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15-2075-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

Anatomical entity
/structure development stage		 Phenotype
whole plant flowering stage (PO:0007016)
  • premature (PATO:0000694)
    (Flowering & Growth::early flowering)
vascular leaf (PO:0009025)
  • abnormal (PATO:0000460)
    (Leaves::others)
sporophyte vegetative stage (PO:0007134)
  • abnormal (PATO:0000460)
    (Flowering & Growth::growth defective)
seedling (PO:0008037)
  • abnormal (PATO:0000460)
    (Seedling::others)

Determination of insertion point(s)

  • 3 prime edge [+] show sequence

    1. Chromosome: 1; Position: 9963875 [Map]; Strand: c
      • AT1G28380.1 - [+] show detail - MAC/Perforin domain-containing protein
        Distance: 0; Region: exon1 (CDS)
    Flanking Genome Sequence: 15-2075-1 - 3-prime edge 
    GTTCCAGGTT AGTCGAAATC GACCCGACTC GGAACCGTGA CTTAATCTTC 
    CCCGGTGGTA TCGTCGTTAA CAACGTCTCG AGCTCGATTA AGTGCGATAA 
    AGGCGAACGT ACTCGATTAC GCTCCGATAT ACTCTCTTTT AATCAGGTTT 
    TTTAGTTAAA ATCTAAATAA TCCACTGAGA TTCGTGTTTT TCGTTTTTAG 
    AAACCCTAAT TTTTTAGTGT GTTTTTTTTG TTTTTGTGTA GATGTCGGAG 
    AAGTTTAATC AAGATATGTG TCTCTCCGGG AAGATTCCGT CAGGGATGTT 
    TAATAATATG TTTGCGTTTA GTAAATGTTG GCCAAAAGAT GCGAGCTCTG 
    TTAAGACTTT AGCTTATGAT GGTTGGTTTA TTAGTTTGTA TAGTGTAGAG 
    ATTGTTAGGA AGCAGTTGAC ACTACGTGAT GAAGTGAAAC GTGAGGTTCC 
    TTCTTCTTGG GACTCTGCTG CACTTGCTGG GTATGGTTTT GATGATTCAC 
    TTANAAAGTT TTTAAGTGTG ATTTGGAGTT TATGTTAATG AGTGAGATTG 
    AATTTTTTGT TTTTTTTATA GGNTTATTGA GAAATATGGT ACTCATGTTG 
    TTGTTGGAGG GACTNTGGGG AGGTAAAGAN GGTCNTTCNT GTNAAANANA 
    TGCGTAATTN NAAACATNAA CCTTGGGGAA TTNNNNAAAA ATGTTGAANC 
    TTTGGGGGNG ANGAAAAATT TTGGGN

AT1G28380.1

Model type
Protein coding
Short Description
MAC/Perforin domain-containing protein
Curator Summary
This gene is predicted to encode a protein involved in negatively regulating salicylic acid-related defense responses and cell death programs. nsl1 mutants develop necrotic lesions spontaneously and show other features of a defense response, such as higher levels of SA and disease resistance-related transcripts, in the absence of a biotic stimulus. The NSL1 protein is predicted to have a MACPF domain, found in proteins that form a transmembrane pore in mammalian immune responses. NSL1 transcript levels do not appear to change in response to biotic stresses, but are elevated by cycloheximide in seedlings, and by sodium chloride in roots.
Computational Description
necrotic spotted lesions 1 (NSL1); CONTAINS InterPro DOMAIN/s: Membrane attack complex component/perforin (MACPF) domain (InterPro:IPR020864); BEST Arabidopsis thaliana protein match is: MAC/Perforin domain-containing protein (TAIR:AT4G24290.2); Has 203 Blast hits to 202 proteins in 18 species: Archae - 0; Bacteria - 0; Metazoa - 1; Fungi - 0; Plants - 202; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink).
Link
InterPro Scan - TAIR