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ET13782

CSHL Arabidopsis genetrap mutant lines

Original: ET13782

Phenotypes

Morphological phenotypes

Specific phenotypes were NOT observed.

Original: ET13782

Determination of insertion point(s)

  • 3 prime edge [+] show sequence

    1. Chromosome: 1; Position: 24167258 [Map]; Strand: w
      • AT1G65060.1 - [+] show detail - 4-coumarate:CoA ligase 3
        Distance: 0; Region: exon7 (3UTR)
      • AT1G65060.2 - [+] show detail - 4-coumarate:CoA ligase 3
        Distance: 0; Region: exon6 (3UTR)
    Flanking Genome Sequence: ET13782 - 3-prime edge 
    AACGGTAGAG GTATTTTACC GACCGTTACC GACCGTTTTC ATCCCTAGCT 
    TAAGATACTG AATTATTTTG GATCAGTTTA TTTCAATGAT TTTTGTTTTC 
    TTTATGAAAG TTAGAGAATG ATTAAATAAA TCATCTATAT AAACAGACAT 
    ATATGAAACG TAATAAATAA AGAGACATAT GAATTGTTTC TCTACGACTA 
    TAGCGATTAT CGATGGTGAT ATGATATTCT AGTTTTTTTC AAAATTTGTG 
    GAGAGATATA AAGTCTTGTT TCAGAACACT ATACCATCTC AATCTTTTTC 
    AGTAATTAAA TTGACATAAT GACAATTTGA ACTCTATGAG AAAAAGTGTA 
    ACTTAGCATA AACATTTTAT TTATAGATCC CCAATTTTGT CGCATGGCTT 
    CTCACTTGAG CTAGAGAGTT TTCTCGAGGC ATCATATCGA CACATTAAAC 
    TTACACCATC GAAATCTTGA AAGAAGGTTA TGTATATGAC CAATGTTTGT 
    CCGATAATGT GACCACTTTA ATAATATTGT ATTCTTATCT ATTTCAGCCA 
    TGGGGTTTTA TAGGATTTTA ATAAGTATTT GTGTGGTTTT AATGATTCTA 
    ATATTATTTT CCAAGTATAT TTGTAGTTTC TGAGTCTTTA TAGTTTTTTG 
    GGCACTGAAA ACCACAAATG GTGCAAAACA AGC

AT1G65060.1

Model type
Protein coding
Short Description
4-coumarate:CoA ligase 3
Curator Summary
encodes an isoform of 4-coumarate:CoA ligase (4CL), which is involved in the last step of the general phenylpropanoid pathway. mRNA levels are not induced in response to wounding or to fungal infection by P. parasitica. mRNA is expressed in flowers, to a lesser degree in mature leaves and siliques and marginally in seedling roots and bolting stems of mature plants. The catalytic efficiency was in the following (descending) order: p-coumaric acid, caffeic acid, ferulic acid, cinnamic acid and 5-OH-ferulic acid. At4CL3 was unable to use sinapic acid as substrate.
Computational Description
4-coumarate:CoA ligase 3 (4CL3); CONTAINS InterPro DOMAIN/s: AMP-binding, conserved site (InterPro:IPR020845), AMP-dependent synthetase/ligase (InterPro:IPR000873); BEST Arabidopsis thaliana protein match is: 4-coumarate:CoA ligase 2 (TAIR:AT3G21240.1); Has 85516 Blast hits to 77968 proteins in 3765 species: Archae - 1155; Bacteria - 54010; Metazoa - 3516; Fungi - 4668; Plants - 2834; Viruses - 1; Other Eukaryotes - 19332 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT1G65060.2

Model type
Protein coding
Short Description
4-coumarate:CoA ligase 3
Curator Summary
encodes an isoform of 4-coumarate:CoA ligase (4CL), which is involved in the last step of the general phenylpropanoid pathway. mRNA levels are not induced in response to wounding or to fungal infection by P. parasitica. mRNA is expressed in flowers, to a lesser degree in mature leaves and siliques and marginally in seedling roots and bolting stems of mature plants. The catalytic efficiency was in the following (descending) order: p-coumaric acid, caffeic acid, ferulic acid, cinnamic acid and 5-OH-ferulic acid. At4CL3 was unable to use sinapic acid as substrate.
Computational Description
4-coumarate:CoA ligase 3 (4CL3); CONTAINS InterPro DOMAIN/s: AMP-binding, conserved site (InterPro:IPR020845), AMP-dependent synthetase/ligase (InterPro:IPR000873); BEST Arabidopsis thaliana protein match is: 4-coumarate:CoA ligase 2 (TAIR:AT3G21240.1); Has 78268 Blast hits to 71395 proteins in 3651 species: Archae - 1111; Bacteria - 51774; Metazoa - 3299; Fungi - 4373; Plants - 2538; Viruses - 1; Other Eukaryotes - 15172 (source: NCBI BLink).
Link
InterPro Scan - TAIR