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54-3369-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

This line has NOT been observed.

Determination of insertion point(s)

  • 5 prime edge [+] show sequence

    1. Chromosome: 5; Position: 26940430 [Map]; Strand: w
      • AT5G67520.1 - [+] show detail - adenosine-5'-phosphosulfate (APS) kinase 4
        Distance: 0; Region: intron4 (CDS)
      • AT5G67530.1 - [+] show detail - plant U-box 49
        Distance: -752; Region: promoter
    Flanking Genome Sequence: 54-3369-1 - 5-prime edge
    NNTTATANNA NCGTGCGCAG CNCTGGTNAA ANTANANGTA ACTTTGNNNT 
    GACTCTGCNN GNGAANCNAN CTTANCCNAN GTGTCTACNG NTCNTGCTTA
    NTTATATAAC CTTCTTGATC TATCCATTTG ATTATATGTA AATATATTGT
    TCCCCAAAAC ATTTCTTCTC CAGGTTTTAC AGGAGTAGAT GATCCATATG
    AAGCGCCTTT GGATTGCGAG GTTCATATAA TATCCAATTT TTGTTATATA
    AGTTGGAATC TAGCTCATCT CCGAACTTGA ATCTAATGTT GTGTTTCCGA
    CTACTCTTTG CAGATTGTAA TACAAAACAG TAGAGACAAG GGGCTTTCTT
    CTTCATCTTC ATCTTCATCT TCACCTTCAT CTTCGTCTTC TTCTCTGTGT
    GAAATGGCAG ATATTGTTGT GTCGTACTTG GACCAAAATG GATACCTGAA
    GAAACACTCC ACAAAATCAC GTGATTGTAT GTAAATGTAA TATTAATATC
    ATTGTTGTAA CGTTTCTTAA ACCTATTTTA CCTATGGACG ATTAAATTGT
    GAAAACATAA ATGTAATTCA CAATCTGCAA AGAGTTGGTA AGAAAAACTT
    GTTTTATTGA TTGAGAGAAT GAGATTATTA TATGGCCGCC AAGTTGGCGC
    CAACTCTCCG GCTTTTTAAG TAATCGAGCA TTTGGGCCTA
  • 3 prime edge [+] show sequence

    1. Chromosome: 5; Position: 26940316 [Map]; Strand: w
      • AT5G67520.1 - [+] show detail - adenosine-5'-phosphosulfate (APS) kinase 4
        Distance: 0; Region: exon4 (CDS)
      • AT5G67530.1 - [+] show detail - plant U-box 49
        Distance: -866; Region: promoter
    Flanking Genome Sequence: 54-3369-1 - 3-prime edge
    GTATAAGCCC TTTGGATCTC TAGCTTCACA AACATGGAGT GGCACATCCA 
    TAAATACCTA TCAAGAGAAC CAAGAGAAAG TCAGATTGAT GTTGAGACTG
    GGATTCAAAT TCATGAACTT GGAGAAACCT CAATGAAATC TCCTTGTGGT
    AATAATGCAC GGCAGGCAGC TCGTTCTATC CGGTAAGGAG ATATTAAACT
    TGCAATACAG ATAATACCAG AATCTGCAAA CAGTTTAGCC ACTTCACCAA
    CTCTTCGAAT GTTTTCAGCT CGATCATCTG CTTCGAAACT AAGATCGCTG
    TTTAAACCAT GTCGAACATT GTCACCATCA AGTATATACG AAAGCT

AT5G67520.1

Model type
Protein coding
Short Description
adenosine-5'-phosphosulfate (APS) kinase 4
Curator Summary
Provides activated sulfate for the sulfation of secondary metabolites, including the glucosinolates. Redundant with APK3.
Computational Description
adenosine-5'-phosphosulfate (APS) kinase 4 (APK4); FUNCTIONS IN: kinase activity, transferase activity, transferring phosphorus-containing groups, ATP binding; INVOLVED IN: male gamete generation, sulfate assimilation; LOCATED IN: chloroplast, plastid; EXPRESSED IN: 18 plant structures; EXPRESSED DURING: L mature pollen stage, M germinated pollen stage, 4 anthesis, petal differentiation and expansion stage, E expanded cotyledon stage; CONTAINS InterPro DOMAIN/s: Adenylylsulphate kinase, C-terminal (InterPro:IPR002891); BEST Arabidopsis thaliana protein match is: APS kinase (TAIR:AT2G14750.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT5G67530.1

Model type
Protein coding
Short Description
plant U-box 49
Computational Description
plant U-box 49 (PUB49); FUNCTIONS IN: ubiquitin-protein ligase activity, peptidyl-prolyl cis-trans isomerase activity; INVOLVED IN: protein folding, protein ubiquitination; LOCATED IN: ubiquitin ligase complex; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Cyclophilin-like (InterPro:IPR015891), Peptidyl-prolyl cis-trans isomerase, cyclophilin-type (InterPro:IPR002130), U box domain (InterPro:IPR003613), Peptidyl-prolyl cis-trans isomerase, cyclophilin-type, conserved site (InterPro:IPR020892); BEST Arabidopsis thaliana protein match is: Cyclophilin-like peptidyl-prolyl cis-trans isomerase family protein (TAIR:AT1G01940.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).
Link
InterPro Scan - TAIR