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16-2140-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

Specific phenotypes were NOT observed.

Determination of insertion point(s)

  • 5 prime edge [+] show sequence

    1. Chromosome: 1; Position: 1668609 [Map]; Strand: c
      • AT1G05580.2 - [+] show detail - cation/H+ exchanger 23
        Distance: 0; Region: exon1 (5UTR)
      • AT1G05580.1 - [+] show detail - cation/H+ exchanger 23
        Distance: 0; Region: exon1 (CDS)
      • AT1G05590.1 - [+] show detail - beta-hexosaminidase 2
        Distance: -741; Region: promoter
    Flanking Genome Sequence: 16-2140-1 - 5-prime edge 
    GTNNNNAGGA NNTTNANGNG NGGGAGNAAC CCGGGCNGGC ANAGAAGCAG 
    AGNACCGANA NAGACGGGAA GGGNANGTAA NAAAGAGNGG AANAATCNAG 
    GNNGNAACNN GNANGNGNCT NCTNGCGAAG NTCAAATCCT CNTACGNANG 
    CAATTNTNGN TACATCAATT NTNTAGCTNT CTAGTNCCGT ATGNGNCTNG 
    ATTTGTNGTG ATTGNATTTT TGCAGTGTGG ATTATTATTT AGCCCAAGTG 
    TGCTCGGGAA CACACGTNTC ATAATCGCAC ATGTATTCCC CTACAGATTC 
    ACAATGGTTC TNGAGACATT CGCTAACTTA GCTCTCGTCT ACAACATCTT 
    CCTCCTTGGT CTAGGGATGG ACCTGAGGAT GGNGCGGATC ACCGAGCTCA 
    AACCGGNCAT CATTGCTTTT ACAGGGCTTC TTGNTGCTTT ACCGGNTGGN 
    GCCTTTCTTT ACTATCTCCC GGGAAATGGC CATCCAGACA AAGANNATNT 
    CTGGNGGCGA NTNCNGGNCT GNCGGAGCTA GCCTGNACCA ACTNNCCTGA 
    CCNNGCAAGG ATC

  • 3 prime edge [+] show sequence

    1. Chromosome: 1; Position: 1668719 [Map]; Strand: c
      • AT1G05580.2 - [+] show detail - cation/H+ exchanger 23
        Distance: 0; Region: exon1 (5UTR)
      • AT1G05580.1 - [+] show detail - cation/H+ exchanger 23
        Distance: 0; Region: exon1 (CDS)
      • AT1G05590.1 - [+] show detail - beta-hexosaminidase 2
        Distance: -631; Region: promoter
    Flanking Genome Sequence: 16-2140-1 - 3-prime edge 
    NTTACNANCA NNNNGCNANT TNTNTTNAAA CAGANGANNG TGGTCGGGAC 
    ACANAGNCCT NGCCNTNCCC CGATTCATTN AATATCATAC GTTGGGATTN 
    GTCACACTTA AGGGGGGCTN CGGAAGACAT CTNGCTCCTA CGGATGGCTG 
    GGCGGAATCC CCAAAAAAAA TGACNATACT ATCTGCNTGA ACCCANCGTT 
    AGTTTCAGAC GAAACATTGG GTTCATATAG ACTGCAATAA CAACTAATTT 
    GCAACCTCTT CCAAGTTTTA GTTCANACGA GATGGCTAAT TATAGATGAT 
    GTATTAGTTG TCATTGGGGA ATT

AT1G05580.1

Model type
Protein coding
Short Description
cation/H+ exchanger 23
Curator Summary
member of Putative Na+/H+ antiporter family
Computational Description
cation/H+ exchanger 23 (CHX23); FUNCTIONS IN: monovalent cation:hydrogen antiporter activity, sodium:hydrogen antiporter activity; INVOLVED IN: cation transport; LOCATED IN: integral to membrane; EXPRESSED IN: male gametophyte, leaf; CONTAINS InterPro DOMAIN/s: Cation/H+ exchanger (InterPro:IPR006153); BEST Arabidopsis thaliana protein match is: cation/H+ exchanger 21 (TAIR:AT2G31910.1); Has 6204 Blast hits to 6178 proteins in 1654 species: Archae - 257; Bacteria - 4841; Metazoa - 0; Fungi - 132; Plants - 575; Viruses - 0; Other Eukaryotes - 399 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT1G05580.2

Model type
Protein coding
Short Description
cation/H+ exchanger 23
Curator Summary
member of Putative Na+/H+ antiporter family
Computational Description
cation/H+ exchanger 23 (CHX23); FUNCTIONS IN: monovalent cation:hydrogen antiporter activity, sodium:hydrogen antiporter activity; INVOLVED IN: cation transport; LOCATED IN: endomembrane system, integral to membrane; EXPRESSED IN: male gametophyte, leaf; CONTAINS InterPro DOMAIN/s: Cation/H+ exchanger (InterPro:IPR006153); BEST Arabidopsis thaliana protein match is: cation/H+ exchanger 21 (TAIR:AT2G31910.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT1G05590.1

Model type
Protein coding
Short Description
beta-hexosaminidase 2
Curator Summary
Encodes a protein with β-hexosaminidase activity (the enzyme is active with p-nitrophenyl-β-N-acetylglucosaminide as substrate but displayed only a minor activity toward p-nitrophenyl-β-N-acetylgalactosaminide). Chitotriose-PA was digested almost completely overnight by a 50% ammonium sulfate fraction of a supernatant yeast expressing AtHEX3.
Computational Description
beta-hexosaminidase 2 (HEXO2); FUNCTIONS IN: UDP-glucosyltransferase activity, hexosaminidase activity, beta-N-acetylhexosaminidase activity, hydrolase activity, hydrolyzing O-glycosyl compounds; INVOLVED IN: carbohydrate metabolic process; LOCATED IN: plasma membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Glycoside hydrolase, family 20, catalytic core (InterPro:IPR015883), Beta-N-acetylhexosaminidase, subunit a/b (InterPro:IPR015882), Glycoside hydrolase, catalytic core (InterPro:IPR017853), Glycoside hydrolase, family 20 (InterPro:IPR001540), Glycoside hydrolase, subgroup, catalytic core (InterPro:IPR013781); BEST Arabidopsis thaliana protein match is: beta-hexosaminidase 1 (TAIR:AT3G55260.1); Has 3806 Blast hits to 3725 proteins in 709 species: Archae - 2; Bacteria - 2403; Metazoa - 477; Fungi - 234; Plants - 128; Viruses - 0; Other Eukaryotes - 562 (source: NCBI BLink).
Link
InterPro Scan - TAIR