1. Home
  2. Mutant Lines
  3. 54-2387-1

54-2387-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

This line has NOT been observed.

Determination of insertion point(s)

  • 3 prime edge [+] show sequence

    1. Chromosome: 3; Position: 18003564 [Map]; Strand: w
      • AT3G48560.1 - [+] show detail - chlorsulfuron/imidazolinone resistant 1
        Distance: 0; Region: exon1 (5UTR)
    Flanking Genome Sequence: 54-2387-1 - 3-prime edge
    TCCCCGGGTA CTCGAGCTCA TTTCTCTATT ACTTCAGCCA TAACAAAAGA 
    ACTCTTTTCT CTTCTTATTA AACCATGGCG GCGGCAACAA CAACAACAAC
    AACATCTTCT TCGATCTCCT TCTCCACCAA ACCATCTCCT TCCTCCTCCA
    AATCACCATT ACCAATCTCC AGATTCTCCC TCCCATTCTC CCTAAACCCC
    AACAAATCAT CCTCCTCCTC CCGCCGCCGC GGTATCAAAT CCAGCTCTCC
    CTCCTCCATC TCCGCCGTGC TCAACACAAC CACCAATGTC ACAACCACTC
    CCTCTCCAAC CAAACCTACC AAACCCGAAA CATTCATCTC CCGATTCGCT
    CCAGATCAAC CCCGCAAAGG CGCTGATATC CTCGTCGAAG CT

AT3G48560.1

Model type
Protein coding
Short Description
chlorsulfuron/imidazolinone resistant 1
Curator Summary
Catalyzes the formation of acetolactate from pyruvate, the first step in valine and isoleucine biosynthesis. Requires FAD, thiamine pyrophosphate and Mg. Inhibited by the sulphonylurea herbicide, chlorsulphuron, and the imidazolinone herbicide, imazapyr. The obtained crystal structure of acetohydroxyacid synthase AHAS, EC 2.2.1.6)in complex with herbicides of the sulphonylurea and imidazolinone family reveals the molecular basis for substrate/inhibitor binding.
Computational Description
chlorsulfuron/imidazolinone resistant 1 (CSR1); FUNCTIONS IN: acetolactate synthase activity, pyruvate decarboxylase activity; INVOLVED IN: branched chain family amino acid biosynthetic process; LOCATED IN: chloroplast; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: TPP-binding enzyme, conserved site (InterPro:IPR000399), Thiamine pyrophosphate enzyme, central domain (InterPro:IPR012000), Acetolactate synthase, large subunit, biosynthetic (InterPro:IPR012846), Thiamine pyrophosphate enzyme, N-terminal TPP-binding domain (InterPro:IPR012001), Thiamine pyrophosphate enzyme, C-terminal TPP-binding (InterPro:IPR011766); BEST Arabidopsis thaliana protein match is: Thiamine pyrophosphate dependent pyruvate decarboxylase family protein (TAIR:AT5G17380.1); Has 30963 Blast hits to 30605 proteins in 2694 species: Archae - 488; Bacteria - 17421; Metazoa - 266; Fungi - 871; Plants - 599; Viruses - 25; Other Eukaryotes - 11293 (source: NCBI BLink).
Link
InterPro Scan - TAIR