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ET14126

CSHL Arabidopsis genetrap mutant lines

Original: ET14126

Phenotypes

Morphological phenotypes

Specific phenotypes were NOT observed.

Original: ET14126

Determination of insertion point(s)

  • 3 prime edge [+] show sequence

    1. Chromosome: 4; Position: 246703 [Map]; Strand: c
      • AT4G00570.1 - [+] show detail - NAD-dependent malic enzyme 2
        Distance: 0; Region: exon1 (5UTR)
    Flanking Genome Sequence: ET14126 - 3-prime edge
    ATGAAACGGT AGAGGTATTT TACCGACCGT TACCGACCGT TTTCATCCCT 
    AGTGTTCTCC TCCTCTTCTC CAGTAGAAGA AGCTGTGTCT GAGGAAGAGG
    GAAGATGTCT TTAAGAACAG CTGCCCGAAG AATAGTAAAA GGGTCAGACA
    AATAAATAAA GCTTTCCAAT TTTCTAAAAT TCTAAAGAAG AAATAATTCT
    TTTATTAAAA AGTCAATAAA TACAAGAAAA CAAAAAGAAG TTGCCCGAAG
    GAAAACGGGC AAAGAGGGTA TAAAAGTAAA TGTGAATATA TAGATCGCCA
    CGTGGACGGT TGTGAATCAT TTTCCAGGCA TATTGGTGAT GAGACTAGAG
    GTCAAAGGAT ATTGGCTAAC TCACCGTTAG ATCAATGACA CGTGTATCCG
    CGTTGTGGTG TTTCCGCTTT TACCCATGCG TGTGCCTCTG GTAACAAATA
    TTACGTCTTC CTAAAATAAA AAAAGTACCA AGTAGAGAAA TTAGAGAAAA
    GGAAAGGTAA TATATAAATT TGAGATTCGA GTGTTTTAAG TGTAAACCAT
    TCTTTTACAA AAAAATATTC GCTGCGGCGA CTTGACACCG TCAGCTTTTA
    ACTCTTAAA

AT4G00570.1

Model type
Protein coding
Short Description
NAD-dependent malic enzyme 2
Curator Summary
Encodes an NAD-dependent malic enzyme (NAD-ME) that does not act on oxaloacetate, indicating that it belongs to EC 1.1.1.39. It is a member of the beta family of NAD-MEs in plants. It appears to function as a homodimer or as a heterodimer with the alpha-type NAD-ME2 (At2g13560). NAD-ME2 transcript and protein levels are higher during the night than during the day.
Computational Description
NAD-dependent malic enzyme 2 (NAD-ME2); FUNCTIONS IN: in 7 functions; INVOLVED IN: malate metabolic process; LOCATED IN: mitochondrion, chloroplast; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Malic enzyme, NAD-binding (InterPro:IPR012302), Malic oxidoreductase (InterPro:IPR001891), Malic enzyme, conserved site (InterPro:IPR015884), Malic enzyme, N-terminal (InterPro:IPR012301), NAD(P)-binding domain (InterPro:IPR016040); BEST Arabidopsis thaliana protein match is: NAD-dependent malic enzyme 1 (TAIR:AT2G13560.1); Has 9343 Blast hits to 9326 proteins in 2439 species: Archae - 143; Bacteria - 6323; Metazoa - 607; Fungi - 223; Plants - 463; Viruses - 0; Other Eukaryotes - 1584 (source: NCBI BLink).
Link
InterPro Scan - TAIR