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Mutant Lines
54-4853-1

54-4853-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

This line has NOT been observed.

Determination of insertion point(s)

5 prime edge [+] show sequence
1. Chromosome: 5; Position: 26456399 [Map]; Strand: c
  - AT5G66200.1 - [+] show detail - armadillo repeat only 2
    Distance: -577; Region: promoter
  - AT5G66210.4 - [+] show detail - calcium-dependent protein kinase 28
    Distance: 0; Region: exon11 (3UTR)
  - AT5G66210.2 - [+] show detail - calcium-dependent protein kinase 28
    Distance: 0; Region: exon14 (3UTR)
  - AT5G66210.1 - [+] show detail - calcium-dependent protein kinase 28
    Distance: 0; Region: exon12 (3UTR)
  - AT5G66210.3 - [+] show detail - calcium-dependent protein kinase 28
    Distance: 0; Region: exon12 (3UTR)
Flanking Genome Sequence: 54-4853-1 - 5-prime edge
```
CTCTTAAGGT GCACTAGATG CAAGAGGACA CGTGGCATTC CCTGAATGGG 
GGACAAATCC AATTTTAAAT TTTTAATGGT TGGGCACCAA AAGAAACATA 
GAGAGCCAGC CTGTAATTTC GATTCTATCA TATATTTTGT CTGTTTCCAT 
TTTTCTTATA ATATTCGTTT TGAGAGCACC AAACAAACCC TTTTCTATGA 
GTATTTTCCA GCCAGGATCT GAGTGGCTCC AACGAATTTA GTAAACAATG 
ATTGATTAAA TGTTTATTAA GGGAATATCT CAAATCTTAT TGGATATAAA 
AAATAATAAA ATCATCGCAA GTGACAGTGG TCTATAAATG TGTGCATTTT 
CGCAGGTTCG GTGAATGTAA ATGGGTGAGT CTCTGAATTT GACTAGNCAC 
GTTNAAAGAT ACTAATAATN TTGGANTATG ATAACTNGGN NTNNNNNNAN 
AAANGNNGAN GAAAAGANAC TNNANNNGGG GGGNTNNNNA AANAAAANNC 
NNNNNGGNNG NNTTNNNNNN NNNCC
```

3 prime edge [+] show sequence

Chromosome: 5; Position: 26456515 [Map]; Strand: c
- AT5G66200.1 - [+] show detail - armadillo repeat only 2
  Distance: -693; Region: promoter
- AT5G66210.4 - [+] show detail - calcium-dependent protein kinase 28
  Distance: 0; Region: exon11 (3UTR)
- AT5G66210.2 - [+] show detail - calcium-dependent protein kinase 28
  Distance: 0; Region: exon14 (3UTR)
- AT5G66210.1 - [+] show detail - calcium-dependent protein kinase 28
  Distance: 0; Region: exon12 (3UTR)
- AT5G66210.3 - [+] show detail - calcium-dependent protein kinase 28
  Distance: 0; Region: exon12 (CDS)

CTTAAGAGCT CTCTCATATT TCATCAATGG GATTCTCACT CCACTTTCTC 
TCTTTGTTTT GCTGCAGCCT TAAACAACCA AAGTATTACT TACTTATCAC 
TACAAACGCC AAAAACCCCA CTTATCTTAC AACCAACATT ACATATAAGA 
AGATAAGACA TATCACATCC CAACAAATTA CATTCTGTAT CCTTTACTTA 
CTCCTCTTCT AGTATATACT ATCGAAGATT CCTGTGACCT GCAGGGCTTG 
GTGCTCTCTG TGAACTTATG CTCGCTGTTC TTAGAAGTCT CCTGAACTCA 
TGCAGGCTTA TTTTCCCATC TCTGTCTATG TCTGCTTCAT CCAGCAGTGG 
ATCTATTGAT CCTCTTAACC CCGTGTGCTG AGGATTCACA AACAAATTGG 
GTTTCATCAC CCACTTACCT AAACATCTAC TCTACCGTAC GAGAATATTG 
ATAAGAGGTT AGTTCAGCTT ACCATTCGAA GTTCCTCAGG CGTTATGTAC 
CCGTCTTTGT CTAGGTCGAA TTTCTCAAAA GCTGCTCTTG ACCTTAGCTG 
CCATTTCTCT GAATCATGTT CTTCTAGTTG ATGAACATGT AGAGCTGCTG 
CTACAAACTC TGTGAAGTCC ACTAACCCAT CAGTGTTGCT ATCAATCTGT 
GACCAGCAAA GATACAAGTA CACAACGGCT CATTATGAAA CTACGGTCTC 
CCTCTGATAC CCCCAAAGAT GCAATTGGCA ATGGGAAACA AAAATATNAC 
TA

