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54-3962-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

This line has NOT been observed.

Determination of insertion point(s)

  • 5 prime edge [+] show sequence

    1. Chromosome: 3; Position: 22766702 [Map]; Strand: w
      • AT3G61510.1 - [+] show detail - ACC synthase 1
        Distance: -972; Region: promoter
    Flanking Genome Sequence: 54-3962-1 - 5-prime edge
    GNGAAAAACT AACAAAATCG GTTATACGAT AACGGTCGGT ACGGGATTTT 
    CCCATCCTAC TTTCATCCAT GGGTTGGTCG TTGGGTTTAG CTCCAAAGAG
    TTCTTCAAGG TCATTCATTA TGTTTATAGT AATCCAACAA AATTAACATG
    TGAAAAAGTA TTGACACGTG GAGCAGCCCA CTGAATTGGC GATCAAAGAT
    CTTTTTGGGC NGGCTTTCTA GATTTACGAC ATAGTAAAAC TCTCTCTACA
    TTAATCTTTG CCTTAGATTG AATTGGTCGG AGATCGAAAA GACTAAAACT
    AGATGGACAC GCTGTATGTA TCTAAGTGGA GTGATTACTC GTTTTCTTAG
    AGTGTATTTG TAGGGAAAAA GAATACGTAT ACGCACGTAC AGTTGTTAGG
    GGTCGGATTG GGCCTACTTG GCCCAATGCC CAAACCCAAG TCAAAAACCC
    AAAATAAGTT CCAAACCAAG GAGTATTAAG GCGAAGGGTA GTTTCGTCAA
    ATCCCTCGAT GAATGCCCTA CACGCATAGT GCTATAAATA CCGAATAGCA
    TGTCTTGTCA CGAGGGGGGA TTCCGCCAGG ACACTTAGAG ACAAAGAATA
    GCCCGTCTCA AGTAGCTCGT CAGCTCACCC TTCTCAAGTA GCTCGTCCGT
    CTCAAGTCGC TTGTCTCGCC CTTCTCAAGT AGCTCGTCCG TCTAAAGTAG
    CTTGTCAGCT CGCCCTTCTC AAGAAGCTCG TTAGCTCGCT CCTCATCGCC
    NAACTCTTCA GCTCGCCTTT AAGCTCTTCA AGCTCCTCGG
  • 3 prime edge [+] show sequence

    1. Chromosome: 3; Position: 22766698 [Map]; Strand: w
      • AT3G61510.1 - [+] show detail - ACC synthase 1
        Distance: -968; Region: promoter
    Flanking Genome Sequence: 54-3962-1 - 3-prime edge
    GAANACGGTA GAGGTATTTT ACCGACCGTT ACCGACCGTT TTCATCCCCC 
    ATTACGGCAT TACCATTCTC TCTACATGAT TTTCTTCCTC TTAAACTAAT
    TTAAAATGGA GAAAGACAAA ATTTGAAATT TTATTTTCAC GTGATTAGAC
    CATGATTACG TTTCGGTCGA CACGCAAGTA ATTTTAAAAC TTAAAACATA
    TGATTTGAAA AAGGTAAAAC ACCAAGTCAT GGTAAACATC TCCCCCCGAA
    TCTTATATCA ACGTGGGTCT AATAATAGAG TTCGTAAGAC TTGAACTTGT
    TAATTTGTTA CTCCGTTGAA TCTTTAAAAC ACTCTCGACT TTTAATTTGT
    TTTTGTCTGT AAAACCTCTT GCTTGATCTA ATTGTTTAAC TAAAATTAAT
    GAAACACAAA GTCTTTTTAA TGTAATTAGT ACTTAGTTAT TACCGTGATA
    CCAAGTCGCG GCTGTATTTT TAACACAAAG TATACTTTGT CTTACAATGT
    TTTTCATAGT ATATAAAGTT CTTAAATTTT CAAATGTACC ATCTGCATGG
    TACAGGTGTG TCTAAAAAAA TCTACAGTTG TTTATCATCA TTAATTCTTA
    TTTATTTTTA TATCAAAACT ATAATTTTTA GTTACAGAAA TTAAATGTTT
    GTTCATTTGT TGATTATAGA TACTCATAAA TATTAATAAG ACTTTTTTTT
    GGCCATATAA CAGTTCCGTT GTAAAAAAAA AATGTTNTAA TTGGCNTAGA
    TGAAGAAAAA AAAAGACTNC CANCNT

AT3G61510.1

Model type
Protein coding
Short Description
ACC synthase 1
Curator Summary
Encodes a member of the 1-aminocyclopropane-1-carboxylate (ACC) synthase (S-adenosyl-L-methionine methylthioadenosine-lyase, EC 4.4.1.14) gene family. The gene is transcriptionally active but enzymatically inactive. The predicted amino-acid sequence of ACS1 is missing the highly conserved tripeptide, Thr-Asn-Pro (TNP), between Ile204 and Ser205. Introduction of TNP into ACS1 restores the ACS activity.
Computational Description
ACC synthase 1 (ACS1); CONTAINS InterPro DOMAIN/s: 1-aminocyclopropane-1-carboxylate synthase (InterPro:IPR001176), Pyridoxal phosphate-dependent transferase, major domain (InterPro:IPR015424), Aminotransferase, class I/classII (InterPro:IPR004839), Aminotransferases, class-I, pyridoxal-phosphate-binding site (InterPro:IPR004838), Pyridoxal phosphate-dependent transferase, major region, subdomain 1 (InterPro:IPR015421), Pyridoxal phosphate-dependent transferase, major region, subdomain 2 (InterPro:IPR015422); BEST Arabidopsis thaliana protein match is: 1-amino-cyclopropane-1-carboxylate synthase 2 (TAIR:AT1G01480.1); Has 22999 Blast hits to 22998 proteins in 2818 species: Archae - 808; Bacteria - 15898; Metazoa - 560; Fungi - 631; Plants - 1241; Viruses - 0; Other Eukaryotes - 3861 (source: NCBI BLink).
Link
InterPro Scan - TAIR