1. Home
  2. Mutant Lines
  3. 53-3281-1

53-3281-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

This line has NOT been observed.

Determination of insertion point(s)

  • 5 prime edge [+] show sequence

    1. Chromosome: 3; Position: 1992656 [Map]; Strand: c
      • AT3G06483.1 - [+] show detail - pyruvate dehydrogenase kinase
        Distance: 0; Region: exon1 (5UTR)
    Flanking Genome Sequence: 53-3281-1 - 5-prime edge 
    GATAAACTAA CAAAATCGGT TATACGATAA CGGTCGGTAC GGGATTTTCC 
    CATCCTACTT TCATCCCAGT CGATGATAAT AACGGTGGAG AACGACGGAG 
    GCGGGCGACG TTAGGGTTTC TAATCATTTC TCTCTCTTAG ACGCTTATGG 
    CAGTGAAGAA AGCCTGCGAA ATGTTCCCGA AGAGTTTGAT CGAAGATGTT 
    CACAAATGGG GTTGCATGAA GCAAACCGGT GTTAGCCTTA GATACATGAT 
    GGAGTTTGGT TCCAAACCTA CTGAGAGGAA TCTTTTGATT TCTGCTCAGT 
    TTTTGCATAA GGAGCTTCCG ATTCGCGTCG CCAGGAGAGC GATCGAACTC 
    CAGACGCTTC CTTATGGTCT CTCTGATAAA CCTGCCGTTT TGAAGGTACT 
    CTTTCTGGGA GTTTCGAATT TTGTTGTTTC TAGAATT

  • 3 prime edge [+] show sequence

    1. Chromosome: 3; Position: 1992710 [Map]; Strand: c
      • AT3G06483.1 - [+] show detail - pyruvate dehydrogenase kinase
        Distance: 0; Region: exon1 (5UTR)
    Flanking Genome Sequence: 53-3281-1 - 3-prime edge 
    GCTGGACGAA AGAAGTGCGA AGATGATTGG TGGACATCCA GAAACAGAGA 
    AGAAGAGGAT TGGCTTTGGC TTATGGCAAT CAACATGTGC TGCTAATATC 
    GTTGTTTTTT TTGTTTTGGC CGCTAATTTT CTTGGATTAG GATTTTTACT 
    CTTTCTGGAT GAATATTTTT TTAGTATTTA CCTGATTTGG TAAAAAGTTT 
    CTTTTTGTTG TGTTAAAGAA TAACCGTATA TTTATTTTAT TTTATGTAAG 
    AAAAAATAAT AATATGTAAA AATCAAAATA ATGTTAAACT GTTCAAATAA 
    ACATCTCACT AGTTTGCTTC TTTTTTTTCC ACAATCTCAT TAATTTGCTT 
    AGAATT

AT3G06483.1

Model type
Protein coding
Short Description
pyruvate dehydrogenase kinase
Curator Summary
Pyruvate dehydrogenase kinase (PDK) specifically phosphorylates the E1α subunit of the pyruvate dehydrogenase complex (PDC) on a Ser residue using ATP as a phosphate donor. PDK is a unique type of protein kinase having a His-kinase-like sequence but Ser-kinase activity. Site-directed mutagenesis and structural analysis indicate that PDK belongs to the GHKL superfamily.
Computational Description
pyruvate dehydrogenase kinase (PDK); FUNCTIONS IN: ATP binding, pyruvate dehydrogenase (acetyl-transferring) kinase activity, histidine phosphotransfer kinase activity; INVOLVED IN: signal transduction, peptidyl-histidine phosphorylation, phosphorylation; LOCATED IN: mitochondrion; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Signal transduction histidine kinase, core (InterPro:IPR005467), Branched-chain alpha-ketoacid dehydrogenase kinase/Pyruvate dehydrogenase kinase, mitochondrial (InterPro:IPR018955), ATPase-like, ATP-binding domain (InterPro:IPR003594), Signal transduction histidine kinase-related protein, C-terminal (InterPro:IPR004358); Has 1925 Blast hits to 1799 proteins in 531 species: Archae - 0; Bacteria - 712; Metazoa - 467; Fungi - 468; Plants - 102; Viruses - 0; Other Eukaryotes - 176 (source: NCBI BLink).
Link
InterPro Scan - TAIR