insertion point(s)

  • 5 prime edge [+] show sequence

    1. Chromosome: 1; Position: 8122516 [Map]; Strand: c
      • AT1G22940.1 - [+] show detail - thiamin biosynthesis protein, putative
        Distance: 0; Region: exon1 (CDS)
    Flanking Genome Sequence: 15-0570-1 - 5-prime edge
    GACTGCGGTT ATGACGGAAG CGCAATACAC ACCACGAGCT GCGCAGACTT 
    TAAGGTCGGC TTGAATT
  • 3 prime edge [+] show sequence

    1. Chromosome: 1; Position: 8122632 [Map]; Strand: c
      • AT1G22940.1 - [+] show detail - thiamin biosynthesis protein, putative
        Distance: 0; Region: exon1 (CDS)
    Flanking Genome Sequence: 15-0570-1 - 3-prime edge
    CCGCAGTCAC TGCTCAGAAC ACTCGAGGAG TTCAATCTGT TCATCTTCTT 
    CCTCCGGAAT TTATCTCTGA ACAGCTCAAA TCCGTCCTCT CTGACTTCGA
    ATT

AT1G22940.1

Model type
Protein coding
Short Description
thiamin biosynthesis protein, putative
Curator Summary
Encodes a bifunctional enzyme required for thiamine (vitamin B1) biosynthesis. TH1 can phosphorylate HMP-P to produce HMP-PP, the pyrimidine heterocyclic subunit of thiamine. TH1 also catalyzes the condensation of HMP-PP and HET to form thiamine monophosphate (TMP). TH1 also appears capable of phosphorylating HMP based on E.coli mutant complementation assays. th1 mutants are thiamine auxotrophs that die as seedlings on unsupplemented media.
Computational Description
THIAMINE REQUIRING 1 (TH1); FUNCTIONS IN: phosphomethylpyrimidine kinase activity, thiamin-phosphate diphosphorylase activity, hydroxymethylpyrimidine kinase activity; INVOLVED IN: thiamin biosynthetic process; LOCATED IN: chloroplast; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Thiamine monophosphate synthase (InterPro:IPR003733), Phosphomethylpyrimidine kinase type-2 (InterPro:IPR004399), Aldolase-type TIM barrel (InterPro:IPR013785), Phosphomethylpyrimidine kinase type-1 (InterPro:IPR013749); Has 15989 Blast hits to 15891 proteins in 2548 species: Archae - 338; Bacteria - 12149; Metazoa - 165; Fungi - 331; Plants - 107; Viruses - 0; Other Eukaryotes - 2899 (source: NCBI BLink).
Link
InterPro Scan - TAIR