1. Home
  2. Mutant Lines
  3. 15-0660-1

15-0660-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

Specific phenotypes were NOT observed.

Determination of insertion point(s)

  • 5 prime edge [+] show sequence

    1. Chromosome: 1; Position: 9965573 [Map]; Strand: w
      • AT1G28380.1 - [+] show detail - MAC/Perforin domain-containing protein
        Distance: 0; Region: exon7 (CDS)
    Flanking Genome Sequence: 15-0660-1 - 5-prime edge 
    ATAAACTAAC AAAATCGGTT ATACGATAAC GGTCGGTACG GGATTTTCCC 
    ATCCTACTTT CATCCACGTT TGCACCTACC CAGTTCAGTA CAACGGAGCA 
    CGGTCTGATG ACACCGCCTC TATTGTAACC AAAGCCTGGT TAGAAGTGAA 
    AGGAATGGGG ATGAGGAAAG TTCTCTTCTT GAGGCTCGGT TTCTCACTAG 
    ATGCTTCAGC TGTTACACGT AAATCGTGCT GGGACAATCT TTCAACAAAC 
    TCGCGCAAAT CCGGTGTTTT CTCGATGATC AGTACAAGAC TAAGCACGGG 
    ATTAAGTCCA AACCCGGCAA CAACTAAGCC ACAGTCTAAG ATTGATATAA 
    ACTCTGCAGT GTATCCTAGA GGGCCATCAC CGCCTGTGAA ACCAAAGTTG 
    CTGAGTCTTG TAGACACGAA GGAAGTGATG AGAGGTCCAG AGGAACAACC 
    TGGATATTGG GTTGTGACTG GTGCTAAACT TTGTGTTGAG GCAGGCAAGA 
    TCTCAATCAA AGCTAAATAC TCTCTGCTCA CTGTCATATC GGAAGACTCA 
    CTGGTCTGAG AGATTCATTT ATTCAGTTCC AAATGACAGG GAGGGGTGTC 
    TAAGTAGAGA ACACAAGTGT AGTGTGCGTG TTGGATTTTG ACAGATTTCA 
    GTTTGTGATT AGCTTTAATC AGTTGTTTTT GGGTTTGTGA ACAAAATTAT 
    TTATCTGGTT GATGAAATGG TTNCGTTGGA TATGANANTG GTGAATTTTA 
    CCATATGTAT ATATG

  • 3 prime edge [+] show sequence

    1. Chromosome: 1; Position: 9965560 [Map]; Strand: w
      • AT1G28380.1 - [+] show detail - MAC/Perforin domain-containing protein
        Distance: 0; Region: exon7 (CDS)
    Flanking Genome Sequence: 15-0660-1 - 3-prime edge 
    GAAAACGGTA GAGGTATTTT ACCGACCGTT ACCGACCGTT TTCATCCCAA 
    GAATATTCCC CATTTCACAG GTACATAATA CCCTTTTTCA ACTGGTTCCT 
    CGATGGGTTC ATAGGTGTCA TCGTGTGAGA GATGGAGACT TGGAGGACAT 
    GCAGAGAGGT GCTGCAAATG TATTGCTAGA TGATTTCCTT TCTTTCCCTC 
    AAGGAAGAAG CGTAGTCCGG TTACNTGGTC GCTCACCTGA ATCAACCTAC 
    ACAACAGTTT TCAAAAAATT GGAAAGTGTA AACTTAGCAA AGGGTTATCA 
    TTTACATGGA TCAATGTGAA AAACAAATGT ACCTTTGATG TGTTGACGTA 
    TAGTTTTGGA CCCATCAAAG AGAACTGAAG GGATGGAGAA CTTTGTTTCG 
    AGCGGCGTAG TCCAAGAGGG AGATCTCCAT ACACTGGAGC CCATTGGCGT 
    GGAAGTTGAA ACTCAAGAAA CTGATGCAAC TCTTCTATAG GTGGCTTATC 
    TACAACATTT TAAACAACCT TGTTACCTGA ATGAACCGTT GAGTACCTAA 
    GATACAAAAG CCAAAAAAGA ACAAAATACT CACATCGGAG GTATAAGTTC 
    ACTGCATGAC TAAGAAATCC TGTTCCTGGG AGACCGCTCA AGAGTGAAGT 
    TNTAGGGAAC AAAACACATC GATATAACGG TTGGGTGCTT GGTGATACTG 
    NTTGAGAGCC ATCGTTCATG ACTTTGCCCC ANATCAACAC CTCCTTTTNT 
    GATACAAACC CTCATAATT

AT1G28380.1

Model type
Protein coding
Short Description
MAC/Perforin domain-containing protein
Curator Summary
This gene is predicted to encode a protein involved in negatively regulating salicylic acid-related defense responses and cell death programs. nsl1 mutants develop necrotic lesions spontaneously and show other features of a defense response, such as higher levels of SA and disease resistance-related transcripts, in the absence of a biotic stimulus. The NSL1 protein is predicted to have a MACPF domain, found in proteins that form a transmembrane pore in mammalian immune responses. NSL1 transcript levels do not appear to change in response to biotic stresses, but are elevated by cycloheximide in seedlings, and by sodium chloride in roots.
Computational Description
necrotic spotted lesions 1 (NSL1); CONTAINS InterPro DOMAIN/s: Membrane attack complex component/perforin (MACPF) domain (InterPro:IPR020864); BEST Arabidopsis thaliana protein match is: MAC/Perforin domain-containing protein (TAIR:AT4G24290.2); Has 203 Blast hits to 202 proteins in 18 species: Archae - 0; Bacteria - 0; Metazoa - 1; Fungi - 0; Plants - 202; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink).
Link
InterPro Scan - TAIR