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16-0768-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

Specific phenotypes were NOT observed.

Determination of insertion point(s)

  • 5 prime edge [+] show sequence

    1. Chromosome: 3; Position: 23452932 [Map]; Strand: w
      • AT3G63520.1 - [+] show detail - carotenoid cleavage dioxygenase 1
        Distance: 0; Region: exon1 (5UTR)
    Flanking Genome Sequence: 16-0768-1 - 5-prime edge 
    GGGATAAACT AACAAAATCG GTTATACGAT AACGGTCGGT ACGGGATTTT 
    CCCATCCTAC TTTCATCCCT CAAACAATCA TGGCGGAGAA ACTCAGTGAT 
    GGCAGCATCA TCATCTCAGT CCATCCTAGA CCCTCCAAGG GTTTCTCCTC 
    GAAGCT

  • 3 prime edge [+] show sequence

    1. Chromosome: 3; Position: 23452922 [Map]; Strand: w
      • AT3G63520.1 - [+] show detail - carotenoid cleavage dioxygenase 1
        Distance: 0; Region: exon1 (5UTR)
    Flanking Genome Sequence: 16-0768-1 - 3-prime edge 
    ATGAAAACGG TAGAGGTATT TTACCGACCG TTACCGACCG TTTTCATCCC 
    TGCAAGGAGG AGGTGATTTG AGACCAGATA ATATGGCCCG CGTCTTGGCG 
    GAAACGCTGC CACGTCGCAA CGGAATCTTT TTTCTTGAGA TGATGGACCC 
    AGTTATATTT CCGGCCCAAA CAAAAAAAAT AACAAAAAAG CCCATACACN 
    GAGCCCAAGA CTAACAACCA CTTGAAGTAA GCCCCGTTCA ATTTACAATT 
    TTAATCGACA GAGACAATCA CAAACACAGA TTACAAACGA CAACTAAGCG 
    CATAAGATGA TTTCTTCTCC AGCTTCGTAT GGTGGGGGCA ATACATTGTG 
    CACATCTGCN AACACATCTT CGTCTCCGCT CTCTGGACTC CCAGATATGG 
    GTGAATCTAA NACACAGTCC GGGATCGCCC TCAAGCT

AT3G63520.1

Model type
Protein coding
Short Description
carotenoid cleavage dioxygenase 1
Curator Summary
Encodes a protein with 9-cis-epoxycarotenoid dioxygenase activity. The enzyme was shown to act on a variety of carotenoid including β-carotene, lutein, zeaxanthin, and all-trans-violaxanthin. When those compounds are used as substrates, the major reaction product detected is a C14 dialdehyde: 4,9-dimethyldodeca-2,4,6,8,10-pentaene-1,12-dial. The enzyme did not cleave as efficiently carotenoids containing 9-cis-double or allenic bonds.
Computational Description
carotenoid cleavage dioxygenase 1 (CCD1); FUNCTIONS IN: 9-cis-epoxycarotenoid dioxygenase activity; INVOLVED IN: response to water deprivation, carotene catabolic process, xanthophyll catabolic process, carotenoid catabolic process; LOCATED IN: plasma membrane, vacuole, cytoplasm; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Carotenoid oxygenase (InterPro:IPR004294); BEST Arabidopsis thaliana protein match is: nine-cis-epoxycarotenoid dioxygenase 4 (TAIR:AT4G19170.1); Has 2926 Blast hits to 2896 proteins in 500 species: Archae - 16; Bacteria - 808; Metazoa - 317; Fungi - 194; Plants - 866; Viruses - 0; Other Eukaryotes - 725 (source: NCBI BLink).
Link
InterPro Scan - TAIR