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15-0722-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

This line has NOT been observed.

Determination of insertion point(s)

  • 5 prime edge [+] show sequence

    1. Chromosome: 4; Position: 247293 [Map]; Strand: w
      • AT4G00570.1 - [+] show detail - NAD-dependent malic enzyme 2
        Distance: -522; Region: promoter
    Flanking Genome Sequence: 15-0722-1 - 5-prime edge 
    CGNGATAAAC TAACAAAATC GGTTATACGA TAACGGTCGG TACGGGATTT 
    TCCCATCCTA CTTTCATATT CATGTCAAGA TTTCGAATCC TATAAATTTG 
    ATCACAGCAA AATTAAACAT AGTTAAGGGC AAGGGAAAAA AAAAANAANG 
    CANGCNTTNA TTNATTNATT TNGNCNTAAA NAAANCNTTC AANANGNGTN 
    AGTNTTTCNT AATCAAATCC TNCTANCAAA TANCTATAAT CTNAATNGNC 
    TTTNGNCGGC CAATAANCTC NANCCAAAAT TNTTNGNCNA NCCAAATNGG 
    TAAAAAANAA NCTCNANTAA AAATNCNTNT CCNATTTGAA AAATNTTNCG 
    GTCCGTAGNC NGANCTNTAA NCAAAAATNT NAGTNTTTTT NTTTTNCNTN 
    GAAAAANATC TNGNCTNNGG TNAATTNTNT TTTGGGAAAT ANANAAATCT 
    NTNTNNGTNN GGTNCTNAGG GTTTAAANAA ANAAANNTTG GGGGTTTNTA 
    ACTCCNTNAG CANCTTTTTN ANNGGCCCAG GTAATNCTNT AANNCNCTCN 
    GANGTTGGAN CTTAAANCCN GTNCANGTCC NTGAAATAAA ACTNTGTNNN 
    ACTTTGTTGG CCTGGCCNAC TTTCTTGGNN CCTTAAACAT TGAAAACATC 
    TGGGACTTAT NTTCAACATC TCCTCACNGT TTGGAATTTN TTAAACCTTG 
    CAGCAANGCN NCTAAACAAG NAACAATCTT TNNAC

AT4G00570.1

Model type
Protein coding
Short Description
NAD-dependent malic enzyme 2
Curator Summary
Encodes an NAD-dependent malic enzyme (NAD-ME) that does not act on oxaloacetate, indicating that it belongs to EC 1.1.1.39. It is a member of the beta family of NAD-MEs in plants. It appears to function as a homodimer or as a heterodimer with the alpha-type NAD-ME2 (At2g13560). NAD-ME2 transcript and protein levels are higher during the night than during the day.
Computational Description
NAD-dependent malic enzyme 2 (NAD-ME2); FUNCTIONS IN: in 7 functions; INVOLVED IN: malate metabolic process; LOCATED IN: mitochondrion, chloroplast; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Malic enzyme, NAD-binding (InterPro:IPR012302), Malic oxidoreductase (InterPro:IPR001891), Malic enzyme, conserved site (InterPro:IPR015884), Malic enzyme, N-terminal (InterPro:IPR012301), NAD(P)-binding domain (InterPro:IPR016040); BEST Arabidopsis thaliana protein match is: NAD-dependent malic enzyme 1 (TAIR:AT2G13560.1); Has 9343 Blast hits to 9326 proteins in 2439 species: Archae - 143; Bacteria - 6323; Metazoa - 607; Fungi - 223; Plants - 463; Viruses - 0; Other Eukaryotes - 1584 (source: NCBI BLink).
Link
InterPro Scan - TAIR