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54-1577-2

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

This line has NOT been observed.

Determination of insertion point(s)

  • 5 prime edge [+] show sequence

    1. Chromosome: 5; Position: 1222035 [Map]; Strand: w
      • AT5G04360.1 - [+] show detail - limit dextrinase
        Distance: 0; Region: exon2 (CDS)
    Flanking Genome Sequence: 54-1577-2 - 5-prime edge 
    ATGTNGGTGA AGGGTCTNGN TNCTTTTACN CNAGCNAAAG TGCTGGTTTA 
    TCATTCNGCN AAGATNGANT CNATGGGGCT TAATCTTATC TCTAAGNCNA 
    TGTGATGGCT TGATATTATT TATTGGAAAC CAAGCTTCNT NGGCTTTNAA 
    GNTNTGATCN ANGAATNAAN CTNGAAGCTN AATCCGGTTC ACTTNCTGCA 
    GATGTAAGNT GTGCACTCTC TGCCATGTGT TTGCTTCATA AGAATACATA 
    TTTTTNAGGG AGTTNAGGAA GAAGTNTCAN AGTTGAAGGC TTGATTNCAG 
    GNAATTGAGA AGNNCCCACN TATTNNGAAT TACAAANCGT TCNTAGTTCC 
    TANGGATGNG GATATCAGGG ATCNTGTCCT NGCCGGTNGG NGGCNTNTCC 
    CATNCCCGAT NGGTTTGAGT NAGNGTGTGG ANGNNGNTNA ANTGTGTNGN 
    TCGCNGTTCN NNGNNTCGCC NGNGGGGGGN GGNNCACCCC NNCCCNCCNG 
    GNGTTTCCGN NNGGNNGGGC GTGCNGGNNN GGGGNTCCCT TTTTTTCNCN 
    NCGNTTTGGG GGTNGGGGGG ANNGCCNCCC CAAAGNGNGN GNNGGNGGAA 
    NNCACCCNAC CCCGNCCCCC NCTNTATNGN NTTGCNNTGN TNCNTNAAAN 
    NGTNCNNGNA AATAAANNTC CCCNNCCCCC CCGAGGAAGG GGNNTGGGGN 
    TNAAAANTTT TNGCNNAAAA ACNAAAAANA AAA

  • 3 prime edge [+] show sequence

    1. Chromosome: 5; Position: 1221919 [Map]; Strand: w
      • AT5G04360.1 - [+] show detail - limit dextrinase
        Distance: 0; Region: exon2 (CDS)
    Flanking Genome Sequence: 54-1577-2 - 3-prime edge 
    ACCAACATCC ACATTCCATG CAATCACGCC TTGGGTAACC CAATACGCTC 
    TTGAATAGAT CAAACAATCA AGAAACTGAC CAAAATTTTG ACACAAAACG 
    TTAAAAAGAG CAAAACCAGT AACATGATCG ATCTACCTTC TAGCTCGAAA 
    ATTAAGAGGG GTAAACCTGA GAATTGGGAC TGGAGACTTC GAGCGACATA 
    GGAGATGAAG CGGAAGACGA AGAACAGAGA CAGTGAAGCG AAGTTTTCGA 
    AGGAAGTCTG AGACGGAAGT TAGAAATTGA CGTAACAGGC CGACGTCGCC 
    ACCGCGATCG AAGATTAAAA TCAGCGGCGA AGATCCGAGG ACGCGCGACG 
    GATATGGAAC TGAGAAGATG AACGGAACTT GTTGGTGTCA GTGTCAGTGC 
    CATTCTTCTT CTTCTCCGAT TGATGATAGT TTCAGATGTT CTTCGACCGT 
    TTGTTCTTCA AAATATCTAT ATTTTTGCTC TTTAGTCCCT AAAATTTCTT 
    ATATCTTTCA CGTATTTATA TAACTACTTA TCCTTTCAAA GAAATCCATA 
    TCAATGGAAT GTTTGTTATT AAAGAAAATT TTATCGATAA CTTTATTCAT 
    AATAAGAGGT NCAAAGATCC ACCAAAGAAT ATAAAACCCT AATTTTCATA 
    TGAGGACCAA TTCTTCAGAT CAACCAAAGA TAATACAAAA CTCTAATTTT 
    CAATAAAGN

AT5G04360.1

Model type
Protein coding
Short Description
limit dextrinase
Curator Summary
Encodes an enzyme thought to be involved in the hydrolysis of the α-1,6 linkages during starch degradation in seed endosperm. However, a knockout mutant of Arabidopsis lacking limit dextrinase has normal rates of starch degradation in the leaf at night, indicating that more than one isoamylases might be involved in this process.
Computational Description
limit dextrinase (LDA); FUNCTIONS IN: limit dextrinase activity, pullulanase activity, alpha-amylase activity; INVOLVED IN: starch biosynthetic process, carbohydrate metabolic process, starch catabolic process; LOCATED IN: chloroplast; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Alpha-1,6-glucosidases, pullulanase-type (InterPro:IPR011839), Immunoglobulin E-set (InterPro:IPR014756), Glycoside hydrolase, family 13, N-terminal (InterPro:IPR004193), Glycoside hydrolase, catalytic core (InterPro:IPR017853), Glycoside hydrolase, subgroup, catalytic core (InterPro:IPR013781), Glycosyl hydrolase, family 13, catalytic domain (InterPro:IPR006047); BEST Arabidopsis thaliana protein match is: isoamylase 3 (TAIR:AT4G09020.1); Has 6260 Blast hits to 6253 proteins in 1613 species: Archae - 73; Bacteria - 5462; Metazoa - 45; Fungi - 1; Plants - 262; Viruses - 0; Other Eukaryotes - 417 (source: NCBI BLink).
Link
InterPro Scan - TAIR