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ET13541

CSHL Arabidopsis genetrap mutant lines

Original: ET13541

Phenotypes

Morphological phenotypes

Specific phenotypes were NOT observed.

Original: ET13541

Determination of insertion point(s)

  • 3 prime edge [+] show sequence

    1. Chromosome: 1; Position: 8181310 [Map]; Strand: w
      • AT1G23080.1 - [+] show detail - Auxin efflux carrier family protein
        Distance: 0; Region: intron3 (CDS)
      • AT1G23080.3 - [+] show detail - Auxin efflux carrier family protein
        Distance: 0; Region: intron3 (CDS)
      • AT1G23080.2 - [+] show detail - Auxin efflux carrier family protein
        Distance: 0; Region: exon3 (3UTR)
    Flanking Genome Sequence: ET13541 - 3-prime edge
    TTTTACCGAC CGTAGATTCA TCATCATTTA TTGGATTTAG ATTACGTTTA 
    CATGTATTGC ATGATTCAGC ACTTGTTGTT TTGAGTGGAA ATGAATTAAC
    TGAAAATGTG AAAATGGTGA AGGGTTGTTC ATGGCATTGC AACCGAAATT
    AATTGCTTGC GGAAATTCGA CGGCGACTTT TGCGATGGCG GTGAGATTCT
    TTACTGGACC AGCGGTAATG GCCGTGGCAG CAATGGCTAT TGGATTACGT
    GGAGACCTAT TGCGTGTGGC CATTGTTCAA GTAAATTAAA CTATTTTCGT
    TTGTTGTTAA CAATCTCTTT GTAAGAAGTA GTTTAACCAA ATCTTGTGGT
    AACGTTGTAG GCTGCATTGC CTCAAGGGAT CGTGCCGTTT GTGTTTGCAA
    AAGAGTATAA TGTTCATCCC GCAATCTTGA GTACAGGGTA AGTATTTCAT
    ATTGAACCAA TAGTAAAACT TTTCTTATGC ATAAGAGAAG ATTACTAATA
    TTGTTGATTC TTATGTGTAT TATTGCAGGG TAATATTTGG AATGCTTATC
    GCACTACCGA TTACACTTGT TTACTACATT TTACTCGGGC TATAAGCTCT
    TCAATGGAGA AGTTCTGCTT GGCTGANGAG GATAAAGAGA AACAAGATAA
    TTCCAAGATT AGTGGAACGC AACTAGAAAA TGTAATGTAA CACGAAATGT
    AGTAAATTTA AAGGGACCAA GATTTGATTT GAGGATCCAA AAACCCTTT

AT1G23080.1

Model type
Protein coding
Short Description
Auxin efflux carrier family protein
Curator Summary
Encodes a novel component of auxin efflux that is located apically in the basal cell and is involved during embryogenesis in setting up the apical-basal axis in the embryo. It is also involved in pattern specification during root development. In roots, it is expressed at lateral and basal membranes of provascular cells in the meristem and elongation zone, whereas in the columella cells it coincides with the PIN3 domain. Plasma membrane-localized PIN proteins mediate a saturable efflux of auxin. PINs mediate auxin efflux from mammalian and yeast cells without needing additional plant-specific factors. The action of PINs in auxin efflux is distinct from PGPs, rate-limiting, specific to auxins and sensitive to auxin transport inhibitors. PINs are directly involved of in catalyzing cellular auxin efflux.
Computational Description
PIN-FORMED 7 (PIN7); FUNCTIONS IN: auxin:hydrogen symporter activity, transporter activity, auxin efflux transmembrane transporter activity; INVOLVED IN: response to cyclopentenone, root development, pattern specification process, auxin polar transport, longitudinal axis specification; LOCATED IN: plasma membrane, membrane; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Auxin efflux carrier, subgroup (InterPro:IPR014024), Auxin efflux carrier (InterPro:IPR004776); BEST Arabidopsis thaliana protein match is: Auxin efflux carrier family protein (TAIR:AT1G70940.1); Has 1748 Blast hits to 1583 proteins in 451 species: Archae - 37; Bacteria - 919; Metazoa - 14; Fungi - 2; Plants - 486; Viruses - 0; Other Eukaryotes - 290 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT1G23080.2

Model type
Protein coding
Short Description
Auxin efflux carrier family protein
Curator Summary
Encodes a novel component of auxin efflux that is located apically in the basal cell and is involved during embryogenesis in setting up the apical-basal axis in the embryo. It is also involved in pattern specification during root development. In roots, it is expressed at lateral and basal membranes of provascular cells in the meristem and elongation zone, whereas in the columella cells it coincides with the PIN3 domain. Plasma membrane-localized PIN proteins mediate a saturable efflux of auxin. PINs mediate auxin efflux from mammalian and yeast cells without needing additional plant-specific factors. The action of PINs in auxin efflux is distinct from PGPs, rate-limiting, specific to auxins and sensitive to auxin transport inhibitors. PINs are directly involved of in catalyzing cellular auxin efflux.
Computational Description
PIN-FORMED 7 (PIN7); FUNCTIONS IN: auxin:hydrogen symporter activity, transporter activity, auxin efflux transmembrane transporter activity; INVOLVED IN: response to cyclopentenone, root development, pattern specification process, auxin polar transport, longitudinal axis specification; LOCATED IN: plasma membrane, membrane; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Auxin efflux carrier, subgroup (InterPro:IPR014024), Auxin efflux carrier (InterPro:IPR004776); BEST Arabidopsis thaliana protein match is: Auxin efflux carrier family protein (TAIR:AT1G70940.1); Has 700 Blast hits to 605 proteins in 126 species: Archae - 0; Bacteria - 174; Metazoa - 8; Fungi - 2; Plants - 457; Viruses - 0; Other Eukaryotes - 59 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT1G23080.3

Model type
Protein coding
Short Description
Auxin efflux carrier family protein
Curator Summary
Encodes a novel component of auxin efflux that is located apically in the basal cell and is involved during embryogenesis in setting up the apical-basal axis in the embryo. It is also involved in pattern specification during root development. In roots, it is expressed at lateral and basal membranes of provascular cells in the meristem and elongation zone, whereas in the columella cells it coincides with the PIN3 domain. Plasma membrane-localized PIN proteins mediate a saturable efflux of auxin. PINs mediate auxin efflux from mammalian and yeast cells without needing additional plant-specific factors. The action of PINs in auxin efflux is distinct from PGPs, rate-limiting, specific to auxins and sensitive to auxin transport inhibitors. PINs are directly involved of in catalyzing cellular auxin efflux.
Computational Description
PIN-FORMED 7 (PIN7); FUNCTIONS IN: auxin:hydrogen symporter activity, transporter activity, auxin efflux transmembrane transporter activity; INVOLVED IN: response to cyclopentenone, root development, pattern specification process, auxin polar transport, longitudinal axis specification; LOCATED IN: plasma membrane, membrane; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Auxin efflux carrier, subgroup (InterPro:IPR014024), Auxin efflux carrier (InterPro:IPR004776); BEST Arabidopsis thaliana protein match is: Auxin efflux carrier family protein (TAIR:AT1G70940.1); Has 1749 Blast hits to 1582 proteins in 451 species: Archae - 37; Bacteria - 920; Metazoa - 12; Fungi - 2; Plants - 486; Viruses - 0; Other Eukaryotes - 292 (source: NCBI BLink).
Link
InterPro Scan - TAIR