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53-4218-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

This line has NOT been observed.

Determination of insertion point(s)

  • 5 prime edge [+] show sequence

    1. Chromosome: 3; Position: 23458876 [Map]; Strand: w
      • AT3G63530.1 - [+] show detail - RING/U-box superfamily protein
        Distance: -417; Region: promoter
      • AT3G63530.2 - [+] show detail - RING/U-box superfamily protein
        Distance: -441; Region: promoter
    Flanking Genome Sequence: 53-4218-1 - 5-prime edge
    AGTCTACTGC AGACTAGCTT TGTTTTTTTT TCTTTTCTAA ATCTTATATT 
    CATACATTTT GNGTCTCTAC GAATTTTTAG TTTAGGGTTT AATTTGTAGA
    GCATAGCCTG TGCTAGTATA AAAAGTGTGC NTTAGATGGA TC
  • 3 prime edge [+] show sequence

    1. Chromosome: 3; Position: 23458765 [Map]; Strand: w
      • AT3G63530.1 - [+] show detail - RING/U-box superfamily protein
        Distance: -306; Region: promoter
      • AT3G63530.2 - [+] show detail - RING/U-box superfamily protein
        Distance: -330; Region: promoter
    Flanking Genome Sequence: 53-4218-1 - 3-prime edge
    GTGCCAGTAG TAATAAAAAG AAGAAGCATG GAAGGGAGGT TTCCTTTTGT 
    GGGAAAACAG ACAAGCAAGT TGTGGTTTTG ATAATCTCAA GGCCGAAAGC
    TGTACTTTTG ATTTAAATAT GTGATCCATC AATGAAGAAC AAATACTCTC
    TCATTCCTTT TTTTTAATTT AAAGGAAGAA AGAGAAAAAT TAAAGAAGCA
    AATCTTTGTC TCTGGGTCTG AGCACGGGAC TTGAAGACAG ATGTAGGGTT
    GAGATGACAT GTAAGGGTGG GTGCGTCTCC CCACACTGAA CCACATATCT
    TTTTGCCGGC TTCTTCAACG GCTTTTTCTC TTCCCCCTTC CCTTCCCTTC
    CCTTCCTTCG ACGTCGTTTT GTCTCCTTCC ACACACTCTT TCCTCTCTCT
    TTCTTCTCTC TTTCTTTTCT CTCTCTCTCC TCTGCTCCTC CGTCTCTCGT
    CTACAGTGCC CTCCGCATCA CCTTTTTCCT TGTCCTATGA ATTTGGTCGA
    AATGCCCTTC TCCTCCTCCT CCTTCCACTA ATCTCAAGTA AGTCTCTTTC
    TTCTCTCACT GTTTTCAATT TCAGNCCTTT TTTCTTTCTT TCTTCTTGAT
    TTGTTTTGCG GGGCANAAGA TATATCCTTC GAGACTCTCC CTTGCCGTCT
    NCAATTGCCA CTCACCGCTC CAACTCTCTT CGAATGTATG TATGCCTCTN
    CTTNCCTGCG CTTTCAATTC TTTGTATGCC TAAGNCTG

AT3G63530.1

Model type
Protein coding
Short Description
RING/U-box superfamily protein
Curator Summary
Encodes a novel E3 ubiquitin ligase that acts as a central negative regulator of Arabidopsis floral organ size. Organ size is limited by restricting the period of proliferative growth, rather than the rate of growth and appears to act in a novel pathway, independent of ANT and JAG. Both mRNA and protein are expressed in all actively growing regions of the plant and the vasculature and the protein is rapidly turned over by proteasome-mediated degradation.
Computational Description
BIG BROTHER (BB); CONTAINS InterPro DOMAIN/s: Zinc finger, RING-type (InterPro:IPR001841), Zinc finger, C3HC4 RING-type (InterPro:IPR018957); BEST Arabidopsis thaliana protein match is: RING/U-box superfamily protein (TAIR:AT3G19910.1); Has 8059 Blast hits to 8045 proteins in 262 species: Archae - 0; Bacteria - 0; Metazoa - 2322; Fungi - 418; Plants - 4266; Viruses - 8; Other Eukaryotes - 1045 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT3G63530.2

Model type
Protein coding
Short Description
RING/U-box superfamily protein
Curator Summary
Encodes a novel E3 ubiquitin ligase that acts as a central negative regulator of Arabidopsis floral organ size. Organ size is limited by restricting the period of proliferative growth, rather than the rate of growth and appears to act in a novel pathway, independent of ANT and JAG. Both mRNA and protein are expressed in all actively growing regions of the plant and the vasculature and the protein is rapidly turned over by proteasome-mediated degradation.
Computational Description
BIG BROTHER (BB); CONTAINS InterPro DOMAIN/s: Zinc finger, RING-type (InterPro:IPR001841), Zinc finger, C3HC4 RING-type (InterPro:IPR018957); BEST Arabidopsis thaliana protein match is: RING/U-box superfamily protein (TAIR:AT3G19910.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).
Link
InterPro Scan - TAIR