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54-3294-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

This line has NOT been observed.

Determination of insertion point(s)

  • 5 prime edge [+] show sequence

    1. Chromosome: 5; Position: 26452600 [Map]; Strand: w
      • AT5G66190.1 - [+] show detail - ferredoxin-NADP(+)-oxidoreductase 1
        Distance: 0; Region: exon2 (CDS)
      • AT5G66190.2 - [+] show detail - ferredoxin-NADP(+)-oxidoreductase 1
        Distance: 0; Region: exon2 (CDS)
    Flanking Genome Sequence: 54-3294-1 - 5-prime edge
    CNTGANAAAA CTAACAAAAT CGGTTATACG ATAACGGTCG GTACGGGATT 
    TTCCCATCCT ACTTTCATCC CCTCACCGGG AGCGTCATCA CCGGTGATCT
    TGGTGTTCAA AAGGCAGCGA CCAGTGTAAG GGTTCTTAGG TTTGAATTTG
    TTGACAACAA TTCCTTCTTC CTGTTTCTTA GACTCCTTGA CTACTTTAAC
    NTGGTGGTGC CTCGGTAGTA TCAGTTGTCA CCTGAGCCTT CACTGACACA
    ACTCTTCTGT AACACACTGT GCTCTGTATG CAGGTGCACG AGATAAATAG
    CAATTATGCA TGTCAAGATC CAGCTTTACA ATTAGTGATT AATTAAAACA
    TGATAAACGA AATGGAAATG GAAATGGAAA TGATACCTTC TTGAGGAAAA
    TCCTTTGAGG GGATACAGAG GAGATTTTGG TGGGGAGAGA GGATGACTTG
    GAGGAAGGTA AAGAGACTGC AGCACTTATA GCAGCAGCCA TGGTTGATCA
    GAAGAAGAAG AAGAAGATGA ACTGATGGGA GATCAAAGTC AAAAAAATAA
    TAAGCAAAAA AAAAANNNTC AAAAAAAAAA AANTTACCGG NAAATNTNTA
    ATNAGGTTGG AATTGGATGT GNGNGGATTG NGTAAAGGGC ANTGNGAGGC
    CAAGTNTTAA AAAGGATAAN TNNTTNCTGC TTATNATTGC CCTAATTNAC
    CCTTTCNTGC CCCTGTNGTN CCATTTNANC CCTACTTNAA AAAAAAANTG
    NTNAAANTTN CTTTTNAAAN TNNG
  • 3 prime edge [+] show sequence

    1. Chromosome: 5; Position: 26452553 [Map]; Strand: w
      • AT5G66190.1 - [+] show detail - ferredoxin-NADP(+)-oxidoreductase 1
        Distance: 0; Region: intron2 (CDS)
      • AT5G66190.2 - [+] show detail - ferredoxin-NADP(+)-oxidoreductase 1
        Distance: 0; Region: intron2 (CDS)
    Flanking Genome Sequence: 54-3294-1 - 3-prime edge
    GGTGAGACTT GGCACATTGT CTTCACCACC GAAGGTAAAT CCCTTAATTA 
    GGGGGCTTTT GATTGTATTT TTTAGAGGCA TTTTTCGAGT GAGAGATTTG
    ATTAGGTTGG TTGATGACTT AGGTGAGGTT CCGTATAGAG AAGGACAATC
    GATAGGAGTG ATTCCAGAGG GAATAGACAA GAACGGGAAG CCGCACAAGC
    TCAGGCTTTA CTCTATCGCC AGTAGTGCCA TTGGTGACTT TGGAGACTCC
    AAGACCGTAA GCAAATAATC CCTCTAACAA CTAACTAAAT AATGATTTAC
    AAAATGATCT TCTCTTATCT GGAAATGGAT C

AT5G66190.1

Model type
Protein coding
Short Description
ferredoxin-NADP(+)-oxidoreductase 1
Curator Summary
Encodes a leaf-type ferredoxin:NADP(H) oxidoreductase. It is present in both chloroplast stroma and thylakoid membranes but is more abundant in the thylakoid. The affinity of this enzyme for ferredoxin is slightly, but significantly, higher than AtLFNR2, an isoform of the same enzyme. AtLFNR1 forms a heterodimer with AtFNR2 and is also a prerequisite to attach AtFNR2 to the thylakoid membrane.
Computational Description
ferredoxin-NADP(+)-oxidoreductase 1 (FNR1); FUNCTIONS IN: oxidoreductase activity, electron transporter, transferring electrons within the cyclic electron transport pathway of photosynthesis activity, poly(U) RNA binding, NADPH dehydrogenase activity, electron transporter, transferring electrons within the noncyclic electron transport pathway of photosynthesis activity; INVOLVED IN: oxidation reduction, photosynthetic electron transport chain, defense response to bacterium; LOCATED IN: in 7 components; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Oxidoreductase FAD/NAD(P)-binding (InterPro:IPR001433), Ferredoxin reductase-type FAD-binding domain (InterPro:IPR017927), Oxidoreductase, FAD-binding domain (InterPro:IPR008333), Riboflavin synthase-like beta-barrel (InterPro:IPR017938), Ferredoxin Reductase (InterPro:IPR015701), Flavoprotein pyridine nucleotide cytochrome reductase (InterPro:IPR001709), Ferredoxin--NADP reductase (InterPro:IPR012146); BEST Arabidopsis thaliana protein match is: ferredoxin-NADP(+)-oxidoreductase 2 (TAIR:AT1G20020.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT5G66190.2

Model type
Protein coding
Short Description
ferredoxin-NADP(+)-oxidoreductase 1
Curator Summary
Encodes a leaf-type ferredoxin:NADP(H) oxidoreductase. It is present in both chloroplast stroma and thylakoid membranes but is more abundant in the thylakoid. The affinity of this enzyme for ferredoxin is slightly, but significantly, higher than AtLFNR2, an isoform of the same enzyme. AtLFNR1 forms a heterodimer with AtFNR2 and is also a prerequisite to attach AtFNR2 to the thylakoid membrane.
Computational Description
LEAF FNR 1 (ATLFNR1); FUNCTIONS IN: oxidoreductase activity, electron transporter, transferring electrons within the cyclic electron transport pathway of photosynthesis activity, poly(U) RNA binding, NADPH dehydrogenase activity, electron transporter, transferring electrons within the noncyclic electron transport pathway of photosynthesis activity; INVOLVED IN: oxidation reduction, photosynthetic electron transport chain, defense response to bacterium; LOCATED IN: thylakoid, chloroplast thylakoid membrane, apoplast, chloroplast stroma, chloroplast; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Ferredoxin reductase-type FAD-binding domain (InterPro:IPR017927), Oxidoreductase, FAD-binding domain (InterPro:IPR008333), Ferredoxin--NADP reductase (InterPro:IPR012146), Oxidoreductase FAD/NAD(P)-binding (InterPro:IPR001433), Riboflavin synthase-like beta-barrel (InterPro:IPR017938), Ferredoxin Reductase (InterPro:IPR015701), Flavoprotein pyridine nucleotide cytochrome reductase (InterPro:IPR001709); BEST Arabidopsis thaliana protein match is: ferredoxin-NADP(+)-oxidoreductase 2 (TAIR:AT1G20020.3); Has 7407 Blast hits to 7407 proteins in 1806 species: Archae - 24; Bacteria - 3962; Metazoa - 817; Fungi - 811; Plants - 610; Viruses - 0; Other Eukaryotes - 1183 (source: NCBI BLink).
Link
InterPro Scan - TAIR