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15-4124-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

Specific phenotypes were NOT observed.

Determination of insertion point(s)

  • 3 prime edge [+] show sequence

    1. Chromosome: 1; Position: 2689069 [Map]; Strand: c
      • AT1G08490.1 - [+] show detail - chloroplastic NIFS-like cysteine desulfurase
        Distance: -474; Region: promoter
      • AT1G08500.1 - [+] show detail - early nodulin-like protein 18
        Distance: 0; Region: exon1 (5UTR)
    Flanking Genome Sequence: 15-4124-1 - 3-prime edge 
    CTTTTCGAGC GACGAGGTAT CCTACTNNGC CNGACCAACC CGCCATNCNC 
    AATGTNTACT CTAGACNAAC GGACANGAGA GTNNGAAGAG NCNTGTCCGC 
    CTTCTTGTTC ATCTTGTGTC AATGTGTTAC TCATCATGTG CCTTATGCTT 
    CTGAGTTTAT CAGCTGATGC GTACAAGAAC TACACTGTGG GAGAGTCCAC 
    TGGGATGGGT CGATNTTCAA GAGANACCCT NTGCTAATTA TNANNAAANG 
    GGCCGATTCC ANATCCTTTT CTTTGGGTGA TTANCTCAGT AAGATTTTTT 
    TCTTNAACCC CATTTTCGTT ACTACNATAA CNTTANCAAA ATGTNNTATC 
    NGTTTGNNTT GTTNGCAATA TGTANTATNC AACNCANATA NCAACCATTC 
    NGTNGTTTAT ACATATGATN TCAAAACATA CAAAGATCNG NGATTACGAC 
    AACAATGANA ACAATGATAC AACAGAGTGG NCGGCNTGCA AANCCTTCAG 
    CAACATCTCC TGTTCNNGTA TAAATAAGTG TNNCTCTNGT TAAAGANGGT 
    TNCAATTATT NCTNCTCGGG AAACTNTNGA TGGCGNACAA TGTAAATTTC 
    GGGCAGCATT NNATGATCAA TGGTAACACA TGGTCCGGGC TTACCACNAT 
    TCATNATCAC CAANATNATG CTGNTGCACC CTGGACCTAC CGAATCCCCT 
    TAANTCCGGN GANAGANTNA ANCNNCTCCT NGATACCANT CGTCNNGCCC 
    AATTTTCNNT NNTCCNTAAN ATTTNGANAG NNCATNANAA AANTAAAAAG 
    GTTCCATANT

  • 5 prime edge [+] show sequence

    1. Chromosome: 1; Position: 2689040 [Map]; Strand: c
      • AT1G08490.1 - [+] show detail - chloroplastic NIFS-like cysteine desulfurase
        Distance: -445; Region: promoter
      • AT1G08500.1 - [+] show detail - early nodulin-like protein 18
        Distance: 0; Region: exon1 (5UTR)
    Flanking Genome Sequence: 15-4124-1 - 5-prime edge 
    GATAAACTAA CAAAATCGGT TATACGATAA CGGTCGGTAC GGGATTTTCC 
    CATCCTACTT TCATCCTTTG TAATAAAGAA AGGCATAAGA AACTGTACAT 
    AAAGTAATAA TGAGCTCGTC ATTGACTGCA TATAAAACCA AGACATGTGT 
    GAGTGTGACA TGTGGACAAT TCTTGGGGAT GCGAAGGAAC ATGAGACGAT 
    ACGAAGTGGT CGGTCCAATC AAATGACAAC GCCTCTTTGC TCTTCCCCAT 
    GTGATCTCTC ACTCTCACAC CTTACTTTTG CTGTCTTCGC TTCCCTACGT 
    AACATTACTT CTTTCATAAA TATCTGTCAA CTGCCTTTTT CTACTAAATG 
    GAGGATATTT TAGATAACGT GTTTTGTAGT GTTTCAAAGT CGGTTGAACC 
    GGTATTTAAC CGGTTTGATT GGACCTTAAA AACCAAGGGC CCATTTAATG 
    GAGTAAAAGG CCTAATTCGG CCCATATCTT TAGATGAAAT TAATCTTTTG 
    ACCGCCGCCT GTGGTCGAAC TTGTGACTTT GAAGACTCAC TCTTGTTCAT 
    TCGTCTTCTC CACCATGGAA GGTGTGGCTA TGAAACTCCC GTCGTTCCCT 
    AACGCCATCT CCATCGGCCA CCGGAGCTTC TCCCGCGTTC GTTGCTCCTC 
    TTCGCTATCC GTTTGTTCCG CCGCCGCCGC TTCCTCCGCC ACCATCTCCA 
    CTGATTCCGA ATCCGTCTCT CTCGGCCATC GCGTTCGAAA AGATTTCCGA 
    ATT

AT1G08490.1

Model type
Protein coding
Short Description
chloroplastic NIFS-like cysteine desulfurase
Curator Summary
Chloroplastic NifS-like protein that can catalyze the conversion of cysteine into alanine and elemental sulfur (S(0)) and of selenocysteine into alanine and elemental Se (Se(0)). Overexpression enhances selenium tolerance and accumulation.
Computational Description
chloroplastic NIFS-like cysteine desulfurase (CPNIFS); FUNCTIONS IN: transaminase activity, selenocysteine lyase activity, cysteine desulfurase activity; INVOLVED IN: iron incorporation into metallo-sulfur cluster, response to selenium ion, sulfur metabolic process, selenium metabolic process; LOCATED IN: chloroplast; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Pyridoxal phosphate-dependent transferase, major domain (InterPro:IPR015424), Aminotransferase, class V/Cysteine desulfurase (InterPro:IPR000192), Cysteine desulfurase, SufS (InterPro:IPR010970), Pyridoxal phosphate-dependent transferase, major region, subdomain 1 (InterPro:IPR015421); BEST Arabidopsis thaliana protein match is: nitrogen fixation S (NIFS)-like 1 (TAIR:AT5G65720.1); Has 22660 Blast hits to 22653 proteins in 2897 species: Archae - 350; Bacteria - 15408; Metazoa - 379; Fungi - 404; Plants - 268; Viruses - 1; Other Eukaryotes - 5850 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT1G08500.1

Model type
Protein coding
Short Description
early nodulin-like protein 18
Computational Description
early nodulin-like protein 18 (ENODL18); FUNCTIONS IN: electron carrier activity, copper ion binding; LOCATED IN: anchored to membrane; EXPRESSED IN: 14 plant structures; EXPRESSED DURING: LP.06 six leaves visible, 4 anthesis, petal differentiation and expansion stage, LP.08 eight leaves visible, E expanded cotyledon stage; CONTAINS InterPro DOMAIN/s: Plastocyanin-like (InterPro:IPR003245), Cupredoxin (InterPro:IPR008972); BEST Arabidopsis thaliana protein match is: Cupredoxin superfamily protein (TAIR:AT2G31050.1); Has 799 Blast hits to 788 proteins in 52 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 799; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink).
Link
InterPro Scan - TAIR