AT5G66200.1

Model type: Protein coding
Short Description: armadillo repeat only 2
Curator Summary: Armadillo repeat protein. One of a family of four in Arabidopsis. Expressed in vegetative tissues, anthers and ovules.
Computational Description: armadillo repeat only 2 (ARO2); FUNCTIONS IN: binding; INVOLVED IN: biological_process unknown; LOCATED IN: plasma membrane; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Armadillo-like helical (InterPro:IPR011989), Armadillo (InterPro:IPR000225), Armadillo-type fold (InterPro:IPR016024); BEST Arabidopsis thaliana protein match is: armadillo repeat only 1 (TAIR:AT4G34940.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).
Link: InterPro Scan - TAIR

AT5G66210.1

Model type: Protein coding
Short Description: calcium-dependent protein kinase 28
Curator Summary: member of Calcium Dependent Protein Kinase
Computational Description: calcium-dependent protein kinase 28 (CPK28); FUNCTIONS IN: protein serine/threonine kinase activity, calmodulin-dependent protein kinase activity, protein kinase activity, calcium ion binding, ATP binding; INVOLVED IN: protein amino acid phosphorylation; LOCATED IN: plasma membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Protein kinase, ATP binding site (InterPro:IPR017441), EF-Hand 1, calcium-binding site (InterPro:IPR018247), Serine/threonine-protein kinase domain (InterPro:IPR002290), Calcium-binding EF-hand (InterPro:IPR002048), EF-hand-like domain (InterPro:IPR011992), Serine/threonine-protein kinase-like domain (InterPro:IPR017442), Serine/threonine-protein kinase, active site (InterPro:IPR008271), Protein kinase-like domain (InterPro:IPR011009), Protein kinase, catalytic domain (InterPro:IPR000719), EF-HAND 2 (InterPro:IPR018249), Calcium-dependent protein kinase (InterPro:IPR020642), Tyrosine-protein kinase, catalytic domain (InterPro:IPR020635), Calcium/calmodulin-dependent protein kinase-like (InterPro:IPR020636); BEST Arabidopsis thaliana protein match is: calcium-dependent protein kinase 16 (TAIR:AT2G17890.1); Has 124563 Blast hits to 121753 proteins in 3731 species: Archae - 157; Bacteria - 14240; Metazoa - 45960; Fungi - 14836; Plants - 27830; Viruses - 455; Other Eukaryotes - 21085 (source: NCBI BLink).
Link: InterPro Scan - TAIR

