1. Home
  2. Mutant Lines
  3. 53-4456-1

53-4456-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

This line has NOT been observed.

Determination of insertion point(s)

  • 5 prime edge [+] show sequence

    1. Chromosome: 5; Position: 25645321 [Map]; Strand: c
      • AT5G64070.1 - [+] show detail - phosphatidylinositol 4-OH kinase beta1
        Distance: -875; Region: promoter
      • AT5G64080.1 - [+] show detail - Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfa...
        Distance: 0; Region: exon3 (3UTR)
      • AT5G64080.2 - [+] show detail - Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfa...
        Distance: 0; Region: exon3 (3UTR)
    Flanking Genome Sequence: 53-4456-1 - 5-prime edge 
    TGTTTTGGAG TGTTCTGTTT TTAGACCATA TCGGTTTCTC TATTACTAGC 
    GTTTACTCTT TTAGTTTATG TTATTACTTT GTCTCACAAC ATTGGTCCTT 
    TTATTCGATC TATCGCTTAT TCCTTCATCT ATGTTTTCTC TCACACCCTC 
    ATAGTTATAT TCGCCAAAAG CTCAGGCAGA AACATTCTCA AAGTCCACTA 
    TACAAAAAAG AAATACTACA AGTAACATTT ATATATTCTT TTTAAGAAAA 
    TTATGGTGTA ATATTGCAAT GACTAACAAC AAAATTCATT AATTTCGTTT 
    AATTTTCTCA ACAAAACTGT GTAATAAAAT ACAGGAAATC ATATGATATC 
    ATTTTAGTAG ATGAAATTTG GAAATATTCA AAAGTTACTC AACCTCCCCA 
    TAAACAGAAC TTTCCCTCTT CAGGGAAGAA GTATAGGTCC TTCTGGAATA 
    TAATATCATG GTCCACATAG CTCAAATTTA TTCCCACTAT TATCGATTTG 
    ATGTTAACAA CATTAATATC GTCATTGTCA GTAAGTAAGT CCCAAATTTT 
    TTGTCAAGAG TATCATATTT ATATTTTGGT ACAGGGTAGT TTGACTATAG 
    TACCANTACA ACTTGGTCTA TCAAACGAGT ACAAAATTTC TTCATCTGAA 
    AAGGAATGCA TGTGTTAGAA AATAGAGACG NGGAAGTGAA GGAACAA

  • 3 prime edge [+] show sequence

    1. Chromosome: 5; Position: 25645385 [Map]; Strand: c
      • AT5G64070.1 - [+] show detail - phosphatidylinositol 4-OH kinase beta1
        Distance: -939; Region: promoter
      • AT5G64080.1 - [+] show detail - Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfa...
        Distance: 0; Region: exon3 (3UTR)
      • AT5G64080.2 - [+] show detail - Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfa...
        Distance: 0; Region: exon3 (3UTR)
    Flanking Genome Sequence: 53-4456-1 - 3-prime edge 
    ATGAAAACGG TAGAGGTATT TTACCGACCG TTACCGACCG TTTTCATCCG 
    TGCCAAAACA TACATAGATT AACCCTAATA AAAACCATTA ATCAATTTCA 
    TACAGCCAAA TCTAGCTGGT GAAAAAAGAT TCAGTTTTTT TTTTACNCNC 
    TANANAANAA CAAAACGAAC AGTNCCGGCN CTGAGTACAG TTGNGAACGA 
    GGNCGGAATC AAANAANATC CATCGTTCCC TGAANAAGGA TTTGGAGCTA 
    NAAATCCGGC TGTCTCAGGT CCAGCAGCAG CTACTCCTGG AGCAAGACCT 
    GATAAAATTA GGTTCCACAT TAANAAATCT CAAAAGCGTA AAACATNTCA 
    ACGAGAAATC ACATAACTAA CATGGAGAAA AAGAGCAGAG ATTTCCAATC 
    ACTCGAGCAA ATTTTTACAA AGAAAATCAC AAATAAGGAA TT

AT5G64070.1

Model type
Protein coding
Short Description
phosphatidylinositol 4-OH kinase beta1
Curator Summary
Encodes a phosphatidylinositol 4-OH kinase, PI-4Kbeta1. Arabidopsis contains 12 PI-4Ks in three separate families: PI-4Kalphs, PI-4kbeta, and PI-4Kgamma. PI-4Kbeta1 is 83% identical to PI-4kbeta2 encoded by At5g09350. Interacts with the RabA4b GTPase. Important for polarized root hair growth as the loss of this gene and its close relative PI-4kbeta2, leads to the formation of abnormal root hairs.
Computational Description
phosphatidylinositol 4-OH kinase beta1 (PI-4KBETA1); FUNCTIONS IN: 1-phosphatidylinositol 4-kinase activity; INVOLVED IN: phosphoinositide biosynthetic process, root hair cell tip growth, pollen tube growth; LOCATED IN: cytosol, nucleus, membrane; EXPRESSED IN: male gametophyte, root hair tip, cultured cell, pollen tube; EXPRESSED DURING: L mature pollen stage, M germinated pollen stage; CONTAINS InterPro DOMAIN/s: Phosphatidylinositol 3-/4-kinase, catalytic (InterPro:IPR000403), Phosphatidylinositol Kinase (InterPro:IPR015433), Armadillo-type fold (InterPro:IPR016024), Phosphatidylinositol 3/4-kinase, conserved site (InterPro:IPR018936), Protein kinase-like domain (InterPro:IPR011009); BEST Arabidopsis thaliana protein match is: phosphatidylinositol 4-OH kinase beta2 (TAIR:AT5G09350.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT5G64080.1

Model type
Protein coding
Short Description
Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein
Computational Description
Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein; FUNCTIONS IN: lipid binding; INVOLVED IN: lipid transport; LOCATED IN: anchored to plasma membrane, anchored to membrane; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Bifunctional inhibitor/plant lipid transfer protein/seed storage (InterPro:IPR016140), Plant lipid transfer protein/seed storage/trypsin-alpha amylase inhibitor (InterPro:IPR003612), Plant lipid transfer protein/Par allergen (InterPro:IPR000528), Plant lipid transfer protein/hydrophobic protein, helical domain (InterPro:IPR013770); BEST Arabidopsis thaliana protein match is: Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein (TAIR:AT2G13820.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT5G64080.2

Model type
Protein coding
Short Description
Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein
Computational Description
Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein; FUNCTIONS IN: lipid binding; INVOLVED IN: lipid transport; LOCATED IN: anchored to plasma membrane, anchored to membrane; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Bifunctional inhibitor/plant lipid transfer protein/seed storage (InterPro:IPR016140), Plant lipid transfer protein/seed storage/trypsin-alpha amylase inhibitor (InterPro:IPR003612), Plant lipid transfer protein/Par allergen (InterPro:IPR000528), Plant lipid transfer protein/hydrophobic protein, helical domain (InterPro:IPR013770); BEST Arabidopsis thaliana protein match is: Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein (TAIR:AT2G13820.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).
Link
InterPro Scan - TAIR