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Mutant Lines
53-3100-1

53-3100-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

This line has NOT been observed.

Determination of insertion point(s)

5 prime edge [+] show sequence

Chromosome: 5; Position: 7619906 [Map]; Strand: c
- AT5G22800.1 - [+] show detail - Alanyl-tRNA synthetase, class IIc
  Distance: 0; Region: exon1 (CDS)

CTCCTCATAC TCTCTGATGA ATTTCTCCAG AGTAAACCTC TTCGATTTTC 
CTCTTAGACC AATTTTACTT TCGCATCCTT CTTCTATTTT CGTTTCTACA 
CGTAAGTGTT NTCTCCCTTT TAGCAGAATC TATCTCAACC TGTAAAATTA 
GCTCTAGCCT TTGTCTAATT GATGAAACTG ATATCTGGGT TTTGTCTTCC 
GTCGCAGTAG AGAACTTTCC TAATTGTTTT TTTTTTT

3 prime edge [+] show sequence
1. Chromosome: 5; Position: 7620022 [Map]; Strand: c
  - AT5G22800.1 - [+] show detail - Alanyl-tRNA synthetase, class IIc
    Distance: -41; Region: promoter
Flanking Genome Sequence: 53-3100-1 - 3-prime edge
```
ATGAGGAGGG AGAGTTTCAG GGAGAAGAAG AAGACACACT AGCTAGCCTT 
CAAGCGTTTT TTTATCCACT TCGTTTGGTT TGAAAGATTT TTAATCAAAA 
AAAAATTA
```

AT5G22800.1

Model type: Protein coding
Short Description: Alanyl-tRNA synthetase, class IIc
Curator Summary: A locus involved in embryogenesis. Mutations in this locus result in embryo lethality.
Computational Description: EMBRYO DEFECTIVE 1030 (EMB1030); FUNCTIONS IN: alanine-tRNA ligase activity, ligase activity, forming aminoacyl-tRNA and related compounds, nucleotide binding, ATP binding, nucleic acid binding; INVOLVED IN: alanyl-tRNA aminoacylation, embryo development ending in seed dormancy; LOCATED IN: mitochondrion, chloroplast stroma, chloroplast, membrane; EXPRESSED IN: cultured cell, leaf; CONTAINS InterPro DOMAIN/s: Threonyl/alanyl tRNA synthetase, class II-like, putative editing domain (InterPro:IPR018163), Alanyl-tRNA synthetase, class IIc (InterPro:IPR002318), Alanyl-tRNA synthetase, class IIc, anti-codon-binding domain (InterPro:IPR018162), Alanyl-tRNA synthetase, class IIc, core domain (InterPro:IPR018165), Threonyl/alanyl tRNA synthetase, SAD (InterPro:IPR012947), Alanyl-tRNA synthetase, class IIc, N-terminal (InterPro:IPR018164), Phosphoesterase, DHHA1 (InterPro:IPR003156); BEST Arabidopsis thaliana protein match is: Alanyl-tRNA synthetase (TAIR:AT1G50200.1); Has 15847 Blast hits to 15731 proteins in 3137 species: Archae - 486; Bacteria - 7745; Metazoa - 750; Fungi - 242; Plants - 138; Viruses - 0; Other Eukaryotes - 6486 (source: NCBI BLink).
Link: InterPro Scan - TAIR