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54-5017-3

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

This line has NOT been observed.

Determination of insertion point(s)

  • 5 prime edge [+] show sequence

    1. Chromosome: 1; Position: 8123298 [Map]; Strand: c
      • AT1G22940.1 - [+] show detail - thiamin biosynthesis protein, putative
        Distance: 0; Region: intron4 (CDS)
    Flanking Genome Sequence: 54-5017-3 - 5-prime edge 
    CCTAAAAGGA TTAACTTTAT GTCCAGGGCA CCACAAGCAG AAAGGTTTCT 
    TCTCAATTCG TCCCAAGTTG TAGGTTAAAT TAAGGGATTA CTAAAAAAAG 
    TTGTAGACTA TATAGATTAG AGAGTAACGG CAGAAAATAG AAACCTAGGA 
    CCCATTTCAT GCAACGACTT TGCTGCAGAC CGCATTTCTG CAACAGTCTC 
    AATCCGAAAA CCATCAAGTA AAGCAGAAGC CTCTTTCACA TTTGGGGTAA 
    TTATGTCAGC AATTGGTAGT AATCTCTCTC TGCAATAAGA AACCATAGTT 
    AATTAACAAC ATAACACACC AAATCACTGA AGTCAAACAC ATAATGACAA 
    CAAATGCATA AAAGCT

  • 3 prime edge [+] show sequence

    1. Chromosome: 1; Position: 8123414 [Map]; Strand: c
      • AT1G22940.1 - [+] show detail - thiamin biosynthesis protein, putative
        Distance: 0; Region: intron4 (CDS)
    Flanking Genome Sequence: 54-5017-3 - 3-prime edge 
    CTTTTAGGCT TGATTATTAT GTGGTTATGC TCAGTGTATG AGCATTATTG 
    TGTGATGTTG ATCCACTTTT TTTTGTGCTT CAGATTCGTA CTTGTTAAAG 
    GTGGTGATCT TCCTGACTCA TCANATTCAG TAGATGTTTA CNTTTGATGG 
    TATGATGATA GCACAGNGAT CTTTTTATTC TAATTTTGTC TTTGTTGTTG 
    AGGAGGCTTA ACGGACTCAA TACATTTGTG GTTATAAAAA TTAGGAACTN 
    GTTTAGGCAA GGAGTTTCAT GAACTCCGTT CTCCTCGCAT AGCTACAAGA 
    AATACTCATG GGACTGGNTG CACTTTGGCT TNCAGAATNG CAGCTGANCT 
    TGCAAAAGGN TCTTNAATGN GNTNAANCGA NNNGGTTTNN NNTATANCTN 
    NGCTTATTTT TNAAAAACAT ACNCNTNNCC NACCCGTGNT ANGNNANNNN 
    GGNCTNTGNA NGGNGTTAAG ATAAAAAAAC NNGNNCCCGN CGCNTNNCNC 
    CNNANAAACN TNTCTNGAGN NANTTTNGNT GNGCGNGGNA NCCNCTATNN 
    GCATCNNAAG NTNCTGGGAA NNGNATNTNN CTCGAAANGG GGGTNCTGGG 
    GGGNAAANNA ACCCCNNCCN NNCAATAAAA NCAAAGNTNA NCATAAATTN 
    GNTGGGGAGN AAAANNNGGG AAAAAGNGNG AGGGGGGGGG GANAAAAAGC 
    GGGNNCCGCN TTTAAAACCN NCGTCNNATT NAGGGCCGCN CCC

AT1G22940.1

Model type
Protein coding
Short Description
thiamin biosynthesis protein, putative
Curator Summary
Encodes a bifunctional enzyme required for thiamine (vitamin B1) biosynthesis. TH1 can phosphorylate HMP-P to produce HMP-PP, the pyrimidine heterocyclic subunit of thiamine. TH1 also catalyzes the condensation of HMP-PP and HET to form thiamine monophosphate (TMP). TH1 also appears capable of phosphorylating HMP based on E.coli mutant complementation assays. th1 mutants are thiamine auxotrophs that die as seedlings on unsupplemented media.
Computational Description
THIAMINE REQUIRING 1 (TH1); FUNCTIONS IN: phosphomethylpyrimidine kinase activity, thiamin-phosphate diphosphorylase activity, hydroxymethylpyrimidine kinase activity; INVOLVED IN: thiamin biosynthetic process; LOCATED IN: chloroplast; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Thiamine monophosphate synthase (InterPro:IPR003733), Phosphomethylpyrimidine kinase type-2 (InterPro:IPR004399), Aldolase-type TIM barrel (InterPro:IPR013785), Phosphomethylpyrimidine kinase type-1 (InterPro:IPR013749); Has 15989 Blast hits to 15891 proteins in 2548 species: Archae - 338; Bacteria - 12149; Metazoa - 165; Fungi - 331; Plants - 107; Viruses - 0; Other Eukaryotes - 2899 (source: NCBI BLink).
Link
InterPro Scan - TAIR