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ET13392

CSHL Arabidopsis genetrap mutant lines

Original: ET13392

Phenotypes

Morphological phenotypes

Specific phenotypes were NOT observed.

Original: ET13392

Determination of insertion point(s)

  • 3 prime edge [+] show sequence

    1. Chromosome: 5; Position: 460277 [Map]; Strand: w
      • AT5G02250.1 - [+] show detail - Ribonuclease II/R family protein
        Distance: 750; Region: intergenic
      • AT5G02260.1 - [+] show detail - expansin A9
        Distance: -2881; Region: intergenic
    Flanking Genome Sequence: ET13392 - 3-prime edge
    ATGAAACGGT AGAGGTATTT TACCGACCGT TACCGACCGT TTTCATCCCT 
    ACTAGTCGGT GACTTGTATT AATGGTTAGA TAACATAGTA ACATGAAAAT
    ATTCTTTTAA TTGTTGTTGC TTCACATTAA TTTTTTTATG ATACGCTTAT
    CTACTACGAC GTACACTTTC AGCATTTGAC TTTTTCTTTC TTCCCACCAC
    TCGATACGAT TTTGACTATT TTAAATAACG TACGAATTGT ATTTCTTTGT
    CATAATCCCA CCCAACTTAT CATTAGCTTA CAAGTAAATA TTTTCTTGTG
    TTGGAAATTG AATTAGATAA TAGGCGTGGA GAAAGAGAAG CTACCATTTC
    ATCTGGTTTC AGTTTTAATC ATTAGCATAT ATAACTGTAC AACCGACTAA
    TCGAGATATT CGATAGTATT TGCAAGTTTC TGATTTATCA ATAAGTAATT
    TAACGCAATT TGTTATTGGT AAAAATGTCA AATGATTTTA GATTTGGACA
    GCCATGTGGT GTTATGGGGT TTACGGGCAC TTAATCTGTC TTTAAAAAAA
    AAAATCGACG ATCAACCTTA TTTATAATAA CAAAGACCTT TTTAAATAAG
    AAAAGACAAA AATCGAAACC AAAAACTAA

AT5G02250.1

Model type
Protein coding
Short Description
Ribonuclease II/R family protein
Curator Summary
Encodes a exoribonuclease involved in rRNA processing in mitochondria and chloroplasts.Loss of function mutations are pale green and require supplementation with sucrose for germination and early development. Plants are pale green due to defects in chloroplast biogenesis.
Computational Description
EMBRYO DEFECTIVE 2730 (EMB2730); FUNCTIONS IN: ribonuclease activity, 3'-5'-exoribonuclease activity, RNA binding; INVOLVED IN: chloroplast organization, rRNA processing, embryo development ending in seed dormancy; LOCATED IN: mitochondrion, chloroplast; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Ribonuclease II/R (InterPro:IPR001900); BEST Arabidopsis thaliana protein match is: ribonuclease II family protein (TAIR:AT2G17510.1); Has 7586 Blast hits to 7556 proteins in 2301 species: Archae - 41; Bacteria - 5322; Metazoa - 356; Fungi - 403; Plants - 125; Viruses - 2; Other Eukaryotes - 1337 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT5G02260.1

Model type
Protein coding
Short Description
expansin A9
Curator Summary
member of Alpha-Expansin Gene Family. Naming convention from the Expansin Working Group (Kende et al, 2004. Plant Mol Bio)
Computational Description
expansin A9 (EXPA9); INVOLVED IN: plant-type cell wall modification involved in multidimensional cell growth, unidimensional cell growth, plant-type cell wall loosening; LOCATED IN: chloroplast; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 11 growth stages; CONTAINS InterPro DOMAIN/s: Barwin-related endoglucanase (InterPro:IPR009009), Pollen allergen, N-terminal (InterPro:IPR014734), Expansin (InterPro:IPR002963), Rare lipoprotein A (InterPro:IPR005132), Expansin/Lol pI (InterPro:IPR007118), Expansin 45, endoglucanase-like (InterPro:IPR007112), Pollen allergen/expansin, C-terminal (InterPro:IPR007117); BEST Arabidopsis thaliana protein match is: Barwin-like endoglucanases superfamily protein (TAIR:AT2G37640.1); Has 2170 Blast hits to 2166 proteins in 155 species: Archae - 0; Bacteria - 2; Metazoa - 0; Fungi - 38; Plants - 2091; Viruses - 0; Other Eukaryotes - 39 (source: NCBI BLink).
Link
InterPro Scan - TAIR