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15-1210-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

Specific phenotypes were NOT observed.

Determination of insertion point(s)

  • 5 prime edge [+] show sequence

    1. Chromosome: 1; Position: 8182990 [Map]; Strand: c
      • AT1G23080.1 - [+] show detail - Auxin efflux carrier family protein
        Distance: 0; Region: exon1 (CDS)
      • AT1G23080.3 - [+] show detail - Auxin efflux carrier family protein
        Distance: 0; Region: exon1 (CDS)
      • AT1G23080.2 - [+] show detail - Auxin efflux carrier family protein
        Distance: 0; Region: exon1 (CDS)
    Flanking Genome Sequence: 15-1210-1 - 5-prime edge
    ATGGGAATAC CTCTCTTGAT CGCCATGTAC GGAGAATATT CCGGCTCACT 
    CATGGTTCAG ATCGTTGTTC TTCAGTGTAT AATCTGGTAC ACGCTTCTTC
    TCTTTCTTTT CGAATACAGA GGAGCTAAGA TCTTGATCAT GGAACAGTTT
    CCAGAGACTG GTGCTTCGAT TGTATCATTC AAAGTCGAGT CCGATGTTGT
    TTCTTTAGAT GGACATGATT TTCTTGAAAC TGATGCTCAA ATCGGTGACG
    ATGGTAAGCT
  • 3 prime edge [+] show sequence

    1. Chromosome: 1; Position: 8183106 [Map]; Strand: c
      • AT1G23080.1 - [+] show detail - Auxin efflux carrier family protein
        Distance: 0; Region: exon1 (CDS)
      • AT1G23080.3 - [+] show detail - Auxin efflux carrier family protein
        Distance: 0; Region: exon1 (CDS)
      • AT1G23080.2 - [+] show detail - Auxin efflux carrier family protein
        Distance: 0; Region: exon1 (CDS)
    Flanking Genome Sequence: 15-1210-1 - 3-prime edge
    ATTCCCATAA CGAGAGTGTT GGGAAGTGTT GAAAGAGAGA AGATTGTGAT 
    GCTCCATTCA AGACTACCGG AGCGAGTGAA GTTAGCCCAG ATGATTAGGA
    GAGTGAGCAT GATTAGTTTT TGGAGTGTAT CNGGCTGCGA TGAATCGGAG
    ATTCATTGCG TAAGGATTGT TTGAGGAGAT GAAGTGGAAA GAGAGAAGAG
    GGACGGCGAA AATAGCGACG AAGCGGTTTA TGCCGGAACA TTGGTCTGGT
    GAGAAGATTT TCCACCACCG GACAGAGCCG TAAGCGAGGA TCATGGCTAC
    GTAGAGTGGT ATCACCGCCG TGAGGACGGT GTAGAGGTCG TGCCATGTGA
    TCATATTGTT GTTCGCCGGA GTGGCAAAAG TGTAATTTTG AGTGTGGAAA
    CCGTTAGGAA GTGAGTTAGT GTCAGTCTCT GTTCTCTATG TGATGACGGA
    AGCT

AT1G23080.1

Model type
Protein coding
Short Description
Auxin efflux carrier family protein
Curator Summary
Encodes a novel component of auxin efflux that is located apically in the basal cell and is involved during embryogenesis in setting up the apical-basal axis in the embryo. It is also involved in pattern specification during root development. In roots, it is expressed at lateral and basal membranes of provascular cells in the meristem and elongation zone, whereas in the columella cells it coincides with the PIN3 domain. Plasma membrane-localized PIN proteins mediate a saturable efflux of auxin. PINs mediate auxin efflux from mammalian and yeast cells without needing additional plant-specific factors. The action of PINs in auxin efflux is distinct from PGPs, rate-limiting, specific to auxins and sensitive to auxin transport inhibitors. PINs are directly involved of in catalyzing cellular auxin efflux.
Computational Description
PIN-FORMED 7 (PIN7); FUNCTIONS IN: auxin:hydrogen symporter activity, transporter activity, auxin efflux transmembrane transporter activity; INVOLVED IN: response to cyclopentenone, root development, pattern specification process, auxin polar transport, longitudinal axis specification; LOCATED IN: plasma membrane, membrane; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Auxin efflux carrier, subgroup (InterPro:IPR014024), Auxin efflux carrier (InterPro:IPR004776); BEST Arabidopsis thaliana protein match is: Auxin efflux carrier family protein (TAIR:AT1G70940.1); Has 1748 Blast hits to 1583 proteins in 451 species: Archae - 37; Bacteria - 919; Metazoa - 14; Fungi - 2; Plants - 486; Viruses - 0; Other Eukaryotes - 290 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT1G23080.2

