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ET12623

CSHL Arabidopsis genetrap mutant lines

Original: ET12623

Phenotypes

Morphological phenotypes

Specific phenotypes were NOT observed.

Original: ET12623

Determination of insertion point(s)

  • 3 prime edge [+] show sequence

    1. Chromosome: 3; Position: 23452582 [Map]; Strand: w
      • AT3G63510.1 - [+] show detail - FMN-linked oxidoreductases superfamily protein
        Distance: 0; Region: exon12 (CDS)
      • AT3G63510.2 - [+] show detail - FMN-linked oxidoreductases superfamily protein
        Distance: 0; Region: exon12 (CDS)
      • AT3G63520.1 - [+] show detail - carotenoid cleavage dioxygenase 1
        Distance: -329; Region: promoter
    Flanking Genome Sequence: ET12623 - 3-prime edge 
    TACATTGTGC ACATCTGCGA ACACATCTTC ATCTCCGCTC ACAGGACTCC 
    CAGATATGGG TGAATCTAAG ACACAGTCCG GGATCGCCCT CAAGCTTTCT 
    TCCAGCAACG ATCCAACACT CTGCAATACA TAATTCAACC ACTCAACACC 
    AGTTACAAAC TTTCTAACGT GTTACCACCA ATATATCACA CACCCTGCAC 
    TCCTTGAAAG CTGCGTCTGC TCTCCGCTTC CACAAGCTAT TTCCATTTTC 
    TGAATGGAAA AGGTTTAGCA GAGGCTGCAA TCAATTGAAA CCATCTTCAT 
    CTCACTCGA

  • 5 prime edge [+] show sequence

    1. Chromosome: 3; Position: 23452720 [Map]; Strand: w
      • AT3G63510.1 - [+] show detail - FMN-linked oxidoreductases superfamily protein
        Distance: 0; Region: exon12 (3UTR)
      • AT3G63510.2 - [+] show detail - FMN-linked oxidoreductases superfamily protein
        Distance: 0; Region: exon12 (3UTR)
      • AT3G63520.1 - [+] show detail - carotenoid cleavage dioxygenase 1
        Distance: -191; Region: promoter
    Flanking Genome Sequence: ET12623 - 5-prime edge 
    CGAAGCTGGA GAAGAAATCA TCTTATGCGC TTAGTTGTCG TTTGTAATCT 
    GTGTTTGTGA TTGTCTCTGT CGATTAAAAT TGTAAATTGA ACGGGGCTTA 
    CTTCAAGTGG TTGTTAGTCT TGGGCTCGTG TATGGGCTTT TTTGTTATTC 
    TTTTTGTTTG GGCCGGAAAT ATAACTGGGT CCATCATCTC AAGAAAAAAG 
    ATTCCGTTGC GACGTGGCAG CGTTTCCGCC AAGACGCGGG CCATATTATC 
    TGGTCTCAAA TCACCTCCTC CTTGCAGCAA AACAAACAAT CATGGCGGAG 
    AAACTCAGTG ATGGCAGCAT CATCATCTCA GTCCATCCTA GACCCTCCAA 
    GGGTTTCTCC TCGAAGCTTC TCGATCTTCT CGAGAGACTT GTTGTCAAGC 
    TCATGCACGA TGCTTCTCTC CCTCTCCACT ACCTCTCAGG CAACTTCGCT 
    CCCATCCGTG ATGAAACTCC TCCCGTCAAG GATCTCCCCG TCCATGGATT 
    TCTTCCCGTG AAATTCTCTC ATATTCTCTT TGATCCTTTC CCCTAAGGGT 
    TATTTAGTAT TCTTATGATC GTCTCTTTTG TCTCGCAGGA ATGCTTGAAT 
    GGTGAATTTG TGAGGGTTGG TCCAAACCCC AAGTTTGATG CTGTCGCTGG 
    ATATCACTGG CACGTT

AT3G63510.1

Model type
Protein coding
Short Description
FMN-linked oxidoreductases superfamily protein
Computational Description
FMN-linked oxidoreductases superfamily protein; FUNCTIONS IN: tRNA dihydrouridine synthase activity, FAD binding, catalytic activity; INVOLVED IN: regulation of nitrogen utilization, tRNA processing, oxidation reduction, metabolic process; EXPRESSED IN: 18 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Aldolase-type TIM barrel (InterPro:IPR013785), tRNA-dihydrouridine synthase (InterPro:IPR001269); BEST Arabidopsis thaliana protein match is: Aldolase-type TIM barrel family protein (TAIR:AT5G47970.2); Has 11920 Blast hits to 11916 proteins in 2583 species: Archae - 58; Bacteria - 8071; Metazoa - 278; Fungi - 218; Plants - 160; Viruses - 0; Other Eukaryotes - 3135 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT3G63510.2

Model type
Protein coding
Short Description
FMN-linked oxidoreductases superfamily protein
Computational Description
FMN-linked oxidoreductases superfamily protein; FUNCTIONS IN: tRNA dihydrouridine synthase activity, FAD binding, catalytic activity; INVOLVED IN: regulation of nitrogen utilization, tRNA processing, oxidation reduction, metabolic process; EXPRESSED IN: 18 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Aldolase-type TIM barrel (InterPro:IPR013785), tRNA-dihydrouridine synthase (InterPro:IPR001269); BEST Arabidopsis thaliana protein match is: Aldolase-type TIM barrel family protein (TAIR:AT5G47970.2); Has 11664 Blast hits to 11660 proteins in 2553 species: Archae - 58; Bacteria - 8007; Metazoa - 234; Fungi - 152; Plants - 146; Viruses - 0; Other Eukaryotes - 3067 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT3G63520.1

Model type
Protein coding
Short Description
carotenoid cleavage dioxygenase 1
Curator Summary
Encodes a protein with 9-cis-epoxycarotenoid dioxygenase activity. The enzyme was shown to act on a variety of carotenoid including β-carotene, lutein, zeaxanthin, and all-trans-violaxanthin. When those compounds are used as substrates, the major reaction product detected is a C14 dialdehyde: 4,9-dimethyldodeca-2,4,6,8,10-pentaene-1,12-dial. The enzyme did not cleave as efficiently carotenoids containing 9-cis-double or allenic bonds.
Computational Description
carotenoid cleavage dioxygenase 1 (CCD1); FUNCTIONS IN: 9-cis-epoxycarotenoid dioxygenase activity; INVOLVED IN: response to water deprivation, carotene catabolic process, xanthophyll catabolic process, carotenoid catabolic process; LOCATED IN: plasma membrane, vacuole, cytoplasm; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Carotenoid oxygenase (InterPro:IPR004294); BEST Arabidopsis thaliana protein match is: nine-cis-epoxycarotenoid dioxygenase 4 (TAIR:AT4G19170.1); Has 2926 Blast hits to 2896 proteins in 500 species: Archae - 16; Bacteria - 808; Metazoa - 317; Fungi - 194; Plants - 866; Viruses - 0; Other Eukaryotes - 725 (source: NCBI BLink).
Link
InterPro Scan - TAIR