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ET13974

CSHL Arabidopsis genetrap mutant lines

Original: ET13974

Phenotypes

Morphological phenotypes

Original description

  • embryo lethal (unconfirmed)
Anatomical entity
/structure development stage		 Phenotypes
seed (PO:0009010)
  • present in fewer numbers in organism (PATO:0001997)

Original: ET13974

Determination of insertion point(s)

  • 3 prime edge [+] show sequence

    1. Chromosome: 2; Position: 5649262 [Map]; Strand: w
      • AT2G13550.1 - [+] show detail - unknown protein; Has 23 Blast hits to 20 proteins in 10 species: Archae - 0; ...
        Distance: 0; Region: exon1 (CDS)
      • AT2G13560.1 - [+] show detail - NAD-dependent malic enzyme 1
        Distance: -702; Region: promoter
    Flanking Genome Sequence: ET13974 - 3-prime edge 
    CGGTAGAGGT ATTTTACCGA CCGTTACCTA CCGTTTTCAT CCCTAAACAG 
    AACCACCTTC CAAAAATAAC CACCACCCAG ACGAGCCGTC GCCTAGTCCA 
    CAACAAGAAC GTCACATACC AGATCAAAAC CCATCACCAG AGGCATCCAC 
    CAACTCAAAA CGACTTCATC TTCCTCGACA AACACATAGA ACACGACGGG 
    GGAAGACAAA AGGAAACAAT TCCATATCGA GACAAACACT GACATAGCTT 
    CAACTGACAA AACCAGATCA AGATCTGGAA ATCAATCAAA ACCCCAGCCA 
    TATAGAGCCA AAATTGAAAC AGAGCCTCAA AACCGGAGCT GCCAACAACA 
    CAGAGACCAA CCTCCGAAAC AGATATAGAA GAGAAATTGG TCCCCGCACT 
    CCCCAACACC ATCAACCCTA CAACCATATC ACCATAAAAA CGAGAAGGAA 
    GAAATCTACA GACGAAATTA AACAAAGACG CACAAGGCCA GAATCAGTGC 
    TAAGGTAGCC ACCGCATTCT AGCCTCAAAA CTGCAAAACC TCTTTAGACG 
    GCGGCTGGTT TTGCGAGAGA GAAAACTAGG TTTTTTTTTG TTTTTTTGAG 
    TTACTTACTC GCTTACATGA GTAACTTTTT TTTTCTTCGA AAAAACTTAT 
    CTCCGCTTTA CGTCAAACTT GAATCTTTGC TAGGGGCAAG GGAAGGCATG 
    ATCAG

  • 5 prime edge [+] show sequence

    1. Chromosome: 2; Position: 5649283 [Map]; Strand: w
      • AT2G13550.1 - [+] show detail - unknown protein; Has 23 Blast hits to 20 proteins in 10 species: Archae - 0; ...
        Distance: 0; Region: exon1 (CDS)
      • AT2G13560.1 - [+] show detail - NAD-dependent malic enzyme 1
        Distance: -681; Region: promoter
    Flanking Genome Sequence: ET13974 - 5-prime edge 
    TCATCTACTT TCATCCTGGT TCTGTTACTG GTGGCTGTTT GGAGGCGGTT 
    TAGGTTGTCT TCCGGTGCAG GTATTTGGCT AAAACTGGGT TGGCGAATGC 
    CCGCGTGCAA GAGGTGTTTG TCTTATTTGG CTTGTCATTT GGCGATTCTC 
    AAGGGCTTCC CTTGTTCCTA GTTTATTTTC ATCTTCTCCG GCGACTTGGT 
    ATGGTGTGAT TGTTGTCTCT CTAGTATCAT TTGGTTCTTG CTTTCGTTCC 
    TGGCGGTTTG ACTTCAGAAA GATGTCTTAA TTTTGTGGCA TTACCAGTCT 
    TATTGCTGCT TCACGTGTTC TTTTGGAAAG CTTAGTGAGA ACCTCTATGG 
    AGCAGTTGGT GAGCTTAGTG CTTTGGTCTC AACTCTCAAT CTCTTCCTTT 
    TGCGAGTTTA TCTTGTTGTT TTTACTTTTC TTGGTAATTT AGAAGTCTGG 
    TTTTTAGGGA TTAGTTTTCC TCCTTTTGGT CTTTGTCTCT AGGGATTTTT 
    GTTTATCCGC CGGCTTATGT ATGATTTTCG ACTTGTATGT TCTCGTTGCA 
    ATTCTAATAA GATAAAAAAA AA

AT2G13550.1

Model type
Protein coding
Computational Description
unknown protein; Has 23 Blast hits to 20 proteins in 10 species: Archae - 0; Bacteria - 0; Metazoa - 10; Fungi - 0; Plants - 4; Viruses - 0; Other Eukaryotes - 9 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT2G13560.1

Model type
Protein coding
Short Description
NAD-dependent malic enzyme 1
Curator Summary
Encodes an NAD-dependent malic enzyme (NAD-ME) that does not act on oxaloacetate, indicating that it belongs to EC 1.1.1.39. It is a member of the alpha family of NAD-MEs in plants. It appears to function as a homodimer or as a heterodimer with the beta-type NAD-ME2 (At4g00570). NAD-ME1 transcript and protein levels are higher during the night than during the day.
Computational Description
NAD-dependent malic enzyme 1 (NAD-ME1); FUNCTIONS IN: in 7 functions; INVOLVED IN: response to salt stress, malate metabolic process; LOCATED IN: mitochondrion, chloroplast; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Malic enzyme, NAD-binding (InterPro:IPR012302), Malic oxidoreductase (InterPro:IPR001891), Malic enzyme, conserved site (InterPro:IPR015884), Malic enzyme, N-terminal (InterPro:IPR012301), NAD(P)-binding domain (InterPro:IPR016040); BEST Arabidopsis thaliana protein match is: NAD-dependent malic enzyme 2 (TAIR:AT4G00570.1); Has 9465 Blast hits to 9448 proteins in 2440 species: Archae - 143; Bacteria - 6328; Metazoa - 607; Fungi - 221; Plants - 457; Viruses - 0; Other Eukaryotes - 1709 (source: NCBI BLink).
Link
InterPro Scan - TAIR