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16-1720-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

This line has NOT been observed.

Determination of insertion point(s)

  • 5 prime edge [+] show sequence

    1. Chromosome: 4; Position: 1579765 [Map]; Strand: w
      • AT4G03560.1 - [+] show detail - two-pore channel 1
        Distance: -291; Region: promoter
    Flanking Genome Sequence: 16-1720-1 - 5-prime edge
    CACATTTCAC ATCTTTTCTT CTCCTTCTTA GTAGAGATCA GTTTCAGATT 
    AGATACTTGT CCATATTCCA CTACTCTCGT CTATTATCGG TTTCTGCTTG
    TCAATTTCTG GGTCCAAAAT TGAAATAAAT TTATATAATT GGACCACAAC
    ACCACTTTGT CGGTTTTGAC TTTTCTACTT GGATGATCCT GAACTTCTTG
    CTCCCTTTTC TATTACAAAT TGGGAAACAA TCAGTGTGAA TTGGGGAATA
    AAAAACAGAA TTGAGCTATT AAAGCCACGT GGCAAGAAAT CGACGAGGGA
    CCAACGTGTT TGTCTGCAAG ATCTAATCCT TTAATAACAA AACGGCGACT
    GATCTAACGG GAAAATTTTA AAATCGACAA AATTGTTAAT AATAAATTCA
    ACTACTTCTG GGCCATCATC ATCAATCTTG TTCGTCTTCC TTTTGATCTT
    ATCAAACTAC AGTCAAATCA TTCAGAGAAA GCTACTAGGT CTCTCCTCGA
    CATTTGCTTT CAATTCGATC CAGTAATTTT TTTTCTCATC AGAGAGAAAA
    AAAAAANGGA AAACCCNTNA ATNGGNAAAA ANANNCTTGG GGGGGGGGGN
    CGGANGGGTT NNNNNNNAAA ANCTTTNCCC CNTGGGNCNN CNNTNNAAAA
    NAANNNNNNC NNNNNNANNG GNNNTTTNTT NCCTNNNCC
  • 3 prime edge [+] show sequence

    1. Chromosome: 4; Position: 1579692 [Map]; Strand: w
      • AT4G03550.1 - [+] show detail - glucan synthase-like 5
        Distance: 0; Region: exon5 (3UTR)
      • AT4G03560.1 - [+] show detail - two-pore channel 1
        Distance: -364; Region: promoter
    Flanking Genome Sequence: 16-1720-1 - 3-prime edge
    ATNAAAACGG TAGAGGTATT TTACCGACCG TTACCGACCG TTTTCATCCT 
    GAACAAAAGC TCTGCGAGAT GACATTAGAT AACAATCTGA ATATAAACTA
    AAAATGCTCT AAAGCTCTAT AATGAGAGAA GGAGAACAAA TACACAAAGC
    CTCACTACAC AAATTGCTGT ACCTTTAGAA CAAAAACCAT ACAAGAATCA
    GAGTTCCCTC CACACACACT GACAATCAAA TATCTCCCTA AACCCATCTA
    ACCGAATCAA CCAACTTCCC GATTCCTCAT CCAACCAAAC TCTCCAAAAA
    CCTGTTTAAA ATACAAAGAT AAATCATCAG CATCTGAGTT TTTCTTTAGA
    CGAAACAAAA CTTCGAATT

AT4G03550.1

Model type
Protein coding
Short Description
glucan synthase-like 5
Curator Summary
Encodes a callose synthase that is required for wound and papillary callose formation in response to fungal pathogens Erysiphe and Blumeria. Mutants are resistant to P. parasitica and exhibit an exaggerated PR1 response.Contributes to PAMP-induced basal defense.
Computational Description
glucan synthase-like 5 (GSL05); FUNCTIONS IN: transferase activity, transferring glycosyl groups, 1,3-beta-glucan synthase activity; INVOLVED IN: in 12 processes; LOCATED IN: 1,3-beta-glucan synthase complex, plasma membrane; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Glycosyl transferase, family 48 (InterPro:IPR003440); BEST Arabidopsis thaliana protein match is: glucan synthase-like 1 (TAIR:AT4G04970.1); Has 1221 Blast hits to 894 proteins in 155 species: Archae - 2; Bacteria - 0; Metazoa - 0; Fungi - 695; Plants - 448; Viruses - 0; Other Eukaryotes - 76 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT4G03560.1

Model type
Protein coding
Short Description
two-pore channel 1
Curator Summary
Encodes a depolarization-activated Ca(2+) channel. Anti-sense experiments with this gene as well as Sucrose-H(+) symporters and complementation of yeast sucrose uptake mutant cch1 suggest that this protein mediates a voltage-activated Ca(2+ )influx. Mutants lack detectable SV channel activity suggesting TPC1 is essential component of the SV channel. Patch clamp analysis of loss of function mutation indicates TPC1 does not affect Ca2+ signaling in response to abiotic and biotic stress.
Computational Description
two-pore channel 1 (TPC1); FUNCTIONS IN: voltage-gated calcium channel activity, calcium channel activity; INVOLVED IN: regulation of jasmonic acid biosynthetic process, calcium ion transport, calcium-mediated signaling, seed germination, regulation of stomatal movement; LOCATED IN: vacuolar membrane, plasma membrane, vacuole, plant-type vacuole; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: EF-HAND 2 (InterPro:IPR018249), Ion transport (InterPro:IPR005821), EF-hand-like domain (InterPro:IPR011992), Calcium-binding EF-hand (InterPro:IPR002048); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
Link
InterPro Scan - TAIR