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Mutant Lines
53-2121-1

53-2121-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

This line has NOT been observed.

Determination of insertion point(s)

5 prime edge [+] show sequence

Chromosome: 1; Position: 24586180 [Map]; Strand: w
- AT1G66040.1 - [+] show detail - Zinc finger (C3HC4-type RING finger) family protein
  Distance: 0; Region: intron1 (CDS)

ATTAGGAACN ANAGNCACTA GATNTAACNN GTCTTTAACA CGGGTCCCAA 
GCATAGNTTA TCGATTTACT AGGGCTTATG TGTATTGATC ACTNCATCTA 
ACAAGTCANT TTTTTTTTTC TNANGCATNA ATTAACGAGT GAAAACCAAA 
CGTGACNGGT CTCTCTGGCN ATTGAATGCN NATNNAACAA AAGATCTCTA 
ACTTCTTCNT TNTCTCTCGA CNCA

3 prime edge [+] show sequence
1. Chromosome: 1; Position: 24586059 [Map]; Strand: w
  - AT1G66040.1 - [+] show detail - Zinc finger (C3HC4-type RING finger) family protein
    Distance: 0; Region: intron1 (CDS)
Flanking Genome Sequence: 53-2121-1 - 3-prime edge
```
AATANTANNA GAATCGCNAA TTTATGCGTG TGGTCTGTGT TAGAGCCTTG 
TNGGATCTTG TGATGAGTAC ACATAAGTGT CGTGAAATCG CTAGTTTANT 
TGTATACTTG ATANTGATGA TTAGTGTATT CAAGTAGCAG ACTAATGGAT 
GTATTGTTCA TCTCTCTCGA NGA
```

AT1G66040.1

Model type: Protein coding
Short Description: Zinc finger (C3HC4-type RING finger) family protein
Curator Summary: predicted to encode a protein with an N-terminal PHD domain and two RING domains surrounding an SRA domain. Attempts to isolate ORTH4/VIM4 cDNA through RT-PCR were unsuccessful and analysis of the expression of this gene is difficult since it shares 99% nucleotide identity with ORTH5/VIM2.
Computational Description: VARIANT IN METHYLATION 4 (VIM4); FUNCTIONS IN: zinc ion binding; LOCATED IN: cellular_component unknown; CONTAINS InterPro DOMAIN/s: Zinc finger, RING-type, conserved site (InterPro:IPR017907), Zinc finger, PHD-type, conserved site (InterPro:IPR019786), Zinc finger, RING-type (InterPro:IPR001841), Zinc finger, PHD-type (InterPro:IPR001965), SRA-YDG (InterPro:IPR003105), Zinc finger, C3HC4 RING-type (InterPro:IPR018957), Zinc finger, FYVE/PHD-type (InterPro:IPR011011); BEST Arabidopsis thaliana protein match is: Zinc finger (C3HC4-type RING finger) family protein (TAIR:AT1G66050.1); Has 6297 Blast hits to 5041 proteins in 427 species: Archae - 0; Bacteria - 14; Metazoa - 4562; Fungi - 413; Plants - 874; Viruses - 19; Other Eukaryotes - 415 (source: NCBI BLink).
Link: InterPro Scan - TAIR