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Mutant Lines
53-4455-1

53-4455-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

This line has NOT been observed.

Determination of insertion point(s)

Notice

More than one insertion position or no insertion point is expected.

5 prime edge [+] show sequence

Chromosome: 4; Position: 245932 [Map]; Strand: w
- AT4G00570.1 - [+] show detail - NAD-dependent malic enzyme 2
  Distance: 0; Region: intron4 (CDS)

GGGATAAACT AACAAAATCG GTTATACGAT AACGGTCGGT ACGGGATTTT 
CCCATCCTAC TTTCATCCCC AAATGTGGGA CAGTATTTAG AGCCAAAAAG 
ACTCACTCGG TAGTACAAAG TCTCATTACG GTCATGCAAT CTATTCAACA 
TCCTCCATTT AGCTAATGCA ACAACGTTTT CAGGCTCTCC TTTTGTGTTG 
TTCTCTAGCG AACGAAACGA CTCAACTGCC AAATTATGTT TCAAAACCAA 
AAGTCTCAGA AACCCGAAAA GAAACATACA AAGATTTTAA CTTTGGACAA 
TGGATCAAAA CTTACTGAAA CGATCACATT GCTGTACACA GGTCATAACA 
CGAGGAGGAA GCAAGCCTCG TATCCCTAAT CTATCTCTCT CAGTCAAAGG 
AAAACCTGTG TCCTGCAAAA GCACCAAAGA GATAGAGAGA CCATAACAAC 
AACAACCATC AGCATTATAA TCAATTTTTA GAACCCTAAA ATTCAGAAGA 
TCGAGGATAA AACACACAAC CTTATTGAAC CATGGGATCG TGAAGAATAT 
CAGCACCACG CTTGTGGACG ATGCAAGGAC CAGGAATCGC TGTGGAAAAG 
CACCGCCGAG ATCCGTGTGT TCTTGCCGCT GCCGCTGCCT TCGACAACCC 
AGCAATGTTC TTCCACATCA TCGGTCTCGA AATATCACTC AGGATC

3 prime edge [+] show sequence

Chromosome: 4; Position: 227894 [Map]; Strand: c
- AT4G00500.1 - [+] show detail - alpha/beta-Hydrolases superfamily protein
  Distance: -498; Region: promoter
- AT4G00500.2 - [+] show detail - alpha/beta-Hydrolases superfamily protein
  Distance: -699; Region: promoter
- AT4G00520.2 - [+] show detail - Acyl-CoA thioesterase family protein
  Distance: 0; Region: exon2 (CDS)
- AT4G00520.3 - [+] show detail - Acyl-CoA thioesterase family protein
  Distance: 0; Region: exon2 (CDS)

ATGAAAACGG TAGAGGTATT TTACCGACCG TTACCGACCG TTTTCATTGC 
AGCGATTACC TAGTTCTTCT TTGAAGAGAA TAGCTGAAGT TGTTCTCTTC 
AAACGTTATG GTCAGTGATT CTGGGATTTT GCTTTTGTTC TTATCTTGAA 
GTCAGTTTGA TTGGGAATTT CGTTATGTTT TGTTGATCTG TATTCAGTGT 
CAGTGCAGTG TTCTTGAGTA TAAGCACTTG AGCAGTGATT TTAGAGACAG 
ATATTTATGT TTTTACACAA TGTGATCTTC TTTGGCAATG TGCTTCACAG 
ACAGAGGTGA TTATGTGGTT CGTGAAAATC AAAATGTGGA TGGAGTTTAT 
TTTCTCTTGC AAGGACAGGT TTGTCTTCTC AGGTTACTTC TGTGTTATAT 
AGTCTTATAG ATAGACTAAG GCTACTGCGC CGTGTTGATT TGTAACTATT 
TTTGACTTGT GTTTGCAGGC TCAGGTTCTG AGATCAGCTA AAGATGAAAA 
CTGTCAAGAG TTACCTTTGA AACGATATGA TTTCTTCGGC CATGGTGAGA 
GGAAGAATCT TTCAATACTG GCCTTTCTTC TGTTTCAGTG TGTTAATTTT 
TGTTTATGGT TATTGAATTG GCTTCAATTA GTTCTCAAAC AGGTATTTTT 
GGGGGATGTT TACTCAGCAG ATGTTGTTGC ATTGTCAGAN GTGTTTGCTA 
AAAATTCTCT CTTCTTTCCT TANGTGGCCT TGGTTTCCTT GAGCTCATGC 
TATGCTAATG GTAATTGCAT ACT