Model type
Protein coding
Short Description
Auxin efflux carrier family protein
Curator Summary
Encodes a novel component of auxin efflux that is located apically in the basal cell and is involved during embryogenesis in setting up the apical-basal axis in the embryo. It is also involved in pattern specification during root development. In roots, it is expressed at lateral and basal membranes of provascular cells in the meristem and elongation zone, whereas in the columella cells it coincides with the PIN3 domain. Plasma membrane-localized PIN proteins mediate a saturable efflux of auxin. PINs mediate auxin efflux from mammalian and yeast cells without needing additional plant-specific factors. The action of PINs in auxin efflux is distinct from PGPs, rate-limiting, specific to auxins and sensitive to auxin transport inhibitors. PINs are directly involved of in catalyzing cellular auxin efflux.
Computational Description
PIN-FORMED 7 (PIN7); FUNCTIONS IN: auxin:hydrogen symporter activity, transporter activity, auxin efflux transmembrane transporter activity; INVOLVED IN: response to cyclopentenone, root development, pattern specification process, auxin polar transport, longitudinal axis specification; LOCATED IN: plasma membrane, membrane; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Auxin efflux carrier, subgroup (InterPro:IPR014024), Auxin efflux carrier (InterPro:IPR004776); BEST Arabidopsis thaliana protein match is: Auxin efflux carrier family protein (TAIR:AT1G70940.1); Has 700 Blast hits to 605 proteins in 126 species: Archae - 0; Bacteria - 174; Metazoa - 8; Fungi - 2; Plants - 457; Viruses - 0; Other Eukaryotes - 59 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT1G23080.3

Model type
Protein coding
Short Description
Auxin efflux carrier family protein
Curator Summary
Encodes a novel component of auxin efflux that is located apically in the basal cell and is involved during embryogenesis in setting up the apical-basal axis in the embryo. It is also involved in pattern specification during root development. In roots, it is expressed at lateral and basal membranes of provascular cells in the meristem and elongation zone, whereas in the columella cells it coincides with the PIN3 domain. Plasma membrane-localized PIN proteins mediate a saturable efflux of auxin. PINs mediate auxin efflux from mammalian and yeast cells without needing additional plant-specific factors. The action of PINs in auxin efflux is distinct from PGPs, rate-limiting, specific to auxins and sensitive to auxin transport inhibitors. PINs are directly involved of in catalyzing cellular auxin efflux.
Computational Description
PIN-FORMED 7 (PIN7); FUNCTIONS IN: auxin:hydrogen symporter activity, transporter activity, auxin efflux transmembrane transporter activity; INVOLVED IN: response to cyclopentenone, root development, pattern specification process, auxin polar transport, longitudinal axis specification; LOCATED IN: plasma membrane, membrane; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Auxin efflux carrier, subgroup (InterPro:IPR014024), Auxin efflux carrier (InterPro:IPR004776); BEST Arabidopsis thaliana protein match is: Auxin efflux carrier family protein (TAIR:AT1G70940.1); Has 1749 Blast hits to 1582 proteins in 451 species: Archae - 37; Bacteria - 920; Metazoa - 12; Fungi - 2; Plants - 486; Viruses - 0; Other Eukaryotes - 292 (source: NCBI BLink).
Link
InterPro Scan - TAIR