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Mutant Lines
15-0683-1

15-0683-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

Specific phenotypes were NOT observed.

Determination of insertion point(s)

5 prime edge [+] show sequence

Chromosome: 1; Position: 7645956 [Map]; Strand: w
- AT1G21750.1 - [+] show detail - PDI-like 1-1
  Distance: 0; Region: intron1 (CDS)
- AT1G21750.2 - [+] show detail - PDI-like 1-1
  Distance: 0; Region: intron1 (CDS)

TCACCGATAC CATCAACAAA CACGATTTCA TCGTCGTCGA GTTCTACGCC 
CCATGGTATT CAAATTTTGT GACTTTTCAA TCTTTTTACT ATTTCTGTTG 
ACATGGTTTT TTTTTGGTAA AAAATTGACA TGGGTTTTGN GTCGNGTTCG 
TGAATCTGGG GGATCTGAAT CTATCATTTG NGTTTTNGGA TTTGCATGCC 
AANGATTTCT TATTCTCTCG CTNGNGNGAT TTGAATTGGC GTAGCTGATC 
TGTTTTACTA ATCANAACTT TNGTTGCAGT TTTGTAATTT GTAACNATTT 
GATCTATTCC GATCTATGTT ACTATGTAAC TGAGTNGTCT GATTTGAATA 
GNGGTCTCAT TTTAATTTTA CTCTGTAATN GATTTGGGAA AGTATTTGAT 
CTAAATCTTC ATCATTTGGT AATCCGNATT ATNGCCCATC TTNAGGNGTG 
GACACTGCAA GCAGCTNGCT CCTGNGGNAC TATGACTTTT TTCAATCACT 
TAAAATNGGA TTGGCAGGNG GAGTTTTTTT TAAACNGGGN CAAATAAAAG 
NNNNNAAAGG GTNAGGGGGG GGNNTNAATT TNNTGGNNNN AANNGGGNNA 
AGGGGGGGNN AGANTTTNNG NCNNAAANGC CCCCCCCNGG GGNNNTGNNN 
NNAAANNNNN CCNNNGNNGG NANNAAACNA AANAN

3 prime edge [+] show sequence
1. Chromosome: 1; Position: 7645840 [Map]; Strand: w
  - AT1G21750.1 - [+] show detail - PDI-like 1-1
    Distance: 0; Region: exon1 (CDS)
  - AT1G21750.2 - [+] show detail - PDI-like 1-1
    Distance: 0; Region: exon1 (CDS)
Flanking Genome Sequence: 15-0683-1 - 3-prime edge
```
ATCGGTGAAG TTAGTGTGAT CCAAGGTCAA CACGGAACTC CTTCGTCTCC 
GTCTCTTCGC TTCTGATAGA CGAAGCGCAC AAAGACAACA CAAGGATCGA 
AAACAACGTG AAGCCCCTCA TCGCCATTAT CGCTCTCTCT TTCTCTCTAA 
CAATGTTTAG CTTGAATT
```

AT1G21750.1

Model type: Protein coding
Short Description: PDI-like 1-1
Curator Summary: Encodes a protein disulfide isomerase-like (PDIL) protein, a member of a multigene family within the thioredoxin (TRX) superfamily; isoform contains non-consensus GA donor splice site at intron 9. Transcript levels for this gene are up-regulated in response to three different chemical inducers of ER stress (dithiothreitol, beta-mercaptoethanol, and tunicamycin). Neither AtIRE1-2 nor AtbZIP60 appear to be required for this response.
Computational Description: PDI-like 1-1 (PDIL1-1); FUNCTIONS IN: protein disulfide isomerase activity; INVOLVED IN: in 6 processes; LOCATED IN: in 8 components; EXPRESSED IN: 29 plant structures; EXPRESSED DURING: 16 growth stages; CONTAINS InterPro DOMAIN/s: Disulphide isomerase (InterPro:IPR005788), Thioredoxin fold (InterPro:IPR012335), Thioredoxin domain (InterPro:IPR013766), Thioredoxin, conserved site (InterPro:IPR017937), Thioredoxin-like subdomain (InterPro:IPR006662), Protein disulphide isomerase (InterPro:IPR005792), Thioredoxin-like (InterPro:IPR017936), Thioredoxin-like fold (InterPro:IPR012336); BEST Arabidopsis thaliana protein match is: PDI-like 1-2 (TAIR:AT1G77510.1); Has 31573 Blast hits to 17834 proteins in 2924 species: Archae - 337; Bacteria - 15428; Metazoa - 5451; Fungi - 1644; Plants - 2422; Viruses - 29; Other Eukaryotes - 6262 (source: NCBI BLink).
Link: InterPro Scan - TAIR

AT1G21750.2

Model type: Protein coding
Short Description: PDI-like 1-1
Curator Summary: Encodes a protein disulfide isomerase-like (PDIL) protein, a member of a multigene family within the thioredoxin (TRX) superfamily; isoform contains non-consensus GA donor splice site at intron 9. Transcript levels for this gene are up-regulated in response to three different chemical inducers of ER stress (dithiothreitol, beta-mercaptoethanol, and tunicamycin). Neither AtIRE1-2 nor AtbZIP60 appear to be required for this response.
Computational Description: PDI-like 1-1 (PDIL1-1); FUNCTIONS IN: protein disulfide isomerase activity; INVOLVED IN: in 6 processes; LOCATED IN: in 8 components; EXPRESSED IN: 29 plant structures; EXPRESSED DURING: 16 growth stages; CONTAINS InterPro DOMAIN/s: Thioredoxin fold (InterPro:IPR012335), Disulphide isomerase (InterPro:IPR005788), Thioredoxin domain (InterPro:IPR013766), Thioredoxin, conserved site (InterPro:IPR017937), Thioredoxin-like subdomain (InterPro:IPR006662), Protein disulphide isomerase (InterPro:IPR005792), Thioredoxin-like (InterPro:IPR017936), Thioredoxin-like fold (InterPro:IPR012336); BEST Arabidopsis thaliana protein match is: PDI-like 1-2 (TAIR:AT1G77510.1); Has 30765 Blast hits to 17459 proteins in 2902 species: Archae - 337; Bacteria - 14753; Metazoa - 5498; Fungi - 1629; Plants - 2433; Viruses - 29; Other Eukaryotes - 6086 (source: NCBI BLink).
Link: InterPro Scan - TAIR