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Mutant Lines
54-1994-1

54-1994-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

This line has NOT been observed.

Determination of insertion point(s)

5 prime edge [+] show sequence

Chromosome: 4; Position: 242552 [Map]; Strand: w
- AT4G00560.4 - [+] show detail - NAD(P)-binding Rossmann-fold superfamily protein
  Distance: -201; Region: promoter
- AT4G00560.1 - [+] show detail - NAD(P)-binding Rossmann-fold superfamily protein
  Distance: -173; Region: promoter
- AT4G00560.2 - [+] show detail - NAD(P)-binding Rossmann-fold superfamily protein
  Distance: -173; Region: promoter
- AT4G00560.3 - [+] show detail - NAD(P)-binding Rossmann-fold superfamily protein
  Distance: -173; Region: promoter
- AT4G00570.1 - [+] show detail - NAD-dependent malic enzyme 2
  Distance: 0; Region: exon19 (3UTR)

TTGGTATCAG AACAAAAATG GTAAAGCAGG AAAATGTAGA AGAAAGATAT 
CATTATTGGC TTATTATTGC CAAAACAGTA TATAAAGTTG TAATCATTAT 
CATCAGAAAT AATCGTAAGA AGTTGGAAAA ATGTCCAAAA AAAAAAAGGG 
CCAGGNCCGG GNGNCCCNAA ACCNACCCCN GTNTTNGTNT TNANCCCCTT 
TTTTGGGCTT NGTTTTTTTN AAACCNTNNC CNNTTTGNAA AAAAAAAAAA 
NGGNGGTNCC CAANNAAANN ANNANCCNAA AATTTTTGGN TTTNGGNNTT 
NNTNNNAACC CGGAAAANNN TTNTNNNNNG NGGNGGNNTT TTNNNNGGNA 
NCAANGGGGN NGAAACCGGG AACCCNNNNG NGGGGGGAAN GGAAATCNCC 
GGNNCCCCCC GGGGGGGGGC TTNAAAAAAN CCANTNAANT CCCTTTTTNA 
AANTNCCNTN NCCCNGGTNC GNANCNAAAN NNNNCNCNNN GTCCCTGGNT 
CNANCCCTNA ANNNNGGGGN GNAAANNTTG GGNNCNAANN TNCCNANGNC 
NTTTGGAANG GNNNTNNGCC CCCGANNTTG GNAAANNNGT NNCCCCAATT 
TAAGNNGTTT NGGNCGGGNG GCGGCANTTT TTCNAAAACC NATTGGCTNN 
AAAAACCGGT TTAAATNTCC TAAAATAAAA

3 prime edge [+] show sequence
1. Chromosome: 4; Position: 242488 [Map]; Strand: w
  - AT4G00560.4 - [+] show detail - NAD(P)-binding Rossmann-fold superfamily protein
    Distance: -137; Region: promoter
  - AT4G00560.1 - [+] show detail - NAD(P)-binding Rossmann-fold superfamily protein
    Distance: -109; Region: promoter
  - AT4G00560.2 - [+] show detail - NAD(P)-binding Rossmann-fold superfamily protein
    Distance: -109; Region: promoter
  - AT4G00560.3 - [+] show detail - NAD(P)-binding Rossmann-fold superfamily protein
    Distance: -109; Region: promoter
Flanking Genome Sequence: 54-1994-1 - 3-prime edge
```
ATGAAAACGG TAGAGGTATT TTACCGACCG TTACCGACCG TTTTCATCCC 
TGGATACCAA AAGGTTTAAA TGGTTTCGAG ATTCAACCAA CTTTGAAGAG 
TTGTTAATGC AGTTGCAGAA GCATGTTAAC CGAAAATCAT TCCAAACCGG 
AGAATATCAA CTCGAACCAG AATACCAAAC CGGAAATTCC AACAGTGATC 
GTGCTATGTT TAAGTTAAAA TTTAAATTAA GCAAACACCG GAGAGTGATT 
GGATCGGGGA ATTTGGGAAA AGTATGGAGA AGAAGACGAA AGTGCTAATT 
GTGGGAGGTA CTGGATACTT GGGTCAGCAT CTTCTTCAAG CT
```

