1. Home
  2. Mutant Lines
  3. 53-2175-1

53-2175-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

This line has NOT been observed.

Determination of insertion point(s)

  • 5 prime edge [+] show sequence

    1. Chromosome: 3; Position: 18003261 [Map]; Strand: w
      • AT3G48560.1 - [+] show detail - chlorsulfuron/imidazolinone resistant 1
        Distance: 0; Region: exon1 (CDS)
    Flanking Genome Sequence: 53-2175-1 - 5-prime edge
    TAAGCGAATA CGGTTTCTAC GCCTTGACGT TCTAAAGCTT CGACGAGGAT 
    ATCAGCGCCT TTGCGGGGTT GATCTGGAGC GAATCGGGAG ATGAATGTTT
    CGGGTTTGGT AGGTTTGGTT GGAGAGGGAG TGGTTGTGAC ATTGGTGGTT
    GTGTTGAGCA CGGCGGAGAT GGAGGAGGGA GAGCTGGATT TGATACCGCG
    GCGGCGGGAG GAGGAGGATG ATTTGTTGGG GTTTAGGGAG AATGGGAGGG
    AGAATCTGGA GATTGGTAAT GGTGATTTGG AGGAGGAAGG AGATGGTTTG
    GTGGAGAAGG AGATCGAAGA AGATGTTGTT GTTGTTGTTG TTGCCGCCGC
    CATGGTTTAA TAAGAAGAGA AAAGAGTTCT TTTGTTATGG CTGAAGTAAT
    AGAGAAATGA GCTCGANCNA ANCNNGGNCT CNTACNACCC GCCCCNNNCC
    NTCN
  • 3 prime edge [+] show sequence

    1. Chromosome: 3; Position: 18003145 [Map]; Strand: w
      • AT3G48560.1 - [+] show detail - chlorsulfuron/imidazolinone resistant 1
        Distance: 0; Region: exon1 (CDS)
    Flanking Genome Sequence: 53-2175-1 - 3-prime edge
    TTCGCTTANC CTGGAGGTGC ATCAGTGGAG ATTCACCNAG CCTTAACCNG 
    CTCTTNCTCN NTCCGTAACG TCCTTCCTCG TCACGANNAA GGAGGTGTAT
    TCGCAGCANA AGGATACGCT CGATCCTCAG GTAAACCAGG TATCTGTATA
    GCCACTTCAT GTCCCNNAAC NACAAATCTC GTTAGCGGAT TAGCCGATGC
    GTTGTTAGAT AGTGTTCCTC TTGTAGCAAT CACAGGACAA GTCGCTCGTC
    GTATGATTGG TACAAGATGC GTTTCAAGAG ACTCCGATTG TTGAGGTAAC
    GCGTTCGATT ACNAAGCATA ACTATCTTGT GATGGATGT

AT3G48560.1

Model type
Protein coding
Short Description
chlorsulfuron/imidazolinone resistant 1
Curator Summary
Catalyzes the formation of acetolactate from pyruvate, the first step in valine and isoleucine biosynthesis. Requires FAD, thiamine pyrophosphate and Mg. Inhibited by the sulphonylurea herbicide, chlorsulphuron, and the imidazolinone herbicide, imazapyr. The obtained crystal structure of acetohydroxyacid synthase AHAS, EC 2.2.1.6)in complex with herbicides of the sulphonylurea and imidazolinone family reveals the molecular basis for substrate/inhibitor binding.
Computational Description
chlorsulfuron/imidazolinone resistant 1 (CSR1); FUNCTIONS IN: acetolactate synthase activity, pyruvate decarboxylase activity; INVOLVED IN: branched chain family amino acid biosynthetic process; LOCATED IN: chloroplast; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: TPP-binding enzyme, conserved site (InterPro:IPR000399), Thiamine pyrophosphate enzyme, central domain (InterPro:IPR012000), Acetolactate synthase, large subunit, biosynthetic (InterPro:IPR012846), Thiamine pyrophosphate enzyme, N-terminal TPP-binding domain (InterPro:IPR012001), Thiamine pyrophosphate enzyme, C-terminal TPP-binding (InterPro:IPR011766); BEST Arabidopsis thaliana protein match is: Thiamine pyrophosphate dependent pyruvate decarboxylase family protein (TAIR:AT5G17380.1); Has 30963 Blast hits to 30605 proteins in 2694 species: Archae - 488; Bacteria - 17421; Metazoa - 266; Fungi - 871; Plants - 599; Viruses - 25; Other Eukaryotes - 11293 (source: NCBI BLink).
Link
InterPro Scan - TAIR