1. Home
  2. Mutant Lines
  3. 53-4015-1

53-4015-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

This line has NOT been observed.

Determination of insertion point(s)

  • 5 prime edge [+] show sequence

    1. Chromosome: 5; Position: 24204022 [Map]; Strand: c
      • AT5G60110.1 - [+] show detail - pumilio 18
        Distance: 1085; Region: intergenic
      • AT5G60120.2 - [+] show detail - target of early activation tagged (EAT) 2
        Distance: -3667; Region: intergenic
    Flanking Genome Sequence: 53-4015-1 - 5-prime edge 
    GGGATAAACT AACAAAATCG GTTATACGAT AACGGTCGGT ACGGGATTTT 
    CCCATCCTAC TTTCATCCCC GCCGTTGACT CCTCCGCATA CAGAAGTTAC 
    TACATACTTC TAAAACGAGA GCTCAATGCA ATGACCATAA AGATTAACTG 
    CGATGATCCC TAGATAAAAT GGATTTGATC TTGTCACTTG CTAATGAGAG 
    CATTAATGTC AAATTATAAA GATTTTAACT AATGCGAGTT TTAATTTTTT 
    TTACATTAAT TGACTTGTAT TCATAGAACA TTTGTAGCAA AAACTTGGAA 
    AACAGAGGTT TCTCTCTATT GTTATACTTG TAGGGCTGCA AATTGGAAAA 
    AGAAAAAACA CTGCTAAAAC GCCGTCGTTT TCGAAAATTA GTCATTAGAG 
    GCCCACGGGA ACACGATATA TGGGCCTAAG TTTCGGTCAA TTCTATATAA 
    GAAAATGACA ATACTGTCCT AAATAATAGA GGCACAGAGA CAGAGAAAAG 
    CT

  • 3 prime edge [+] show sequence

    1. Chromosome: 5; Position: 24204025 [Map]; Strand: c
      • AT5G60110.1 - [+] show detail - pumilio 18
        Distance: 1088; Region: intergenic
      • AT5G60120.2 - [+] show detail - target of early activation tagged (EAT) 2
        Distance: -3664; Region: intergenic
    Flanking Genome Sequence: 53-4015-1 - 3-prime edge 
    ATGAAAACGG TAGAGGTATT TTACCGACCG TTACCGACCG TTTTCAACGG 
    TAGTGGTTGT TGTGGAGCTT TTGGAGTGCG ACGGAGATAG GTTGATGAGG 
    CTGGCAAAGA TTGAGTTTGG GAATATCGTG GTAGGCAAGG CACTGAGGTT 
    CTCGAAGGAG ATATCTAGGA CTAGGATTGA TTTTTTTTGG GGTTTGATGC 
    AGAAGCTCAA GCATTTTTCT CCATCTCTTG GTGTAGGTCT CAGGGAAACA 
    ACATTGCAAA CATCTTGGAC TCATTTAAGC T

AT5G60110.1

Model type
Protein coding
Short Description
pumilio 18
Curator Summary
Encodes a member of the Arabidopsis Pumilio (APUM) proteins containing PUF domain (eight repeats of approximately 36 amino acids each). PUF proteins regulate both mRNA stability and translation through sequence-specific binding to the 3' UTR of target mRNA transcripts.
Computational Description
pumilio 18 (PUM18); FUNCTIONS IN: RNA binding, binding; LOCATED IN: cytoplasm; CONTAINS InterPro DOMAIN/s: Pumilio RNA-binding repeat (InterPro:IPR001313), Armadillo-like helical (InterPro:IPR011989), Armadillo-type fold (InterPro:IPR016024); BEST Arabidopsis thaliana protein match is: pumilio 19 (TAIR:AT5G60180.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT5G60120.2

Model type
Protein coding
Short Description
target of early activation tagged (EAT) 2
Computational Description
target of early activation tagged (EAT) 2 (TOE2); FUNCTIONS IN: DNA binding, sequence-specific DNA binding transcription factor activity; INVOLVED IN: regulation of transcription, DNA-dependent; LOCATED IN: nucleus; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: DNA-binding, integrase-type (InterPro:IPR016177), Pathogenesis-related transcriptional factor/ERF, DNA-binding (InterPro:IPR001471); BEST Arabidopsis thaliana protein match is: related to AP2.7 (TAIR:AT2G28550.2).
Link
InterPro Scan - TAIR