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Mutant Lines
53-2645-1

53-2645-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

This line has NOT been observed.

Determination of insertion point(s)

5 prime edge [+] show sequence
1. Chromosome: 4; Position: 227941 [Map]; Strand: w
  - AT4G00500.1 - [+] show detail - alpha/beta-Hydrolases superfamily protein
    Distance: -545; Region: promoter
  - AT4G00500.2 - [+] show detail - alpha/beta-Hydrolases superfamily protein
    Distance: -746; Region: promoter
  - AT4G00520.2 - [+] show detail - Acyl-CoA thioesterase family protein
    Distance: 0; Region: exon2 (CDS)
  - AT4G00520.3 - [+] show detail - Acyl-CoA thioesterase family protein
    Distance: 0; Region: exon2 (CDS)
Flanking Genome Sequence: 53-2645-1 - 5-prime edge
```
CTTGGGAATG TGACCTTGTT GCAGCGATTA CCTAGTTCTT CTTTGAAGAG 
AATAGCTGAA GTTGTTCTCT TCAAACGTTA TGGTCAGTGA TTCTGGGATT 
TTGCTTTTGT TCNNNANNGG NNGNNNNNNG NGANNNNGNC NNNNNNNNNN 
NNNCTANAGA CNNNGGGNNA NTNTGNCGNN GANNNNCCNT ACNNGNNNNN 
NNNGNCNGCN NCGNTTACNN NNACNANNGA NNNATGNNNN GNATCNNGNG 
CGTGGGNNGN NNNGNNAGNC GCCNACNNAT NNNANANNGA CGAGCCCNNN 
CNGACTCNCN ANTNNCNCAN NCCGNNGTNA TGAGTNGAAN NGGTNNNNAN 
AATNN
```
3 prime edge [+] show sequence
1. Chromosome: 4; Position: 227825 [Map]; Strand: w
  - AT4G00500.1 - [+] show detail - alpha/beta-Hydrolases superfamily protein
    Distance: -429; Region: promoter
  - AT4G00500.2 - [+] show detail - alpha/beta-Hydrolases superfamily protein
    Distance: -630; Region: promoter
  - AT4G00520.2 - [+] show detail - Acyl-CoA thioesterase family protein
    Distance: 0; Region: intron1 (CDS)
  - AT4G00520.3 - [+] show detail - Acyl-CoA thioesterase family protein
    Distance: 0; Region: intron1 (CDS)
Flanking Genome Sequence: 53-2645-1 - 3-prime edge
```
TTCCCAAGGA ACTCCACAAC TACAAAACCA AAGCAACCAA TCATATGAAA 
AATCATCCGA ATCTTGGAAA AACTCTCAGT TCAACATCTC TTTCATCTC
```

AT4G00500.1

Model type: Protein coding
Short Description: alpha/beta-Hydrolases superfamily protein
Computational Description: alpha/beta-Hydrolases superfamily protein; FUNCTIONS IN: triglyceride lipase activity, calmodulin binding; INVOLVED IN: lipid catabolic process, lipid metabolic process; LOCATED IN: endomembrane system; EXPRESSED IN: 21 plant structures; EXPRESSED DURING: 9 growth stages; CONTAINS InterPro DOMAIN/s: Lipase, class 3 (InterPro:IPR002921), Mono-/di-acylglycerol lipase, N-terminal (InterPro:IPR005592); BEST Arabidopsis thaliana protein match is: alpha/beta-Hydrolases superfamily protein (TAIR:AT3G49050.1); Has 561 Blast hits to 561 proteins in 134 species: Archae - 0; Bacteria - 2; Metazoa - 171; Fungi - 76; Plants - 213; Viruses - 0; Other Eukaryotes - 99 (source: NCBI BLink).
Link: InterPro Scan - TAIR

AT4G00500.2

Model type: Protein coding
Short Description: alpha/beta-Hydrolases superfamily protein
Computational Description: alpha/beta-Hydrolases superfamily protein; FUNCTIONS IN: triglyceride lipase activity, calmodulin binding; INVOLVED IN: lipid catabolic process, lipid metabolic process; LOCATED IN: endomembrane system; EXPRESSED IN: 21 plant structures; EXPRESSED DURING: 9 growth stages; CONTAINS InterPro DOMAIN/s: Lipase, class 3 (InterPro:IPR002921), Mono-/di-acylglycerol lipase, N-terminal (InterPro:IPR005592); BEST Arabidopsis thaliana protein match is: alpha/beta-Hydrolases superfamily protein (TAIR:AT3G49050.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).
Link: InterPro Scan - TAIR

AT4G00520.2

Model type: Protein coding
Short Description: Acyl-CoA thioesterase family protein
Computational Description: Acyl-CoA thioesterase family protein; FUNCTIONS IN: acyl-CoA thioesterase activity; INVOLVED IN: acyl-CoA metabolic process; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Cyclic nucleotide-binding (InterPro:IPR000595), Cyclic nucleotide-binding-like (InterPro:IPR018490), Acyl-CoA thioesterase (InterPro:IPR003703), RmlC-like jelly roll fold (InterPro:IPR014710); BEST Arabidopsis thaliana protein match is: Acyl-CoA thioesterase family protein (TAIR:AT1G01710.1); Has 3429 Blast hits to 3402 proteins in 976 species: Archae - 0; Bacteria - 1910; Metazoa - 393; Fungi - 300; Plants - 65; Viruses - 0; Other Eukaryotes - 761 (source: NCBI BLink).
Link: InterPro Scan - TAIR

AT4G00520.3

Model type: Protein coding
Short Description: Acyl-CoA thioesterase family protein
Computational Description: Acyl-CoA thioesterase family protein; FUNCTIONS IN: acyl-CoA thioesterase activity; INVOLVED IN: acyl-CoA metabolic process; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Cyclic nucleotide-binding (InterPro:IPR000595), Cyclic nucleotide-binding-like (InterPro:IPR018490), Acyl-CoA thioesterase (InterPro:IPR003703), RmlC-like jelly roll fold (InterPro:IPR014710); BEST Arabidopsis thaliana protein match is: Acyl-CoA thioesterase family protein (TAIR:AT1G01710.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
Link: InterPro Scan - TAIR