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Mutant Lines
54-0225-1

54-0225-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

This line has NOT been observed.

Determination of insertion point(s)

5 prime edge [+] show sequence
1. Chromosome: 5; Position: 26452722 [Map]; Strand: w
  - AT5G66190.1 - [+] show detail - ferredoxin-NADP(+)-oxidoreductase 1
    Distance: 0; Region: exon2 (CDS)
  - AT5G66190.2 - [+] show detail - ferredoxin-NADP(+)-oxidoreductase 1
    Distance: 0; Region: exon2 (5UTR)
Flanking Genome Sequence: 54-0225-1 - 5-prime edge
```
AGTGTAAGGG TTCTTAGGTT TGAATTTGTT GACAACAATT CCTTCTTCCT 
GTTTCTTAGA CTCCTTGACT ACTTTAACTG GTGGTGCCTC GGTAGTATCA 
GTTGTCACCT GAGCCTTCAC TGACACAACT CTTCTGTAAC ACACTGTGCT 
CTGTATGCAG GTGCACGAGA TAAATAGCAA TTATGCATGT CAAGATCCAG 
CTTTACAATT AGTGATTAAT TAAAACATGA TAAACGAAAT GGAAATGGAA 
ATGGAAATGA TACCTTCTTG AGGAAAATC
```

3 prime edge [+] show sequence

Chromosome: 5; Position: 26452606 [Map]; Strand: w
- AT5G66190.1 - [+] show detail - ferredoxin-NADP(+)-oxidoreductase 1
  Distance: 0; Region: exon2 (CDS)
- AT5G66190.2 - [+] show detail - ferredoxin-NADP(+)-oxidoreductase 1
  Distance: 0; Region: exon2 (CDS)

CTTACACTGG TCGCTGCCTT TTGAANACCA AGATCACCGG TGATGACGCT 
CCCGGTGAGA CTTGGCACAT TGTCTTCACC ACCGAAGGTA AATCCCTTAA 
TTAGGGGGCT TTTGATTGTA TTTTTTAGAG GCATTTTTCG AGTGAGAGAT 
TTGATTAGGT TGGTTGATGA CTTAGGTGAG GTTCCGTATA GAGAAGGACA 
ATCGATAGGA GTGATTCCAG AGGGAATAGA CAAGAACGGG AAGCCGCACA 
AGCTCAGGCT TTACTCTATC GCCAGTAGTG CCATTGGTGA CTTTGGAGAC 
TCCAAGACCG TAAGCAAATA ATCCCTCTAA CAACTAACTA AATAATGATT 
TACAAAATGA TCTTCTCTTA TCTGGAAATG GATCCTTTTG CAGGTTTCTC 
TCTGTGTCAA GAGACTAGT

AT5G66190.1

Model type: Protein coding
Short Description: ferredoxin-NADP(+)-oxidoreductase 1
Curator Summary: Encodes a leaf-type ferredoxin:NADP(H) oxidoreductase. It is present in both chloroplast stroma and thylakoid membranes but is more abundant in the thylakoid. The affinity of this enzyme for ferredoxin is slightly, but significantly, higher than AtLFNR2, an isoform of the same enzyme. AtLFNR1 forms a heterodimer with AtFNR2 and is also a prerequisite to attach AtFNR2 to the thylakoid membrane.
Computational Description: ferredoxin-NADP(+)-oxidoreductase 1 (FNR1); FUNCTIONS IN: oxidoreductase activity, electron transporter, transferring electrons within the cyclic electron transport pathway of photosynthesis activity, poly(U) RNA binding, NADPH dehydrogenase activity, electron transporter, transferring electrons within the noncyclic electron transport pathway of photosynthesis activity; INVOLVED IN: oxidation reduction, photosynthetic electron transport chain, defense response to bacterium; LOCATED IN: in 7 components; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Oxidoreductase FAD/NAD(P)-binding (InterPro:IPR001433), Ferredoxin reductase-type FAD-binding domain (InterPro:IPR017927), Oxidoreductase, FAD-binding domain (InterPro:IPR008333), Riboflavin synthase-like beta-barrel (InterPro:IPR017938), Ferredoxin Reductase (InterPro:IPR015701), Flavoprotein pyridine nucleotide cytochrome reductase (InterPro:IPR001709), Ferredoxin--NADP reductase (InterPro:IPR012146); BEST Arabidopsis thaliana protein match is: ferredoxin-NADP(+)-oxidoreductase 2 (TAIR:AT1G20020.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).
Link: InterPro Scan - TAIR

AT5G66190.2

Model type: Protein coding
Short Description: ferredoxin-NADP(+)-oxidoreductase 1
Curator Summary: Encodes a leaf-type ferredoxin:NADP(H) oxidoreductase. It is present in both chloroplast stroma and thylakoid membranes but is more abundant in the thylakoid. The affinity of this enzyme for ferredoxin is slightly, but significantly, higher than AtLFNR2, an isoform of the same enzyme. AtLFNR1 forms a heterodimer with AtFNR2 and is also a prerequisite to attach AtFNR2 to the thylakoid membrane.
Computational Description: LEAF FNR 1 (ATLFNR1); FUNCTIONS IN: oxidoreductase activity, electron transporter, transferring electrons within the cyclic electron transport pathway of photosynthesis activity, poly(U) RNA binding, NADPH dehydrogenase activity, electron transporter, transferring electrons within the noncyclic electron transport pathway of photosynthesis activity; INVOLVED IN: oxidation reduction, photosynthetic electron transport chain, defense response to bacterium; LOCATED IN: thylakoid, chloroplast thylakoid membrane, apoplast, chloroplast stroma, chloroplast; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Ferredoxin reductase-type FAD-binding domain (InterPro:IPR017927), Oxidoreductase, FAD-binding domain (InterPro:IPR008333), Ferredoxin--NADP reductase (InterPro:IPR012146), Oxidoreductase FAD/NAD(P)-binding (InterPro:IPR001433), Riboflavin synthase-like beta-barrel (InterPro:IPR017938), Ferredoxin Reductase (InterPro:IPR015701), Flavoprotein pyridine nucleotide cytochrome reductase (InterPro:IPR001709); BEST Arabidopsis thaliana protein match is: ferredoxin-NADP(+)-oxidoreductase 2 (TAIR:AT1G20020.3); Has 7407 Blast hits to 7407 proteins in 1806 species: Archae - 24; Bacteria - 3962; Metazoa - 817; Fungi - 811; Plants - 610; Viruses - 0; Other Eukaryotes - 1183 (source: NCBI BLink).
Link: InterPro Scan - TAIR