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Mutant Lines
54-4347-1

54-4347-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

This line has NOT been observed.

Determination of insertion point(s)

5 prime edge [+] show sequence
1. Chromosome: 5; Position: 26284685 [Map]; Strand: c
  - AT5G65700.2 - [+] show detail - Leucine-rich receptor-like protein kinase family protein
    Distance: 0; Region: exon2 (CDS)
  - AT5G65700.1 - [+] show detail - Leucine-rich receptor-like protein kinase family protein
    Distance: 0; Region: exon2 (CDS)
Flanking Genome Sequence: 54-4347-1 - 5-prime edge
```
GAAGACGTGC GTCACTTCAT GAATCGGAAT CGAAGAAAGT CTCGGATC
```

3 prime edge [+] show sequence

Chromosome: 5; Position: 26284801 [Map]; Strand: c
- AT5G65700.2 - [+] show detail - Leucine-rich receptor-like protein kinase family protein
  Distance: 0; Region: exon2 (CDS)
- AT5G65700.1 - [+] show detail - Leucine-rich receptor-like protein kinase family protein
  Distance: 0; Region: exon2 (CDS)

NCGTCTTCTN TGTAGCGATG CTCTGTGTAG ANGAACANGC TGTTGANAGG 
CCGACTATGA NAGAAGNTGT TCAGATTCTC ACTGAGATCC CGAAGTTGCC 
ACCGTCNAAG GATCAGCCGA TGACGGAATC AGCGCCGGAG AGTGAGCTTT 
CNCCGAAGTC TGGTGTTCAA AGTCCNNCNG ATCTACTCAA TCTATGAGAG 
TTTTCTGATT CACGGCGGTT AATTTAGGGT ACCGGTATAA GGGGTAAATT 
TGTCTTTTCA TAAATGTTAT TAGCGATGAG ATTNGGTTGT TAATTTACTT 
TAATCCCTCT TAGTACGGTT TGTGCTTTGG TCCGGTTTAG TAGGTTTTTT 
TTTGAAAGAA GGTTGTTTAA ACTCTTTTTA AGNGNGTTTC ATTNTCCTTT 
TTNATGTTTT TAATCGGNAT TNGGCTTTNT TTAGTGGGCA ATNCGGGGTT 
ANNGAAAANG NNNCNGTGGG TTTTTNTTTT GAAGGGANNT NTCAAAATTC 
TTNTNGATAA AGAGNGAATG GGGGTANCGG NNNANTCCCC TTNGCATGGT 
NTNGCTTTNG AGCCCNCCCA TTCGCNANCT NGTTNTNANC NNACTCANCT 
TNTTTANTNG NTTTTANGNN TTNCCNCNTT TNNNGCCCTN NGATTNNAAN 
TGNNGGTTTC TACCCCCCNT CGTNNCCNNN NAAAAANATT TCGCCGNGNC 
NNGANATCTT NTTNTANTTT CCTCCTCAGC GAGNNTGNGA NAGNGGTTAA 
AAGNGAAAGA NANNAANNAN NNANNAG

AT5G65700.1

Model type: Protein coding
Short Description: Leucine-rich receptor-like protein kinase family protein
Curator Summary: Encodes a CLAVATA1-related receptor kinase-like protein required for both shoot and flower meristem function. Very similar to BAM2,with more than 85% a.a. identity. It has a broad expression pattern and is involved in vascular strand development in the leaf, control of leaf shape, size and symmetry, male gametophyte development and ovule specification and function. Anthers of double mutants (bam1bam2) appeared abnormal at a very early stage and lack the endothecium, middle, and tapetum layers. Further analyses revealed that cells interior to the epidermis (in anther tissue) acquire some characteristics of pollen mother cells (PMCs), suggesting defects in cell fate specification. The pollen mother-like cells degenerate before the completion of meiosis, suggesting that these cells are defective. In addition, the BAM1 expression pattern supports both an early role in promoting somatic cell fates and a subsequent function in the PMCs.
Computational Description: BARELY ANY MERISTEM 1 (BAM1); FUNCTIONS IN: protein serine/threonine kinase activity, kinase activity, ATP binding; INVOLVED IN: in 9 processes; LOCATED IN: plasma membrane; EXPRESSED IN: 32 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Protein kinase, catalytic domain (InterPro:IPR000719), Leucine-rich repeat-containing N-terminal domain, type 2 (InterPro:IPR013210), Leucine-rich repeat (InterPro:IPR001611), Serine/threonine-protein kinase-like domain (InterPro:IPR017442), Protein kinase-like domain (InterPro:IPR011009), Serine/threonine-protein kinase, active site (InterPro:IPR008271); BEST Arabidopsis thaliana protein match is: Leucine-rich receptor-like protein kinase family protein (TAIR:AT3G49670.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).
Link: InterPro Scan - TAIR

AT5G65700.2

Model type: Protein coding
Short Description: Leucine-rich receptor-like protein kinase family protein
Curator Summary: Encodes a CLAVATA1-related receptor kinase-like protein required for both shoot and flower meristem function. Very similar to BAM2,with more than 85% a.a. identity. It has a broad expression pattern and is involved in vascular strand development in the leaf, control of leaf shape, size and symmetry, male gametophyte development and ovule specification and function. Anthers of double mutants (bam1bam2) appeared abnormal at a very early stage and lack the endothecium, middle, and tapetum layers. Further analyses revealed that cells interior to the epidermis (in anther tissue) acquire some characteristics of pollen mother cells (PMCs), suggesting defects in cell fate specification. The pollen mother-like cells degenerate before the completion of meiosis, suggesting that these cells are defective. In addition, the BAM1 expression pattern supports both an early role in promoting somatic cell fates and a subsequent function in the PMCs.
Computational Description: BARELY ANY MERISTEM 1 (BAM1); CONTAINS InterPro DOMAIN/s: Protein kinase, catalytic domain (InterPro:IPR000719), Leucine-rich repeat-containing N-terminal domain, type 2 (InterPro:IPR013210), Leucine-rich repeat (InterPro:IPR001611), Serine/threonine-protein kinase-like domain (InterPro:IPR017442), Protein kinase-like domain (InterPro:IPR011009), Serine/threonine-protein kinase, active site (InterPro:IPR008271); BEST Arabidopsis thaliana protein match is: Leucine-rich receptor-like protein kinase family protein (TAIR:AT3G49670.1).
Link: InterPro Scan - TAIR