AT5G66210.2

Model type: Protein coding
Short Description: calcium-dependent protein kinase 28
Curator Summary: member of Calcium Dependent Protein Kinase
Computational Description: calcium-dependent protein kinase 28 (CPK28); FUNCTIONS IN: protein serine/threonine kinase activity, calmodulin-dependent protein kinase activity, protein kinase activity, ATP binding, calcium ion binding; INVOLVED IN: protein amino acid phosphorylation; LOCATED IN: plasma membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Protein kinase, ATP binding site (InterPro:IPR017441), EF-Hand 1, calcium-binding site (InterPro:IPR018247), Serine/threonine-protein kinase domain (InterPro:IPR002290), EF-hand-like domain (InterPro:IPR011992), Calcium-binding EF-hand (InterPro:IPR002048), Serine/threonine-protein kinase-like domain (InterPro:IPR017442), Serine/threonine-protein kinase, active site (InterPro:IPR008271), Protein kinase-like domain (InterPro:IPR011009), Protein kinase, catalytic domain (InterPro:IPR000719), EF-HAND 2 (InterPro:IPR018249), Calcium-dependent protein kinase (InterPro:IPR020642), Calcium/calmodulin-dependent protein kinase-like (InterPro:IPR020636), Tyrosine-protein kinase, catalytic domain (InterPro:IPR020635); BEST Arabidopsis thaliana protein match is: calcium-dependent protein kinase 16 (TAIR:AT2G17890.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).
Link: InterPro Scan - TAIR

AT5G66210.3

Model type: Protein coding
Short Description: calcium-dependent protein kinase 28
Curator Summary: member of Calcium Dependent Protein Kinase
Computational Description: calcium-dependent protein kinase 28 (CPK28); FUNCTIONS IN: protein serine/threonine kinase activity, calmodulin-dependent protein kinase activity, protein kinase activity, ATP binding, calcium ion binding; INVOLVED IN: protein amino acid phosphorylation; LOCATED IN: plasma membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Protein kinase, ATP binding site (InterPro:IPR017441), EF-Hand 1, calcium-binding site (InterPro:IPR018247), Serine/threonine-protein kinase domain (InterPro:IPR002290), Calcium-binding EF-hand (InterPro:IPR002048), EF-hand-like domain (InterPro:IPR011992), EF-hand (InterPro:IPR018248), Serine/threonine-protein kinase-like domain (InterPro:IPR017442), Serine/threonine-protein kinase, active site (InterPro:IPR008271), Protein kinase-like domain (InterPro:IPR011009), Protein kinase, catalytic domain (InterPro:IPR000719), EF-HAND 2 (InterPro:IPR018249), Calcium-dependent protein kinase (InterPro:IPR020642), Tyrosine-protein kinase, catalytic domain (InterPro:IPR020635), Calcium/calmodulin-dependent protein kinase-like (InterPro:IPR020636); BEST Arabidopsis thaliana protein match is: calcium-dependent protein kinase 16 (TAIR:AT2G17890.1); Has 122907 Blast hits to 120110 proteins in 3700 species: Archae - 157; Bacteria - 14345; Metazoa - 44968; Fungi - 14295; Plants - 27583; Viruses - 455; Other Eukaryotes - 21104 (source: NCBI BLink).
Link: InterPro Scan - TAIR

AT5G66210.4

Model type: Protein coding
Short Description: calcium-dependent protein kinase 28
Curator Summary: member of Calcium Dependent Protein Kinase
Computational Description: calcium-dependent protein kinase 28 (CPK28); FUNCTIONS IN: protein serine/threonine kinase activity, calmodulin-dependent protein kinase activity, protein kinase activity, calcium ion binding, ATP binding; INVOLVED IN: protein amino acid phosphorylation; LOCATED IN: plasma membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Protein kinase, ATP binding site (InterPro:IPR017441), EF-Hand 1, calcium-binding site (InterPro:IPR018247), Serine/threonine-protein kinase domain (InterPro:IPR002290), EF-hand-like domain (InterPro:IPR011992), Serine/threonine-protein kinase-like domain (InterPro:IPR017442), Protein kinase-like domain (InterPro:IPR011009), Serine/threonine-protein kinase, active site (InterPro:IPR008271), Protein kinase, catalytic domain (InterPro:IPR000719), EF-HAND 2 (InterPro:IPR018249), Calcium-dependent protein kinase (InterPro:IPR020642), Calcium/calmodulin-dependent protein kinase-like (InterPro:IPR020636); BEST Arabidopsis thaliana protein match is: calcium-dependent protein kinase 16 (TAIR:AT2G17890.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
Link: InterPro Scan - TAIR