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53-3258-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

This line has NOT been observed.

Determination of insertion point(s)

  • 5 prime edge [+] show sequence

    1. Chromosome: 5; Position: 24205710 [Map]; Strand: w
      • AT5G60110.1 - [+] show detail - pumilio 18
        Distance: 2773; Region: intergenic
      • AT5G60120.2 - [+] show detail - target of early activation tagged (EAT) 2
        Distance: -1979; Region: intergenic
    Flanking Genome Sequence: 53-3258-1 - 5-prime edge 
    GTGANAAACT AACAAAATCG GTTATACGAT AACGGTCGGT ACGGGATTTT 
    CCCATCCTAC TTTCATCCAN GNCATNNTCA TNCNNNNGNC CGTGACCTGT 
    GNTANGAACN NGGTACNNCG NNACGNACCT GTNTATNACT NTTNGNTAAT 
    AAACAATAAT TTCCTTTGAT TGTAAAAGAA GCCCCCTTAT TGGCTTTTGT 
    TCTTTCCTAT GTCTTCTTGT ACTCAAAATA TATTTTGTTT TAGACTTTCG 
    AAATTATCCA TTTTGGAAAC CTATCCATTT CTTTGTTGAT CTCTTTGTAT 
    CACATTGCTC TCAAGCTATG TAGTAGTCTT AGAGATTTAG ACAATACTAA 
    TGAATACAAG ATACCATAAG ATTTGATACA ATATGTTTGT GTTATCAACG 
    TTTGATCGGA TATGTGTTTT TTTTTCTACT TATAGCTATT CATTCAATGA 
    AAATCTAAAA GAAAATTGAG ACACAGTTGA TGCAGAGATA ATTTTCTCTA 
    NACTTGATAG ATTTTTACAA TGTGATATTG TAGGAACTAG GAAACACATG 
    CTTCATTTTT ATTTACTAAA ACTTGGCTAC AAACAAGGCG AAGATAACAT 
    ACATCATTCA ATTTCAATAG TAATTAATGN GTCAAAACCT TAGAAATTAA 
    AACAAAATTT CATCACTATG TCCAGTAAGA AAAACTAAAG CTATCAAACC 
    TGATGTTAGN GATTTTTTTC CCCATCATCT TTAACTATTT TTCCATACAC 
    ATTCGGTTTT TTGTAATAAT AACAACTTNT TAAAATGATA T

  • 3 prime edge [+] show sequence

    1. Chromosome: 5; Position: 24205659 [Map]; Strand: w
      • AT5G60110.1 - [+] show detail - pumilio 18
        Distance: 2722; Region: intergenic
      • AT5G60120.2 - [+] show detail - target of early activation tagged (EAT) 2
        Distance: -2030; Region: intergenic
    Flanking Genome Sequence: 53-3258-1 - 3-prime edge 
    CCTAAAAATA TCTATTCTTT GTGTCCATAC GAATCTCTTT TTTCTTTGGA 
    AATGTTTATT GAGTGGGGTA GGTCAAATGA ACATTCCGAC TGGTTTTAGC 
    CGGTTGCCAA AACTTAAGAG TGTCAGAAAA ATCTCGAAGA TCATGGATTT 
    TAACGAGTAG TATAAAGCCA CGTGTACAAC AGAGATTGAA CCCCAGCCAT 
    TCAACTACTC TCTCAGCCAT CTTAGGTCAC ATATCTGATC GGACGGCCCA 
    TGTCCATTCT TACTTTCCTT AAATTTAGGG AATTTAACAG TTTCACAATT 
    TATCTAGTTT TTTTTTTT

AT5G60110.1

Model type
Protein coding
Short Description
pumilio 18
Curator Summary
Encodes a member of the Arabidopsis Pumilio (APUM) proteins containing PUF domain (eight repeats of approximately 36 amino acids each). PUF proteins regulate both mRNA stability and translation through sequence-specific binding to the 3' UTR of target mRNA transcripts.
Computational Description
pumilio 18 (PUM18); FUNCTIONS IN: RNA binding, binding; LOCATED IN: cytoplasm; CONTAINS InterPro DOMAIN/s: Pumilio RNA-binding repeat (InterPro:IPR001313), Armadillo-like helical (InterPro:IPR011989), Armadillo-type fold (InterPro:IPR016024); BEST Arabidopsis thaliana protein match is: pumilio 19 (TAIR:AT5G60180.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT5G60120.2

Model type
Protein coding
Short Description
target of early activation tagged (EAT) 2
Computational Description
target of early activation tagged (EAT) 2 (TOE2); FUNCTIONS IN: DNA binding, sequence-specific DNA binding transcription factor activity; INVOLVED IN: regulation of transcription, DNA-dependent; LOCATED IN: nucleus; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: DNA-binding, integrase-type (InterPro:IPR016177), Pathogenesis-related transcriptional factor/ERF, DNA-binding (InterPro:IPR001471); BEST Arabidopsis thaliana protein match is: related to AP2.7 (TAIR:AT2G28550.2).
Link
InterPro Scan - TAIR