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54-4574-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

This line has NOT been observed.

Determination of insertion point(s)

  • 5 prime edge [+] show sequence

    1. Chromosome: 5; Position: 26453862 [Map]; Strand: c
      • AT5G66190.1 - [+] show detail - ferredoxin-NADP(+)-oxidoreductase 1
        Distance: -663; Region: promoter
      • AT5G66190.2 - [+] show detail - ferredoxin-NADP(+)-oxidoreductase 1
        Distance: -663; Region: promoter
      • AT5G66200.1 - [+] show detail - armadillo repeat only 2
        Distance: 0; Region: exon1 (CDS)
    Flanking Genome Sequence: 54-4574-1 - 5-prime edge
    CTCGAGGGGC ATTATAGAAG CTGGAGGGGG CAAACATCTG GTTCAGTTGG 
    CCTACTTTGG AGAGGGTGGC GTACAGATTC CGGCATTAGA GCTCCTCTGT
    TACATAGCAC TCAATGTCCC AGACAGCGAA CAGCTGGCCA AGGACGAGGT
    TCTGGCAGTG TTGGAATGGG CTTCGAAGCA GTCATGGGTA ACGCAGCTGG
    AGAGCTTAGA AGCTNCCANC CCGNNCCGTA AACCACNCTT GCCCCTNTNG
    ATTNGANTNT NACNAGATGG NNNCGNGTAT TAACTGCNTT TAAGANCTTA
    TNNCGGNTCA AGNTCTTATA CNCAATCNTA CACATNACAT CAAGTNATAT
    NCAACTCCAA AACNCTAACC AAGACTTCTG GCTNNNNNCT GAAAGCTACC
    ANCCCCGGGC CGNNNACCAC NCNTNNNNCT ATA
  • 3 prime edge [+] show sequence

    1. Chromosome: 5; Position: 26453978 [Map]; Strand: c
      • AT5G66190.1 - [+] show detail - ferredoxin-NADP(+)-oxidoreductase 1
        Distance: -779; Region: promoter
      • AT5G66190.2 - [+] show detail - ferredoxin-NADP(+)-oxidoreductase 1
        Distance: -779; Region: promoter
      • AT5G66200.1 - [+] show detail - armadillo repeat only 2
        Distance: 0; Region: exon1 (CDS)
    Flanking Genome Sequence: 54-4574-1 - 3-prime edge
    CCCTCGAGTG ATCTTTGTGC AAGTAATTCG CCGTGCAGGC AAACTTCGTG 
    AGGGCAGCCG CAGCTTCCCC TGTCACTTCG GGCTCTCGTT CGTCCAGAAG
    CT

AT5G66190.1

Model type
Protein coding
Short Description
ferredoxin-NADP(+)-oxidoreductase 1
Curator Summary
Encodes a leaf-type ferredoxin:NADP(H) oxidoreductase. It is present in both chloroplast stroma and thylakoid membranes but is more abundant in the thylakoid. The affinity of this enzyme for ferredoxin is slightly, but significantly, higher than AtLFNR2, an isoform of the same enzyme. AtLFNR1 forms a heterodimer with AtFNR2 and is also a prerequisite to attach AtFNR2 to the thylakoid membrane.
Computational Description
ferredoxin-NADP(+)-oxidoreductase 1 (FNR1); FUNCTIONS IN: oxidoreductase activity, electron transporter, transferring electrons within the cyclic electron transport pathway of photosynthesis activity, poly(U) RNA binding, NADPH dehydrogenase activity, electron transporter, transferring electrons within the noncyclic electron transport pathway of photosynthesis activity; INVOLVED IN: oxidation reduction, photosynthetic electron transport chain, defense response to bacterium; LOCATED IN: in 7 components; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Oxidoreductase FAD/NAD(P)-binding (InterPro:IPR001433), Ferredoxin reductase-type FAD-binding domain (InterPro:IPR017927), Oxidoreductase, FAD-binding domain (InterPro:IPR008333), Riboflavin synthase-like beta-barrel (InterPro:IPR017938), Ferredoxin Reductase (InterPro:IPR015701), Flavoprotein pyridine nucleotide cytochrome reductase (InterPro:IPR001709), Ferredoxin--NADP reductase (InterPro:IPR012146); BEST Arabidopsis thaliana protein match is: ferredoxin-NADP(+)-oxidoreductase 2 (TAIR:AT1G20020.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT5G66190.2

Model type
Protein coding
Short Description
ferredoxin-NADP(+)-oxidoreductase 1
Curator Summary
Encodes a leaf-type ferredoxin:NADP(H) oxidoreductase. It is present in both chloroplast stroma and thylakoid membranes but is more abundant in the thylakoid. The affinity of this enzyme for ferredoxin is slightly, but significantly, higher than AtLFNR2, an isoform of the same enzyme. AtLFNR1 forms a heterodimer with AtFNR2 and is also a prerequisite to attach AtFNR2 to the thylakoid membrane.
Computational Description
LEAF FNR 1 (ATLFNR1); FUNCTIONS IN: oxidoreductase activity, electron transporter, transferring electrons within the cyclic electron transport pathway of photosynthesis activity, poly(U) RNA binding, NADPH dehydrogenase activity, electron transporter, transferring electrons within the noncyclic electron transport pathway of photosynthesis activity; INVOLVED IN: oxidation reduction, photosynthetic electron transport chain, defense response to bacterium; LOCATED IN: thylakoid, chloroplast thylakoid membrane, apoplast, chloroplast stroma, chloroplast; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Ferredoxin reductase-type FAD-binding domain (InterPro:IPR017927), Oxidoreductase, FAD-binding domain (InterPro:IPR008333), Ferredoxin--NADP reductase (InterPro:IPR012146), Oxidoreductase FAD/NAD(P)-binding (InterPro:IPR001433), Riboflavin synthase-like beta-barrel (InterPro:IPR017938), Ferredoxin Reductase (InterPro:IPR015701), Flavoprotein pyridine nucleotide cytochrome reductase (InterPro:IPR001709); BEST Arabidopsis thaliana protein match is: ferredoxin-NADP(+)-oxidoreductase 2 (TAIR:AT1G20020.3); Has 7407 Blast hits to 7407 proteins in 1806 species: Archae - 24; Bacteria - 3962; Metazoa - 817; Fungi - 811; Plants - 610; Viruses - 0; Other Eukaryotes - 1183 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT5G66200.1

Model type
Protein coding
Short Description
armadillo repeat only 2
Curator Summary
Armadillo repeat protein. One of a family of four in Arabidopsis. Expressed in vegetative tissues, anthers and ovules.
Computational Description
armadillo repeat only 2 (ARO2); FUNCTIONS IN: binding; INVOLVED IN: biological_process unknown; LOCATED IN: plasma membrane; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Armadillo-like helical (InterPro:IPR011989), Armadillo (InterPro:IPR000225), Armadillo-type fold (InterPro:IPR016024); BEST Arabidopsis thaliana protein match is: armadillo repeat only 1 (TAIR:AT4G34940.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).
Link
InterPro Scan - TAIR