AT4G00560.1

Model type: Protein coding
Short Description: NAD(P)-binding Rossmann-fold superfamily protein
Computational Description: NAD(P)-binding Rossmann-fold superfamily protein; FUNCTIONS IN: dTDP-4-dehydrorhamnose reductase activity, binding, catalytic activity; INVOLVED IN: dTDP-rhamnose biosynthetic process, extracellular polysaccharide biosynthetic process, metabolic process; LOCATED IN: endomembrane system; EXPRESSED IN: 17 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: NAD(P)-binding domain (InterPro:IPR016040), dTDP-4-dehydrorhamnose reductase (InterPro:IPR005913); Has 7761 Blast hits to 7761 proteins in 1842 species: Archae - 350; Bacteria - 5037; Metazoa - 116; Fungi - 124; Plants - 45; Viruses - 0; Other Eukaryotes - 2089 (source: NCBI BLink).
Link: InterPro Scan - TAIR

AT4G00560.2

Model type: Protein coding
Short Description: NAD(P)-binding Rossmann-fold superfamily protein
Computational Description: NAD(P)-binding Rossmann-fold superfamily protein; FUNCTIONS IN: dTDP-4-dehydrorhamnose reductase activity, binding, catalytic activity; INVOLVED IN: extracellular polysaccharide biosynthetic process, metabolic process; LOCATED IN: endomembrane system; EXPRESSED IN: 17 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: NAD(P)-binding domain (InterPro:IPR016040), dTDP-4-dehydrorhamnose reductase (InterPro:IPR005913); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).
Link: InterPro Scan - TAIR

AT4G00560.3

Model type: Protein coding
Short Description: NAD(P)-binding Rossmann-fold superfamily protein
Computational Description: NAD(P)-binding Rossmann-fold superfamily protein; FUNCTIONS IN: dTDP-4-dehydrorhamnose reductase activity, binding, catalytic activity; INVOLVED IN: extracellular polysaccharide biosynthetic process, metabolic process; LOCATED IN: endomembrane system; EXPRESSED IN: 17 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: NAD(P)-binding domain (InterPro:IPR016040), dTDP-4-dehydrorhamnose reductase (InterPro:IPR005913); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).
Link: InterPro Scan - TAIR

AT4G00560.4

Model type: Protein coding
Short Description: NAD(P)-binding Rossmann-fold superfamily protein
Computational Description: NAD(P)-binding Rossmann-fold superfamily protein; FUNCTIONS IN: dTDP-4-dehydrorhamnose reductase activity, binding, catalytic activity; INVOLVED IN: extracellular polysaccharide biosynthetic process, metabolic process; LOCATED IN: endomembrane system; EXPRESSED IN: 17 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: NAD(P)-binding domain (InterPro:IPR016040), dTDP-4-dehydrorhamnose reductase (InterPro:IPR005913).
Link: InterPro Scan - TAIR

AT4G00570.1

Model type: Protein coding
Short Description: NAD-dependent malic enzyme 2
Curator Summary: Encodes an NAD-dependent malic enzyme (NAD-ME) that does not act on oxaloacetate, indicating that it belongs to EC 1.1.1.39. It is a member of the beta family of NAD-MEs in plants. It appears to function as a homodimer or as a heterodimer with the alpha-type NAD-ME2 (At2g13560). NAD-ME2 transcript and protein levels are higher during the night than during the day.
Computational Description: NAD-dependent malic enzyme 2 (NAD-ME2); FUNCTIONS IN: in 7 functions; INVOLVED IN: malate metabolic process; LOCATED IN: mitochondrion, chloroplast; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Malic enzyme, NAD-binding (InterPro:IPR012302), Malic oxidoreductase (InterPro:IPR001891), Malic enzyme, conserved site (InterPro:IPR015884), Malic enzyme, N-terminal (InterPro:IPR012301), NAD(P)-binding domain (InterPro:IPR016040); BEST Arabidopsis thaliana protein match is: NAD-dependent malic enzyme 1 (TAIR:AT2G13560.1); Has 9343 Blast hits to 9326 proteins in 2439 species: Archae - 143; Bacteria - 6323; Metazoa - 607; Fungi - 223; Plants - 463; Viruses - 0; Other Eukaryotes - 1584 (source: NCBI BLink).
Link: InterPro Scan - TAIR