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ET13364

CSHL Arabidopsis genetrap mutant lines

Original: ET13364

Phenotypes

Morphological phenotypes

Specific phenotypes were NOT observed.

Original: ET13364

Determination of insertion point(s)

Notice

More than one insertion position or no insertion point is expected.

  • 3 prime edge [+] show sequence

    1. Chromosome: 1; Position: 26250435 [Map]; Strand: c
      • AT1G69770.1 - [+] show detail - chromomethylase 3
        Distance: 0; Region: exon12 (CDS)
    Flanking Genome Sequence: ET13364 - 3-prime edge
    GGTAGAGGTA TTTTACCGAC CGTTACCGAC CGTTTTCATT TCATATACAC 
    CAAAAGCTGC TTGTTTTTCT GATCTTCTAG AGGGTCCAAT AAGTTCCTGA
    AGCGGTTGTG ACCACTGATT CCTTGGCATG GTGGCCCACC GCAGACAACA
    TCAACACCTC CCTAAACATC AACAACATAT AAACAATGTC AGGATATTGC
    TCTAAACATC AACTGTTCTG TGACTAAATC TATCACCCCT CAACAATTAA
    CTGACGCAGA ACAACATAAG ACCCATAGAA CTTATCACAT ACAATAACTT
    AGATTATTGG CTTTTAGATT AAATATCTGA AAATTATGCA CTAGCTTCAG
    AAGTTTAAGG AAAGCAAAAG AATCAGGTAT AAAAAATACA TACTGGTAAC
    GGAAGGATGC CAGATTTATA TCCAAGTTTA ACGAACTCTC CAATTTTACC
    CCGGCAATTA CTGTTCAAGG ACAAATTTAG AGATTTTAAC AACGCATTAA
    CATATATAAC TTTAATGCAA ACATTCTTAA TGTTTTGCTC ACCTGAGTCC
    TTCAATAGGC TCCCATGTAT CATGAGAATC ATCATAATTC AGCCACCTTA
    CCTATTATAT CAAAACACCA GAGATGATTA ACTATATAAC AAGGCTAAAC
    CATGAGATGT AATGTGAAAT TTGTACAAGC AGAGCGACAA TACCTTCAAA
    TAAAGTCCAC GTTTCAATAA CTTTTTAGGG A
  • 5 prime edge [+] show sequence

    1. Chromosome: 1; Position: 26394668 [Map]; Strand: w
      • AT1G70070.1 - [+] show detail - DEAD/DEAH box helicase, putative
        Distance: -472; Region: promoter
    Flanking Genome Sequence: ET13364 - 5-prime edge
    CTTTATCCCT GGTTGCGGCT GCGGATACAA ATTTGGCTTG GCCATTCACG 
    TGATCCCAAA CTCTGAAGAC TCTTAAAGAG TGATGAGATC GACATACAAA
    GAGGTGATAT AATCTTTGAT CTTCTCTCCA GCGAAGGTGT ACCCATCTCC
    ATACTTATAG CACACATCTG CTAAGCGTCC GTAATTCAAA ACAGGCCTTA
    GAATATGAAG CGGTATAAAT TTGGCTGCCT TCAAGAACTC TTCGTTCATG
    ACCTTAGACA TGTGATTAGT CTTTTTCTCA AACTCAATAC ACGCTTCTTC
    CTCACTTACT TTGTGTTGCT TCGTATAACA GTTGATCCCA TTACAAACAT
    CTCCTCT

AT1G69770.1

Model type
Protein coding
Short Description
chromomethylase 3
Curator Summary
Encodes a chromomethylase involved in methylating cytosine residues at non-CG sites. Involved in preferentially methylating transposon-related sequences, reducing their mobility. CMT3 interacts with an Arabidopsis homologue of HP1 (heterochromatin protein 1), which in turn interacts with methylated histones. Involved in gene silencing.
Computational Description
chromomethylase 3 (CMT3); FUNCTIONS IN: DNA (cytosine-5-)-methyltransferase activity; INVOLVED IN: in 6 processes; LOCATED IN: chromatin, nucleus; EXPRESSED IN: 21 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: DNA methylase, C-5 cytosine-specific (InterPro:IPR001525), Chromo domain-like (InterPro:IPR016197), Bromo adjacent homology (BAH) domain (InterPro:IPR001025), DNA methylase, C-5 cytosine-specific, active site (InterPro:IPR018117), Chromo domain (InterPro:IPR000953); BEST Arabidopsis thaliana protein match is: chromomethylase 2 (TAIR:AT4G19020.1); Has 4337 Blast hits to 3739 proteins in 974 species: Archae - 208; Bacteria - 2545; Metazoa - 230; Fungi - 161; Plants - 416; Viruses - 30; Other Eukaryotes - 747 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT1G70070.1

Model type
Protein coding
Short Description
DEAD/DEAH box helicase, putative
Curator Summary
Allelic to ISE2(increased size exclusion limit of plasmodesmata 2). Mutants maintain dilated plasmodesmata at the embryonic torpedo stage.
Computational Description
EMBRYO DEFECTIVE 25 (EMB25); FUNCTIONS IN: RNA helicase activity, ATP-dependent helicase activity; INVOLVED IN: posttranscriptional gene silencing, plasmodesmata-mediated intercellular transport, embryo development ending in seed dormancy; LOCATED IN: stress granule, chloroplast stroma, chloroplast; EXPRESSED IN: 21 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: DNA/RNA helicase, DEAD/DEAH box type, N-terminal (InterPro:IPR011545), DSH, C-terminal (InterPro:IPR012961), DEAD-like helicase, N-terminal (InterPro:IPR014001), DNA/RNA helicase, C-terminal (InterPro:IPR001650), Helicase, superfamily 1/2, ATP-binding domain (InterPro:IPR014021); BEST Arabidopsis thaliana protein match is: RNA helicase, ATP-dependent, SK12/DOB1 protein (TAIR:AT3G46960.1); Has 21396 Blast hits to 12786 proteins in 1473 species: Archae - 1027; Bacteria - 4600; Metazoa - 5457; Fungi - 2724; Plants - 1158; Viruses - 307; Other Eukaryotes - 6123 (source: NCBI BLink).
Link
InterPro Scan - TAIR