AT4G00500.1

Model type: Protein coding
Short Description: alpha/beta-Hydrolases superfamily protein
Computational Description: alpha/beta-Hydrolases superfamily protein; FUNCTIONS IN: triglyceride lipase activity, calmodulin binding; INVOLVED IN: lipid catabolic process, lipid metabolic process; LOCATED IN: endomembrane system; EXPRESSED IN: 21 plant structures; EXPRESSED DURING: 9 growth stages; CONTAINS InterPro DOMAIN/s: Lipase, class 3 (InterPro:IPR002921), Mono-/di-acylglycerol lipase, N-terminal (InterPro:IPR005592); BEST Arabidopsis thaliana protein match is: alpha/beta-Hydrolases superfamily protein (TAIR:AT3G49050.1); Has 561 Blast hits to 561 proteins in 134 species: Archae - 0; Bacteria - 2; Metazoa - 171; Fungi - 76; Plants - 213; Viruses - 0; Other Eukaryotes - 99 (source: NCBI BLink).
Link: InterPro Scan - TAIR

AT4G00500.2

Model type: Protein coding
Short Description: alpha/beta-Hydrolases superfamily protein
Computational Description: alpha/beta-Hydrolases superfamily protein; FUNCTIONS IN: triglyceride lipase activity, calmodulin binding; INVOLVED IN: lipid catabolic process, lipid metabolic process; LOCATED IN: endomembrane system; EXPRESSED IN: 21 plant structures; EXPRESSED DURING: 9 growth stages; CONTAINS InterPro DOMAIN/s: Lipase, class 3 (InterPro:IPR002921), Mono-/di-acylglycerol lipase, N-terminal (InterPro:IPR005592); BEST Arabidopsis thaliana protein match is: alpha/beta-Hydrolases superfamily protein (TAIR:AT3G49050.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).
Link: InterPro Scan - TAIR

AT4G00520.2

Model type: Protein coding
Short Description: Acyl-CoA thioesterase family protein
Computational Description: Acyl-CoA thioesterase family protein; FUNCTIONS IN: acyl-CoA thioesterase activity; INVOLVED IN: acyl-CoA metabolic process; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Cyclic nucleotide-binding (InterPro:IPR000595), Cyclic nucleotide-binding-like (InterPro:IPR018490), Acyl-CoA thioesterase (InterPro:IPR003703), RmlC-like jelly roll fold (InterPro:IPR014710); BEST Arabidopsis thaliana protein match is: Acyl-CoA thioesterase family protein (TAIR:AT1G01710.1); Has 3429 Blast hits to 3402 proteins in 976 species: Archae - 0; Bacteria - 1910; Metazoa - 393; Fungi - 300; Plants - 65; Viruses - 0; Other Eukaryotes - 761 (source: NCBI BLink).
Link: InterPro Scan - TAIR

AT4G00520.3

Model type: Protein coding
Short Description: Acyl-CoA thioesterase family protein
Computational Description: Acyl-CoA thioesterase family protein; FUNCTIONS IN: acyl-CoA thioesterase activity; INVOLVED IN: acyl-CoA metabolic process; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Cyclic nucleotide-binding (InterPro:IPR000595), Cyclic nucleotide-binding-like (InterPro:IPR018490), Acyl-CoA thioesterase (InterPro:IPR003703), RmlC-like jelly roll fold (InterPro:IPR014710); BEST Arabidopsis thaliana protein match is: Acyl-CoA thioesterase family protein (TAIR:AT1G01710.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
Link: InterPro Scan - TAIR

AT4G00570.1

Model type: Protein coding
Short Description: NAD-dependent malic enzyme 2
Curator Summary: Encodes an NAD-dependent malic enzyme (NAD-ME) that does not act on oxaloacetate, indicating that it belongs to EC 1.1.1.39. It is a member of the beta family of NAD-MEs in plants. It appears to function as a homodimer or as a heterodimer with the alpha-type NAD-ME2 (At2g13560). NAD-ME2 transcript and protein levels are higher during the night than during the day.
Computational Description: NAD-dependent malic enzyme 2 (NAD-ME2); FUNCTIONS IN: in 7 functions; INVOLVED IN: malate metabolic process; LOCATED IN: mitochondrion, chloroplast; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Malic enzyme, NAD-binding (InterPro:IPR012302), Malic oxidoreductase (InterPro:IPR001891), Malic enzyme, conserved site (InterPro:IPR015884), Malic enzyme, N-terminal (InterPro:IPR012301), NAD(P)-binding domain (InterPro:IPR016040); BEST Arabidopsis thaliana protein match is: NAD-dependent malic enzyme 1 (TAIR:AT2G13560.1); Has 9343 Blast hits to 9326 proteins in 2439 species: Archae - 143; Bacteria - 6323; Metazoa - 607; Fungi - 223; Plants - 463; Viruses - 0; Other Eukaryotes - 1584 (source: NCBI BLink).
Link: InterPro Scan - TAIR